A novel green synthesis of zero valent iron nanoparticles (NZVI) using three plant extracts and their efficient application for removal of Cr(VI) from aqueous solutions

In the present study, NZVI particles were synthesized from the plant extracts including Rosa damascene (RD), Thymus vulgaris (TV), and Urtica dioica (UD). The FTIR arspectshowed that polyphenols, proteins and organic acids which serve as reducing and stabilizing agents play a significant role in the synthesis of NPs and reduce the possibility of aggregation of NPs compared to chemical techniques of NPs synthesis. The amount and type of compounds in plant extracts affect the structure and also agglomeration of NPs after adsorption process. Based on the results, the highest removal efficiency occurred at pH 2. With increase in contact time and amount of dose, the percentage removal increases. Inversely, increase of initial concentration of Cr(VI) decreases the removal efficiency of the contaminant. These nanoparticles have a high adsorption capacity. Accordingly, by applying a dose of 0.2 g/l and contact time of 10 min, the three NPs yielded >90% removal efficiency. Also, for 1 min contact time, the percentage removal was 94.87%, 83.48% and 86.8% for RD-Fe, UD-Fe and TV-Fe, respectively. By an increase to 25 min, the removal percentage reached to 100% for TV-Fe and UD-Fe. Moreover, 30 min was required to remove Cr(VI) completely by RD-F.     

Inflammation measurement and immunocharacterization of cell proliferation in an experimental model of proliferative vitreoretinopathy

An experimental model of proliferative vitreoretinopathy was developed in the rabbit eye by injecting a solution of human platelet-rich plasma. In this model we evaluated the progression with time of intraocular inflammation and the rate and origin of cell proliferation. A sterile solution adjusted to 107 platelets was injected into the right eye of a total of 46 pigmented and 14 albino rabbits. Animals were sequentially sacrificed at days 7, 14, 21 and 1 month after injection. Clinical evaluation of vitreoretinal proliferation, using a classification in six grades, and of anterior segment inflammation assessed by a Laser Flare Meter, were done for 1 month after injection, before histopathological analysis. Eighty percent of eyes developed tractional retinal detachment in 1 month. Histopathology showed intense cell migration and proliferation in the area of the ciliary body, as early as the seventh day, then further increasing rapidly. Infiltrates were composed of cytokeratin- and vimentin-expressing cells. Abnormal expression of vimentin was also found in ciliary and retinal epithelia and in M?ller cells. Inflammation measured by the Laser Flare Meter was maximal at day 11 and then reached a plateau at significantly higher levels than controls. Albino rabbits showed significantly lower grades of proliferation, as compared to pigmented rabbits. This study thus clarified some characteristics of experimental vitreoretinal proliferations that that proved similar to those in human diseases, such as the involvement of ciliary body and retinal pigment epithelium, the existence of inflammatory reactions preceding cell proliferation and strong changes in intermediate filaments. This may provide a simple and valuable model for antiproliferative assays and shed some light on the pathogenesis of intraocular proliferative disorders.     

Filter paper blood spot assay of human insulin-like growth factor I (IGF-I) and IGF-binding protein-3 and preliminary application in the evaluation of growth hormone status

To facilitate broader applications of insulin-like growth factor I (IGF-I) and IGF-binding protein-3 (IGFBP-3) analysis, we developed procedures for their measurements in extracts of whole blood dried on filter paper. A single 8-mm diameter filter paper disc containing about 13 microL blood was used. IGFBP-3 was efficiently extracted in a buffer within 1 h of incubation. IGF-I extraction involved incubation in buffer followed by acidification and neutralization steps. Blood spot assays showed intra- and interassay coefficients of variation (including interspot variations) of 5.4-16.7% for IGF-I and 6.6-11.7% for IGFBP-3; recoveries were 97 +/- 7.1% and 101 +/- 8.7%, respectively. Recoveries of IGF-I and IGFBP-3 in response to 4- to 8-fold variations in extraction buffer volume were 97 +/- 8.2% and 107 +/- 6.1%, respectively. Dried blood spot IGF-I and IGFBP-3 showed greater than 1-month stability at -20 C, 4 C, and room temperature and retained more than 65% of the immunoreactivity after approximately 1 month at 37 C. Both IGF-I and IGFBP-3 were contained within the plasma fraction of whole blood, and variations (mean +/- SD) in IGF-I (204 +/- 29 micrograms/L) and IGFBP-3 (4.4 +/- 0.48 mg/L) measured in extracts of dried blood spot with adjusted hematocrit of 0.2-0.62 were acceptable. IGF-I and IGFBP-3 in paired plasma and dried blood spot extracts of random samples (n = 46) showed excellent correlation (r > 0.94) with slopes of near unity. Compared to conventional methods, the filter paper procedures were equally effective in distinguishing IGF-I and IGFBP-3 levels in untreated GH receptor-deficient (n = 11) and age-matched normal controls (n = 16). We conclude that blood collected on filter paper is ideal for IGF-I and IGFBP-3 analysis and may find applications in pediatric and large scale infant screening programs.     

