Characterization of a Thermostable and Surfactant-Tolerant Chondroitinase B from a Marine Bacterium Microbulbifer sp. ALW1

Chondroitinase plays an important role in structural and functional studies of chondroitin sulfate (CS). In this study, a new member of chondroitinase B of PL6 family, namely ChSase B6, was cloned from marine bacterium Microbulbifer sp. ALW1 and subjected to enzymatic and structural characterization. The recombinant ChSase B6 showed optimum activity at 40 °C and pH 8.0, with enzyme kinetic parameters of K_m and V_max against chondroitin sulfate B (CSB) to be 7.85 µg/mL and 1.21 U/mg, respectively. ChSase B6 demonstrated thermostability under 60 °C for 2 h with about 50% residual activity and good pH stability under 4.0–10.0 for 1 h with above 60% residual activity. In addition, ChSase B6 displayed excellent stability against the surfactants including Tween-20, Tween-80, Trion X-100, and CTAB. The degradation products of ChSase B6-treated CSB exhibited improved antioxidant ability as a hydroxyl radical scavenger. Structural analysis and site-directed mutagenesis suggested that the conserved residues Lys248 and Arg269 were important for the activity of ChSase B6. Characterization, structure, and molecular dynamics simulation of ChSase B6 provided a guide for further tailoring for its industrial application for chondroitin sulfate bioresource development.     

环江油田巴19区块长7段钙夹层评价与大斜度井分段压裂优化

为实现最大化油藏接触,提高油藏产出水平,长庆环江油田巴19区块长7油藏采用大斜度井开发方式。在初期压裂改造中,由于忽视了层内钙质夹层对压裂缝高的影响,导致实际裂缝的几何形态与设计产生巨大偏差,出现了邻井裂缝串通及生产高含水等问题。本文通过研究油藏剖面测井特征,在细分层位基础上分析纵向应力分布,研究“复合层效应”影响裂缝缝高延伸的关键因素,优化了分段压裂方案,获得了更好的水力裂缝几何形态和尺寸。技术调整后现场压裂工艺质量获得提高,长7段油藏压裂改造质量优良率100%,大大提高了该区块的开发水平。     

具有输入饱和的近空间飞行器鲁棒控制

针对近空间飞行器这一类存在外部扰动,输入饱和和参数不确定的多输入多输出线性系统,提出了一种基于干扰观测器的抗饱和鲁棒控制方案.将干扰观测器与抗饱和控制技术相结合,从而消除系统存在的未知外部扰动、输入饱和和不确定性对系统控制的影响.首先,设计干扰观测器对线性外部系统产生的未知扰动进行估计.然后根据干扰观测器输出,通过超前抗饱和方法设计抗饱和补偿器,并将其加入到鲁棒控制器的设计中,保证闭环系统存在输入饱和、未知外部扰动和参数不确定情况下的稳定性.为便于设计,干扰观测器、抗饱和补偿器和控制器设计矩阵均通过求解线性矩阵不等式得到.最后,将提出的鲁棒抗饱和控制方法应用于近空间飞行器,仿真结果验证了该控制方案的有效性.     

纤维增强轻质混凝土在楼地面保温的应用

介绍了纤维增强轻质混凝土特点及其在楼地面保温隔热上的施工工艺和施工方法,同时阐述了有关注意事项。     

探索农村公路管理养护体制改革新思路

按照国务院办公厅《关于印发农村公路管理养护体制改革方案的通知》(国办发〔2005〕49号)与安徽省政府办公厅《关于推进农村公路管理养护体制改革的意见》(皖政办〔2007〕76号)文件精神,农村公路管理养护体制改革是摆在各级交通主管部门深化改革面前的一项重要工作。文章结合蚌埠实际,加以探索农村公路管理养护新体制、新机制。     

Cell evolution and compressive properties of styrene–butadiene–styrene toughened and calcium carbonate reinforced polystyrene extrusion foams with supercritical carbon dioxide

