Bacterial cellulose membrane as separation medium

A thin membrane of bacterial cellulose (BC) obtained from Acetobacter culture was tested for its performance as a dialysis membrane in aqueous systems. The BC membrane showed superior mechanical strength to that of a dialysis-grade regenerated cellulose membrane, allowing the use of a thinner membrane than the latter. As a result, the BC membrane gave higher permeation rates for poly(ethylene glycols) as probe solutes. The cutoff molecular weight of the original BC membrane, significantly greater than that of regenerated cellulose, could be modified by concentrated alkali treatments of the membrane. The nature of the change at the ultrastructural level caused by the alkali treatments was studied by X-ray diffraction and scanning electron microscopy. © 1993 John Wiley & Sons, Inc.     

Endomyocardial gene expression during development of pacing tachycardia-induced heart failure in the dog

Selective and specific changes in gene expression characterize the end-stage failing heart. However, the pattern and relation of these changes to evolving systolic and diastolic dysfunction during development of heart failure remains undefined. In the present study, we assessed steady-state levels of mRNAs encoding a group of cardiac proteins during the early development of left ventricular dysfunction in dogs with pacing-induced cardiomyopathy. Corresponding hemodynamic assessments were made in the conscious state in the same animals and at the same time points at baseline, after 1 week of ventricular pacing, and at the onset of clinical heart failure. Systolic dysfunction dominated after 1 week of pacing, whereas diastolic dysfunction was far more pronounced with the onset of heart failure. Atrial natriuretic factor mRNA was undetectable in 7 of 12 hearts at baseline but was expressed in all hearts at 1 week (P < .01 by chi 2 test), and it increased markedly with progression to failure (P = .05). Creatine kinase-B mRNA also rose markedly with heart failure (P < .01). Levels of mRNA encoding beta-myosin heavy chain, mitochondrial creatine kinase, phospholamban, and sarcoplasmic reticulum Ca(2+)-ATPase did not significantly change from baseline, despite development of heart failure. Additional analysis to determine if these mRNA changes were related to the severity of diastolic or systolic dysfunction revealed that phospholamban mRNA decreased in hearts with larger net increases in end-diastolic pressure (+19.2 +/- 1.9 mm Hg) compared with those hearts in which it did not change (+4.0 +/- 4.9, P < .02).(ABSTRACT TRUNCATED AT 250 WORDS)     

Research,technology and development evaluation; developments in Japan

The reseach, technology and development (RTD) evaluation in terms of science and technology policy has come to be important in stimulating research activities and in continuously keeping the vitality and the higher quality of research in RTD institutions. There are two criteria on the RTD evaluation, i.e, in-house evaluation from the stand point of RTD management and independent macroscopic evaluation for the decision making of companies and/or policy making for science and technology policy.The most important point for RTD evaluation in the former criteria is in the mission itself. RTD in universities, public research institutes, and enterprises have different objectives and characteristics. Therefore, the mission and methodology of RTD evaluations should be different, by categorized type and objectives of research institutions, and be developed inhouse. Results of RTD evaluations should be fed back to researchers or engineers and disclosed principally if the mission was to stimulate knowledge creation through RTD activities.The in-house RTD evaluation can be classified in general into three categories: prior evaluation, mid-term review, and ex-post facto review. The methodologies to evaluate RTD in each phase of the RTD process are different, even among those institutes categorized into the same type such as national and regional research institutes. In this paper, two cases of RTD evaluation a) in Riken, which was founded in 1917 as a private research foundation and later reorganized as a semi-public research corporation of the Science and Technology-Agency, b) in regional public research institutes.RTD evaluation from the view point of policy assessment of governmental science and technology policy is discussed through analysis of data obtained by the survey of research activities in regional public research institutes. It can be concluded that developments and introduction of RTD evaluation as a new management system in these institutes is improving the research environment and advancing the quality of research. The differences of RTD evaluation between a Center of Excellence (COE) such as Riken and local technology centers, will be compared and the policy implication of RTD evaluation will also be discussed in terms of promotion of science and technology.     

Deformation kinetics by crazing in glassy polymers

In order to confirm the proposed theory of the plastic deformation caused by crazing, the theoretical deformation curves derived from the dislocation analogue were calculated using the data of craze behaviour measured under static tension and compared with the experimental results of creep and constant strain-rate tension in PMMA and PVC plates wetted by kerosene as a crazing agent. Favourable agreement between the theoretical and experimental results may provide evidence that the proposed method is valuable for estimating the amount of the plastic strain caused by crazing.     

Starch‐based biocomposite films reinforced with cellulose nanocrystals from garlic stalks

The study was conducted to reinforce starch‐based biocomposite films using cellulose nanocrystals (CNCs) from garlic stalks. An average yield of 4.6% by mass based from air‐dried garlic stalks was obtained through alkali delignification, acid hydrolysis and sonication. The isolated CNCs are spherical and have an average diameter of 35 nm and crystallinity of 62%. Fourier transform infrared spectra correspond to the structure of cellulose, but some absorption bands corresponding to hemicelluloses were also noticed. Starch‐based biocomposite films with varying amount of the isolated CNCs as reinforcing filler were prepared by solution casting and evaporation method. Scanning electron micrographs of the films showed homogeneous dispersion of CNC in the starch matrix. Improvement in tensile strength and modulus was at maximum when the starch to CNC ratio is 100:5. The thermal stability of the films, on the other hand, decreased with the addition of CNC. Finally, CNC‐reinforced films had lower moisture uptake than nonreinforced films. POLYM. COMPOS. 34:1325–1332, 2013. © 2013 Society of Plastics Engineers     

Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy

Selective fluorescence excitation of specific molecular species is demonstrated by using coherent control of two-photon excitation with supercontinuum pulses generated with a microstructure fiber. Pulse shaping prior to pulse propagation through the fiber is controlled by a self-learning optimization loop so that the highest fluorescence signal contrast between two fluorescent proteins is obtainable. The self-learning optimization loop successfully controls both the optical nonlinarity of the microstructure fiber and the two-photon excitation of the fluorescent proteins.     

Electrochemical performance for the electro-oxidation of ethylene glycol on a carbon-supported platinum catalyst at intermediate temperature

To determine the kinetic performance of the electro-oxidation of a polyalcohol operating at relatively high temperatures, direct electrochemical oxidation of ethylene glycol on a carbon supported platinum catalyst (Pt/C) was investigated at intermediate temperatures (235–255 °C) using a single cell fabricated with a proton-conducting solid electrolyte, CsH₂PO₄, which has high proton conductivity (>10⁻² S cm⁻¹) in the intermediate temperature region. A high oxidation current density was observed, comparable to that for methanol electro-oxidation and also higher than that for ethanol electro-oxidation. The main products of ethylene glycol electro-oxidation were H₂, CO₂, CO and a small amount of CH₄ formation was also observed. On the other hand, the amounts of C₂ products such as acetaldehyde, acetic acid and glycolaldehyde were quite small and were lower by about two orders of magnitude than the gaseous reaction products. This clearly shows that C–C bond dissociation proceeds almost to completion at intermediate temperatures and the dissociation ratio reached a value above 95%. The present observations and kinetic analysis suggest the effective application of direct alcohol fuel cells operating at intermediate temperatures and indicate the possibility of total oxidation of alcohol fuels.