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991.
992.
During a longitudinal study of vector biology and malaria transmission in western Venezuela, adult mosquitoes were collected by different methods and their efficiency was compared with human landing catches. CDC light traps, a double-net, a calf-baited trap and collection of resting mosquitoes on vegetation were tested. These methods did not prove to be effective substitutes for human landing catches. 相似文献
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996.
Osteoclastic features of multinucleated giant cells responding to synthetic hydroxyapatite implanted in rat jaw bone. 总被引:2,自引:0,他引:2
N Takeshita T Akagi M Yamasaki T Ozeki T Nojima Y Hiramatsu N Nagai 《Journal of electron microscopy》1992,41(3):141-146
Multinucleated giant cells (MGCs) that responded to synthetic hydroxyapatite (HAP) implanted in rat mandibles were studied with electron microscopy. HAP used in this study sintered at 200 degrees C (HAP200) and at 125 degrees C (HAP1250) after the synthesis by a wet method. One to three weeks after the intraosseous implantation of HAP, MGCs responding to HAP200 had not only well-developed ruffled border and the clear zone but well-developed perinuclear Golgi complex, many mitochondria and vesicles in their cytoplasms. MGCs responding to HAP1250 had the clear zone, but not the ruffled border although they showed similar cytoplasmic features to those of MGCs responding to HAP200. They merely extended short slender cytoplasmic processes to HAP1250. These results suggest that although osteoclast-like MGCs respond to HAP implanted in the bone, the development of the ruffled border-clear zone system depends on physicochemical properties of HAP. 相似文献
997.
Molecular cloning of a canine metallothionein cDNA 总被引:1,自引:0,他引:1
A canine metallothionein cDNA obtained from the liver of a cadmium-treated beagle was cloned and sequenced. Asn at position 4 conserved among all mammalian metallothionein-1 and metallothionein-2 is replaced by Asp in the canine metallothionein cDNA clone. Because the acidic amino acid doesn't exist at either position 10 or 11 in the deduced amino acid sequence, it is supposed that this cDNA is derived from canine metallothionein-1 mRNA. Northern blot analysis using the cDNA as a probe revealed the induction of the canine metallothionein mRNA expression in the liver and kidney of a cadmium-treated beagle. Thus, the canine metallothionein cDNA obtained in the present study should provide an useful tool for the molecular investigation of metallothionein in dog. 相似文献
998.
JS Lee S Scala Y Matsumoto B Dickstein R Robey Z Zhan G Altenberg SE Bates 《Canadian Metallurgical Quarterly》1997,65(4):513-526
MCF-7 human breast cancer cells selected in Adriamycin in the presence of verapamil developed a multidrug resistant phenotype, which was characterized by as much as 100,000-fold resistance to mitoxantrone, 667-fold resistance to daunorubicin, and 600-fold resistance to doxorubicin. Immunoblot and PCR analyses demonstrated no increase in MDR-1 or MRP expression in resistant cells, relative to parental cells. This phenotype is similar to one previously described in mitoxantrone-selected cells. The cells, designated MCF-7 AdVp, displayed a slower growth rate without alteration in topoisomerase II alpha level or activity. Increased efflux and reduced accumulation of daunomycin and rhodamine were observed when compared to parental cells. Depletion of ATP resulted in complete abrogation of efflux of both daunomycin and rhodamine. No apparent alterations in subcellular daunorubicin distribution were observed by confocal microscopy. No differences were noted in intracellular pH. Molecular cloning studies using DNA differential display identified increased expression of the alpha subunit of the amiloride-sensitive sodium channel in resistant cells. Quantitative PCR studies demonstrated an eightfold overexpression of the alpha subunit of the Na+ channel in the resistant subline. This channel may be linked to the mechanism of drug resistance in the AdVp cells. The results presented here support the hypothesis that a novel energy-dependent protein is responsible for the efflux in the AdVp cells. Further identification awaits molecular cloning studies. 相似文献
999.
H Matsubara N Oya Y Suzuki S Kajiura K Suzumori Y Matsuo T Suzuki T Hashimoto 《Canadian Metallurgical Quarterly》1997,12(5):306-308
Both choledochal cyst and congenital biliary atresia (type I cyst) may share the same ultrasonographic pattern. We report 2 cases which were shown to have cystic structures on the upper abdomen by antenatal ultrasonography. The size of the choledochal cyst found at 24 weeks' gestation increased steadily as gestational age advanced. But the size of the cyst in congenital biliary atresia found at 29 weeks' gestation remained unchanged throughout the remaining pregnancy. Cyst enlargement, therefore, may suggest the possibility of a choledochal cyst. 相似文献
1000.
This paper presents stability results for discrete-time model-based predictive control system subject to an input amplitude constraint. It is shown that the input amplitude constrained control system may provide a stable control system in the sense of BIBO when the system to be controlled is of a class of the system poles which consist of multiple integrators and a stable polynomial. The solution of Diophantine equations and their properties are addressed. Simulation study is also carried out and it is shown that the output of the system may converge to the reference signal for certain degree of constraints. 相似文献