Polystyrene (PS) foams have been used in various fields, whereas its broader application is limited by its low mechanical strength and brittle features. In this study, styrene–butadiene–styrene (SBS) and calcium carbonate (CaCO₃) nanoparticles were melt‐blended with PS and extrusion‐foamed with supercritical carbon dioxide as a blowing agent to simultaneously toughen and reinforce PS foams. Under the same foaming conditions, the addition of SBS and CaCO₃ was shown to have a significant influence on the cell structure and the compressive properties of the composite foams. We found that the cell structure evolution was highly correlated with the system viscosity. When the rubbery‐phase SBS content was 20%, the cell diameter decreased by 20.7%, and the compressive modulus was enhanced by 289.5%. With the further addition of 5% rigid CaCO₃ nanoparticles, the cell diameter was further reduced by 72.2% and the compressive modulus was improved by 379.2%. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133, 43508.     

Circulating Organ-Specific MicroRNAs Serve as Biomarkers in Organ-Specific Diseases: Implications for Organ Allo- and Xeno-Transplantation

Different cell types possess different miRNA expression profiles, and cell/tissue/organ-specific miRNAs (or profiles) indicate different diseases. Circulating miRNA is either actively secreted by living cells or passively released during cell death. Circulating cell/tissue/organ-specific miRNA may serve as a non-invasive biomarker for allo- or xeno-transplantation to monitor organ survival and immune rejection. In this review, we summarize the proof of concept that circulating organ-specific miRNAs serve as non-invasive biomarkers for a wide spectrum of clinical organ-specific manifestations such as liver-related disease, heart-related disease, kidney-related disease, and lung-related disease. Furthermore, we summarize how circulating organ-specific miRNAs may have advantages over conventional methods for monitoring immune rejection in organ transplantation. Finally, we discuss the implications and challenges of applying miRNA to monitor organ survival and immune rejection in allo- or xeno-transplantation.     

激光成形修复Inconel 625合金的工艺特性和组织研究

对激光成形修复Inconel 625合金的工艺特性以及不同区域的组织进行了研究。结果表明:激光成形修复Inconed625合金的工艺范围较宽;激光功率主要影响单道修复层的宽度;扫描速度对单道修复层的尺寸影响较为显著,而送粉率的影响较小。基材的相组成包括γ(Ni-Cr)基体相、大尺寸(约10μm)块状MC型碳化物(M为Nb和Ti)、沿晶界析出的小尺寸(约0.5μm)块状MC型碳化物以及不规则形状的Laves相。修复区组织为呈外延生长的柱状枝晶,相组成为γ基体相、沿枝晶界析出的Laves相以及少量MC型碳化物。层与层界面处Laves相析出急剧增加形成层间过渡区。     

Design of a europium-activated lithium-silicate cyan phosphor via cation substitution

Lithium-containing silicates have been considered as a considerable alternative for luminescent materials. In this study, a novel cyan-emitting phosphor, Na₃LiHf₂Si₃O₁₂: Eu²⁺, was successfully synthesized via cationic substitution with Na₄Hf₂Si₃O₁₂: Eu²⁺ as the initial model. The crystal structure, morphology, and luminescence performance of Na_4-xLi_xHf₂Si₃O₁₂: Eu²⁺ were investigated in detail. The substitution of Li⁺ for Na⁺ site causes a significant blue-shift of the emission band in the range of 550–500 nm and a smaller full width at half maximum. As a result, a cyan phosphor Na₃LiHf₂Si₃O₁₂: Eu²⁺ that can be effectively excited by near-ultraviolet and high-energy beams is obtained. The mechanism of emission regulation was proposed based on the transformation of crystal structure and luminescence performance. In addition, the thermal quenching and cathodoluminescence behaviors were also studied. The results show that cation substitution is an effective method to design new lithium-containing silicate phosphors.