Purification and renaturation of recombinant human lymphotoxin (tumour necrosis factor beta) expressed in Escherichia coli as inclusion bodies

High level expression of recombinant human tumour necrosis factor β (rh TNF-β) in Escherichia coli results in the formation of two portions of protein, namely soluble active protein and insoluble protein which is inactive and aggregates in the form of inclusion bodies (IBs). In this study, a procedure for purification and renaturation of rh TNF-β from inclusion bodies has been designed and verified experimentally with a product purity of more than 90% and a recovery of about 30%. The procedure includes washing of IBs with specific wash buffer (Triton X-100/EDTA/lysozyme/PMSF), their solubilization with 8 mol dm^?3 alkaline urea, purification with ion-exchange columns, refolding with renaturation buffer and finally concentration and desalination with an ultrafiltration membrane. The characteristics of the renatured protein were identical with those of purified protein from the soluble fraction as demonstrated by (1) SDS-PAGE, (2) cytotoxic activity on mouse L929 cells, (3) N-terminal amino acid sequence, and (4) gel filtration chromatography.     

微机模拟处理pH络合滴定法研究——Ⅱ.终点确定方法和最佳实验条件模拟估计程序

本文利用简便的滴定过程模型,确立了微机模拟处理实验数据,方便、准确地求解络合滴定等当点的方法。设计了最佳实验条件模拟估计程序。该法测定Zn~(2+),Pb~(2+),Cu~(2+),Fe~(3+)结果的相对误差Δ<1％。pH=6-7时,测定Ca~(2+)的相对误差为-1.61％;σ<0.001mmol,(N=5)。     

坑道用水下堵漏剂的研制

坑道水下堵漏剂的研制包括F－1、F－2和F－3型。其中F－1是一种有机堵漏剂，它可以在水中固化；F－2是一种无机防水堵漏剂，施工方便；F－3是速凝型，它可在瞬间固化，能对坑道的涌水口进行堵漏。     

Survey of nitrate and nitrite contents of vegetables grown in Korea.

A scientific basis for the evaluation of the risk to public health arising from excessive dietary intake of nitrate in Korea is provided. The nitrate () and nitrite () contents of various vegetables (Chinese cabbage, radish, lettuce, spinach, soybean sprouts, onion, pumpkin, green onion, cucumber, potato, carrot, garlic, green pepper, cabbage and Allium tuberosum Roth known as Crown daisy) are reported. Six hundred samples of 15 vegetables cultivated during different seasons were analysed for nitrate and nitrite by ion chromatography and ultraviolet spectrophotometry, respectively. No significant variance in nitrate levels was found for most vegetables cultivated during the summer and winter harvests. The mean nitrates level was higher in A. tuberosum Roth (5150 mg kg(-1)) and spinach (4259 mg kg(-1)), intermediate in radish (1878 mg kg(-1)) and Chinese cabbage (1740 mg kg(-1)), and lower in onion (23 mg kg(-1)), soybean sprouts (56 mg kg(-1)) and green pepper (76 mg kg(-1)) compared with those in other vegetables. The average nitrite contents in various vegetables were about 0.6 mg kg(-1), and the values were not significantly different among most vegetables. It was observed that nitrate contents in vegetables varied depending on the type of vegetables and were similar to those in vegetables grown in other countries. From the results of our studies and other information from foreign sources, it can be concluded that it is not necessary to establish limits of nitrates contents of vegetables cultivated in Korea due to the co-presence of beneficial elements such as ascorbic acid and alpha-tocopherol which are known to inhibit the formation of nitrosamine.     

带肋钢筋套筒挤压连接的质量控制

针对太原可口可乐综合厂房主筋采用φ28、φ32三级钢存在的可焊性差的问题，提出了在施工中采用套筒挤压连接的方式，并对该方法的操作要点及质量控制措施进行了总结。     

Plasma properties of laser-ablated St target in air

Ｐｌａｓｍａｐｒｏｐｅｒｔｉｅｓｏｆｌａｓｅｒ－ａｂｌａｔｅｄＳｔｔａｒｇｅｔｉｎａｉｒＷａｎｇＸｉａｎｇ－Ｔａｉ（王象泰）；ＭａｎＢａｏ－Ｙｕａｎ（满宝元）；ＷａｎｇＧｏｎｇ－Ｔａｎｇ（王公堂）；ＦａｎＸｉ－Ｊｕｎ（樊锡君）；ＷａｎｇＪｕｎ（王军）...     

北京谱仪Ⅱ（BESⅡ）顶点探测器数据获取系统

北京谱仪（ＢＥＳⅡ）顶点探测器数据获取系统是北京谱仪数据获取系统的一部分，该子系统电子学采用快总线标准，本文描述了该系统的硬件结构和软件系统，软件包括快总线系统的微码软件和上层控制软件，该系统的死时间为１．５ｎｓ，对ＢＥＳⅡ系统总死时间的贡献小于０．５ｍｓ。     

A micromachined electrochemical sensor for free chlorine monitoring in drinking water.

In this work, we designed, fabricated and tested a disposable, flow-through amperometric sensor for free chlorine determination in water. The sensor is based on the principle of an electrochemical cell. The substrate, as well as the top microfluidic layer, is made up of a polymer material. The advantages include; (a) disposability from low cost; (b) stable operation range from three-electrode design; (c) fluidic interconnections that provide on line testing capabilities; and (d) transparent substrate which provides for future integration of on-chip optics. The sensor showed a good response and linearity in the chlorine concentration ranging from 0.3 to 1.6 ppm, which applies to common chlorination process for drinking water purification.     

一幅画

从吃饱到吃好、吃精至吃的环境,人们对生活的要求逐渐趋向艺术化,餐馆老板会把环境设计与经营放在同等地位,所以好的餐饮空间层出不穷,也是社会发展的必然产物。位于宁波老外滩的瀚珍舫餐馆,建筑一层的面积较小,二、三层呈狭长形,做为餐馆这样的建筑条件并不理想,设计师把包厢和雅座设置在两边,这样走廊显得更幽深,柱子阵列得更有气势。拆除原先的楼梯,做了一部上下错位且不规则楼梯,曲折向上,增强了空间的层次。通顶的储酒柜,从三楼悬下的八米长吊灯,把一至三楼不落痕迹地融为一体。要设计一个好作品,设计师的构想需要不停地游走。碰撞的激情仿佛是一幅画,渐渐地,它的轮廓清晰了,色彩明朗了,它的主题神秘而遥不可及,每个人的心扉都有不同的暗香飘逸。掩映在现代手法中的精致、深沉从骨子里透出古典优雅     

Self-aligned diffusion technique for n-InP JFETs

A self-aligned diffusion process has been demonstrated in the fabrication of n-InP JFETs on semi-insulating InP. This process utilises the anisotropic and selective etching properties of InP/GaInAs materials. Using this technique, sub-micrometre gate lengths can be achieved even with conventional photolithography. Devices with trans-conductance of >35mS/mm, leakage current of < 10nA/mm and gate capacitance of <0.7pF/mm have been fabricated with a cutoff frequency of 7 GHz.