Evaluation of potential anti‐cancer activity of cationic liposomal nanoformulated Lycopodium clavatum in colon cancer cells

Research dealing with early diagnosis and efficient treatment in colon cancer to improve patient''s survival is still under investigation. Chemotherapeutic agent result in high systemic toxicity due to their non‐specific actions on DNA repair and/or cell replication. Traditional medicine such as Lycopodium clavatum (LC) has been claimed to have therapeutic potentials against cancer. The present study focuses on targeted drug delivery of cationic liposomal nanoformulated LC (CL‐LC) in colon cancer cells (HCT15) and comparing the efficacy with an anti‐colon cancer drug, 7‐ethyl‐10‐hydroxy‐camptothecin (SN38) along with its nanoformulated form (CL‐SN38). The colloidal suspension of LC was made using thin film hydration method. The drugs were characterised using ultraviolet, dynamic light scattering, scanning electron microscopy, energy, dispersive X‐ray spectroscopy. In vitro drug release showed kinetics of 49 and 89% of SN38 and LC, whereas CL‐SN38 and CL‐LC showed 73 and 74% of sustained drug release, respectively. Studies on morphological changes, cell viability, cytotoxicity, apoptosis, cancer‐associated gene expression analysis of Bcl‐2, Bax, p53 by real‐time polymerase chain reaction and western blot analysis of Bad and p53 protein were performed. Nanoformulated LC significantly inhibited growth and increased the apoptosis of colon cancer cells indicating its potential anti‐cancer activity against colon cancer cells.Inspec keywords: cancer, biological organs, cellular biophysics, drug delivery systems, drugs, nanomedicine, genetics, DNA, molecular biophysics, biochemistry, lipid bilayers, toxicology, suspensions, colloids, light scattering, X‐ray chemical analysis, solvation, enzymes, nanostructured materialsOther keywords: energy dispersive X‐ray spectroscopy, in vitro drug release, morphological changes, cell viability, cytotoxicity, apoptosis, cancer‐associated gene expression analysis, Bcl‐2, Bax, real‐time polymerase chain reaction, western blot analysis, Bad protein, p53 protein, scanning electron microscopy, dynamic light scattering, ultraviolet scattering, thin film hydration method, colloidal suspension, nanoformulated form CL‐SN38, 7‐ethyl‐10‐hydroxy‐camptothecin, anticolon cancer drug, colon cancer cells HCT15, cationic liposomal nanoformulated LC, targeted drug delivery, therapeutic potentials, Lycopodium clavatum, traditional medicines, cell replication, DNA repair, nonspecific actions, high systemic toxicity, chemotherapeutic agents, patient survival, colon cancer treatment, colon cancer diagnosis, CL‐LC, potential anticancer activity     

Hybridized electronic states between CdSe nanoparticles and conjugated organic ligands: A theoretical and ultrafast photo-excited carrier dynamics study

Formation of densely packed thin films of semiconductor nanocrystals is advantageous for the exploitation of their unique optoelectronic properties for real-world applications. Here we investigate the fundamental role of the structure of the bridging ligand on the optoelectronic properties of the resulting hybrid film. In particular, we considered hybrid films formed using the same CdSe nanocrystals and two organic ligands that have the same bidentate dithiocarbamate binding moiety, but differ in their bridging structures, one bridged by ethylene, the other by phenylene that exhibits conjugation. Based on the results of photo-excited carrier dynamics experiments combined with theoretical calculations on the electronic states of bridged CdSe layers, we show that only the phenylene-based ligand presents a strong hybridization of the molecular HOMO state with CdSe layers, that is a marker of formation of an effective bridge. We argue that this hybridization spread favors the hopping of photo-excited carriers between nanocrystals, which may explain the reported larger photo-currents in phenylene-based hybrid films than those observed in ethylene-based ones.

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Plasmon hybridization engineering in self-organized anisotropic metasurfaces

Nano Research - The engineering of self-organized plasmonic metasurfaces is demonstrated using a maskless technique with defocused ion-beam sputtering and kinetically controlled deposition. The...     

Smooth migration from the GSM system to UMTS for multimedia services

Third generation of mobile systems is now entering the operational phase; European Community ACTS (RACE follow-on) programme just aims at finalizing the plenty of solutions resulting from the European Community RACE programme as well as from several other studies and researches. European manufactures, also deeply involved in the ACTS programme, seem to show a preference for solutions which gradually upgrade the present pan-European GSM successful standard. The underlying concept is the one of asmooth migration from the GSM network to the third generation system, in order to reuse, at least in the first phases of the transition, most of the existing technologies and infrastructures already implemented for the GSM network. In this respect, this paper, by referring to radio interface aspects, proposes a two step evolution: in the first step, a Dynamic Channel Allocation (DCA) strategy with distributed control should be implemented for coping with the high variance of traffic entailed by the reduction of cell dimensions; in the second step, a gradual upgrading of the GSM Base Stations should allow a smooth transition towards the third generation Packet Reservation Multiple Access (PRMA) technique and the provision of broadband services. The paper is partly based upon the work performed by the author in the framework of the RACE project Satellite Integration in the Future Mobile Network (SAINT) and of the European Community ACTS Projects MEDIAN. The opinions herewith reported are not necessarily those of the European Community.     

Excited states of alkali atoms in liquid4He from density functional calculations

Calculations are presented, based on a Density Functional approach, of the first excited states (l=1) of alkali metal atoms (Cs and Na) in liquid ⁴He. We find large departures from the spherical bubble in the liquid⁴He structure when the alkali atom is excited. The shifts from the free atom values of the excitation and emission transition lines are calculated and compared with the experimental results. According to our calculations, the so far unseen radiative deexcitation transition for light alkalis in⁴He may lie in an unexplored infrared region of the spectrum.     

Finite-time control of discrete-time linear systems: Analysis and design conditions

In this paper we deal with some finite-time control problems for discrete-time, time-varying linear systems. First we provide necessary and sufficient conditions for finite-time stability; these conditions require either the computation of the state transition matrix of the system or the solution of a certain difference Lyapunov inequality. Then we address the design problem. The proposed conditions allow us to find output feedback controllers which stabilize the closed loop system in the finite-time sense; all these conditions can be expressed in terms of LMIs and therefore are numerically tractable, as shown in the example included in the paper.     

Enabling energy-efficient and lossy-aware data compression in wireless sensor networks by multi-objective evolutionary optimization

Nodes of wireless sensor networks (WSNs) are typically powered by batteries with a limited capacity. Thus, energy is a primary constraint in the design and deployment of WSNs. Since radio communication is in general the main cause of power consumption, the different techniques proposed in the literature to improve energy efficiency have mainly focused on limiting transmission/reception of data, for instance, by adopting data compression and/or aggregation. The limited resources available in a sensor node demand, however, the development of specifically designed algorithms. To this aim, we propose an approach to perform lossy compression on single node based on a differential pulse code modulation scheme with quantization of the differences between consecutive samples. Since different combinations of the quantization process parameters determine different trade-offs between compression performance and information loss, we exploit a multi-objective evolutionary algorithm to generate a set of combinations of these parameters corresponding to different optimal trade-offs. The user can therefore choose the combination with the most suitable trade-off for the specific application. We tested our lossy compression approach on three datasets collected by real WSNs. We show that our approach can achieve significant compression ratios despite negligible reconstruction errors. Further, we discuss how our approach outperforms LTC, a lossy compression algorithm purposely designed to be embedded in sensor nodes, in terms of compression rate and complexity.