Specific control of cell–material interactions: Targeting cell receptors using ligand-functionalized polymer substrates

Cells respond to their environment in complex and sometimes poorly understood ways. Protein, peptide and synthetic peptidomimetic ligands may all be used to stimulate cells via receptor signaling, using interactions that are often highly specific. Polymer substrates that present these ligands provide a promising way to control cell development, both for applications in biotechnology and for fundamental studies of cell biology. Here we review a large range of techniques that have been employed to create and characterize ligand-functionalized substrates, with a particular focus on techniques that allow specific and consistent stimulation.     

Inflammatory response on injection of chitosan/GP to the brain

Chitosan is a well-known biomaterial that, with the addition of glycerophosphate salt (GP), gels at physiological temperatures and therefore is useful for tissue engineering purposes. This study examines the procedure of injecting chitosan/ GP to the brain in order to form a gel track. The gel system and surgical technique were successful in this endeavour; however, on examining the inflammatory response to the material it was found that the chitosan/GP was wholly engulfed by macrophages within 7 days. This was determined by staining for both the gel and the macrophages, an important technique for localising injected material. The chitosan/GP-containing macrophages formed a neat tract at the lesion site, but after 45 days no chitosan/GP was found. It was concluded that, although chitosan/GP is present after implantation, it is not available for direct scaffolding in the brain.     

Range Compression and Waveform Optimization for MIMO Radar: A CramÉr–Rao Bound Based Study

A multi-input multi-output (MIMO) radar system, unlike standard phased-array radar, can transmit via its antennas multiple probing signals that may be correlated or uncorrelated with each other. This waveform diversity offered by MIMO radar enables superior capabilities compared with a standard phased-array radar. One of the common practices in radar has been range compression. We first address the question of ldquoto compress or not to compressrdquo by considering both the Cramer-Rao bound (CRB) and the sufficient statistic for parameter estimation. Next, we consider MIMO radar waveform optimization for parameter estimation for the general case of multiple targets in the presence of spatially colored interference and noise. We optimize the probing signal vector of a MIMO radar system by considering several design criteria, including minimizing the trace, determinant, and the largest eigenvalue of the CRB matrix. We also consider waveform optimization by minimizing the CRB of one of the target angles only or one of the target amplitudes only. Numerical examples are provided to demonstrate the effectiveness of the approaches we consider herein.     

Cure kinetics and thermomechanical properties of thermally stable photopolymerized dimethacrylates

A range of dimethacrylates with varying backbone flexibility were partially photocured to various conversions using p‐xylylene bis‐(N,N‐diethyldithiocarbamate) as a photoiniferter and their glass transition region investigated by dynamic mechanical thermal analysis. For isothermally cured samples, the final degree of conversion was found to increase as the length of the spacer group in the monomer was increased or as the crosslink density in the resin was lowered due to the reduced glass transition temperature which allowed greater mobility and, hence, higher cure. Increasing the curing temperature also resulted in a higher degree of conversion as the network was able to polymerize further before vitrification occurred. For the partially photocured samples, the glass transition temperature was raised as the degree of conversion was increased. Most of the measures of the breadth of the glass transition were found to increase with increased conversion for dimethacrylates with short or stiff backbones (TETDMA and bisGMA) while the transition breadth was independent of conversion for either a more flexible dimethacrylate (NEGDMA) or a dimethacrylate network with a lower crosslink density (50 wt % bisGMA/50 wt % PGEMA). This conclusion was generally confirmed by analysis of the viscoelastic parameters in the frequency domain. It is not clear whether these behaviors resulted from differences in the range of molecular motions available in tight networks or if they were due to spatially heterogeneous regions in the network. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 90: 3753–3766, 2003     

Neuroimmune aspects of food intake

There is increasing evidence that diet and the nutrients it contains, may affect the function of the enteric nervous and immune systems, which in turn may alter bacterial diversity and function, intestinal motility and physiology, regulation of inflammation and also affect cognitive brain functions such as memory and even some behaviours.     

Photo-DSC cure kinetics of vinyl ester resins II: influence of diluent concentration

The photopolymerization kinetics of two commercial vinyl ester resins (VERs) and a model VER photoinitiated by the camphorquinone/amine photoinitiator system were monitored using isothermal DSC. A decrease in styrene concentration in model VERs was found to raise the rate of photopolymerization. In contrast, when the styrene was replaced by a monomethacrylate diluent, the photopolymerization rate passed through a maximum near 70 wt% diluent monomer. This difference in the variation of the rate of polymerization with decreased monomer concentration was attributed to the competition of the effects of the higher reactivity of the methacrylyl radical relative to the styryl radical and the lower termination rate for divinyl-rich systems (both of which tend to raise the maximum polymerization rate) and the effects of the reduction in the initiation efficiency and decrease in k_p due to increased fraction of pendant double bonds (which lower the polymerization rate) when the concentration of diluent monomer was reduced. Subsequent dark polymerization was observed during a temperature ramp and the onset of polymerization was independent of resin composition due to vitrification effects during the isothermal photocuring stage. The kinetics during the dark polymerization stage was discussed in terms of the radical concentration and the propagation rate constant. Increases in the concentration of either diluent monomer raised the extent of isothermal cure during the isothermal polymerization because vitrification was delayed by the lower crosslink density and the plasticizing effect of the diluent. Higher levels of diluent also raised the maximally attainable conversion due to reduced topological restrictions for reaction in networks of lower crosslink density.     

Nontechnical problems in knowledge engineering: Implications for project management

Building knowledge-based systems is generally seen as a technical process. While technical difficulties do arise in the course of this process, nontechnical aspects of project development can also be problematic. However, the latter may be harder for technically trained project personnel to recognize as such and solve. This paper addresses some nontechnical issues that can arise in the course of expert systems projects. Some are already well known in the expert systems world; others may not normally be thought of as issues at all, but we urge that they be considered as such. Since all such issues waste time, money, and goodwill, project personnel have reason to identify and avoid them.     

M-ary orthogonal signaling in the presence of Doppler

The performance degradation of an M-ary orthogonal keying (MOK) system due to relative motion between its transmitter and receiver can be minimized by selecting good signaling sets. A formulation for evaluating good signaling sets, or code sets, is developed for Walsh function data modulations. A union bound performance measure which closely approximates the exact probability of a demodulation error, is devised to allow for convenient evaluation of Walsh function codesets. The best Walsh function codesets for codevectors up to length 10 and for M=2, 4, 8 and 16, found by exhaustive search, are presented, along with their performance. In addition, codesets based on error-correcting codes are presented, along with a performance bound expressed in terms of the code's minimum distance