Identifying time of a monotonic change in the fraction nonconforming of a high-quality process

When a signal is detected by control charts, a search begins to identify and eliminate the sources of this signal. Knowing when a process has changed is very helpful for this purpose. The unknown special point that the process changed for the first time is referred to as change point. In this paper, we propose a maximum-likelihood estimator for the behavior model of the process fraction nonconforming in a high-quality process monitored with a cumulative count of conforming (CCC) control chart. We estimate the time of change without requiring the prior knowledge of the change type rather than we assume the type of change present belongs to a family of monotonic changes. Then, we compare the performance of the proposed change point estimator relative to estimators for the process fraction nonconforming change point derived under a single step and a linear trend change assumption. We do this for a number of monotonic change types following a signal from a CCC control chart. Finally, the application of the proposed change point estimator is shown through a real case.     

System reliability analysis of soil nail wall using random finite element method

Soil nail wall is a compound system which for safety margin determination, consideration of safety factors of its components and their correlations is required. In this paper, considering a real site using the random finite element method (RFEM), the reliability indices of global stability, lateral displacement stability, tensile strength, and pullout resistance stability as components of the soil nail wall system are obtained. In another section of the paper, using the sequential compounding method (SCM), the importance of the mentioned stability modes and their effects on system reliability and system probability of failure are represented. Results show that the most considerable interdependence is between the global and lateral displacement stabilities. Among the reliability indices of the components, the minimum one is attributed to the pullout resistance. Furthermore, the uppermost row of the nails has the most critical reliability index compared with the others. The locations of the slip surfaces and nail intersections varied from 0.05–0.90 of the nail length, which means that the uncertainty of the soil parameters has the most significant effect on the pullout resistance safety factor of the nails. The performance level of the soil nail wall decreases from below average to poor when the soil nail wall is considered to be a system with series components.

     

An investigation on the behavior of stiffened coupled shear walls considering axial force effect

In most of tall buildings, the main contribution of lateral loads is carried by coupled shear walls. In some cases, the necessary stiffness to withstand the lateral load may not be afforded due to low depth of connecting beams. In order to increase the capacity of the coupled shear walls, beams with high stiffness are added to the system at particular levels. Hence, stiffened coupled shear walls (SCSW) will be produced. Such walls are under axial load resulting from their weight, and this axial load affects the behavior of walls because of their excessive height. In this paper, a new method considering the effect of axial force for geometrically nonlinear analysis of the SCSW has been presented. A computer program has been developed in matlab , and numerical examples have been solved to demonstrate the reliability of this method. The results of the examples show the agreement between the present method and the other methods given in the literature. The effects of the various positions and rigidities of the stiffening beam on the internal forces and the lateral deflection of the structure considering axial force effect have also been investigated. Copyright © 2012 John Wiley & Sons, Ltd.     

Photodegradation of ciprofloxacin in water using photocatalyst of zinc oxide nanowires doped with copper and cerium oxides

Antibiotic pollution via wastewaters has led to many environmental problems. In this work, to remove ciprofloxacin which is an antibiotic from water, foil photocatalyst of zinc oxide nanowires doped with copper and cerium oxides was applied under UV light irradiation. An empirical model was developed to determine the photocatalyst activity using response surface methodology. The independent variables were the concentrations of precursors, copper and cerium nitrates in the coating solution. The F‐value and p‐value of the model showed the accuracy of the model. The statistical analysis indicated that copper oxide had larger effect on the response than cerium oxide. The optimized catalyst was determined and characterized using ICP, XRD, SEM and MIP techniques. The results of this work which are comparable to those of the previous studies have the advantage of easy separation of the photocatalyst from water without using a centrifuge.