建筑与交流

作者认为，当今世界每个个人、地域、国家乃至世界之间的关系都更为复杂和多样。此外，这些关系每天都处于动态变化之中。但人们中间仍然存在某种共同的价值观。作者力图使其建筑成为一种超越社会地位、年龄、地区或种族的交流媒介。     

Expression and purification of recombinant human annexin A2 in Pichia pastoris and utility of expression product for detecting annexin A2 antibody

Annexin A2, a Ca2+-dependent phospholipid binding protein, is abundantly expressed in various human organs, which exists as either a membrane-associated, cytosolic or soluble form in serum. We constructed expression systems for recombinant human annexin A2 (rhA2) using Pichia pastoris. The systems are designed to secrete rhA2 as either the N- or C-terminally His6-tagged form to facilitate purification. Both types of rhA2 were overexpressed, but in the N-terminal-truncated form as revealed from the results of N-terminal amino acid sequencing and Western blotting. Therefore, further purification of N-terminally His6-tagged rhA2 was not feasible because of the removal of the N-terminal His6-tag sequence. C-terminally His6-tagged rhA2 was expressed as either a glycosylated or a nonglycosylated form, and the nonglycosylated form was purified using the combination of nickel-immobilized affinity, concanavalin A and cation exchanged column chromatographies. The solid-phase binding of rhA2 was examined by enzyme-linked immunosorbent assay (ELISA), which revealed the specific reactivity of rhA2 against an anti-annexin A2 monoclonal antibody. These results suggest that the expression system using P. pastoris is useful for the preparation of rhA2 that is applicable to the ELISA detection of the anti-annexin A2 antibody.     

Isolation and characterization of murine hepatocytes following collagenase infusion into left ventricle of heart

A method for obtaining mouse hepatocytes by infusing collagenase solution into the left ventricle was established. This technique was shown to be equivalent to the intra-portal infusion method and more practical, especially in postnatal mice with a small body size.     

Rotation‐invariant image matching by geometrical parameter of equidistant edge pixel group

Template matching is a method of searching for a target image area that is similar to a template image. However, the target image area generally must have the right position relative to the template image. Therefore, the computational cost is high when the location and the inclination of the target image area are unknown. This paper proposes a method of searching for a target image area with arbitrary location and an arbitrary inclination by means of geometrical parameters obtained by an equidistant edge group. In this method, the difference of directions is calculated from the edge direction of a referenced edge pixel and from the directions from the referenced edge pixel to other selected edge pixels. After that, matching is performed, using the difference in directions and the matching rates are accumulated. Then the location and inclination of the target image area are sought by scanning the accumulation space. Experimental results show that the proposed method has higher reliability and lower computational cost for image matching. © 2007 Wiley Periodicals, Inc. Electr Eng Jpn, 161(1): 34–42, 2007; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20520     

Evaluation of Models for Spinach Respiratory Metabolism Under Low Oxygen Atmospheres

Anaerobic respiration is a major problem that causes the deterioration of fresh produce packaged under low O₂ atmospheres. The problem becomes more severe and causes high losses in the packages handling at ambient conditions, especially in developing countries. In designing modified atmosphere packaging, the risk of anaerobic development greatly depends upon the accuracy of respiration rate prediction; therefore, the respiration rate model for a particular produce has to be identified. In this study, different atmospheric storage conditions in a closed system were realized to examine the adaptability of respiration rate models for spinach storage under low O₂ at an expected ambient temperature of 25 °C. Six models were applied and it was found that, for aerobic conditions, the respiration rate could be described with a constant respiratory quotient by three models, viz., (a) Michaelis–Menten model without inhibition, (b) Michaelis–Menten model with uncompetitive inhibition, and (c) Langmuir adsorption model, whereas three other models, viz. (d) Michaelis–Menten model with competitive inhibition, (e) Michaelis–Menten model with noncompetitive inhibition, and (f) Michaelis–Menten with mixed inhibition could not be fitted. Among the three successful models, the Michaelis–Menten with uncompetitive inhibition was found to be the most suitable model for practical applications in developing countries where cold-chain systems are lacking. This model can be applied for the prediction of gas composition and optimize the packages, particularly to ensure the aerobic respiration.     

Toward integrated product and process life cycle planning—An environmental perspective

Life cycle engineering (LCE) is a key concept for promoting environmentally sustainable practices among manufacturing firms. A major hurdle in the implementation of LCE is the lack of a systematic and strategic method to design or plan an entire product life cycle. To address this issue, this keynote provides a framework for life cycle development and proposes the concept of life cycle planning. This paper aims to provide explicit and systematic methodologies for life cycle planning by reviewing this research area. Practical cases that employ life cycle planning are also illustrated. Finally, some research directions are suggested.     

Study on intelligent humidity control materials: Water vapor adsorption properties of mesostructured silica derived from amorphous fumed silica

The intelligent humidity control materials were synthesized using fumed silica and quaternary alkylammonium surfactant (decyl-, dodecyl-, tetradecyl- and hexadecyl-trimethylammonium chlorides) as a liquid-crystal template under hydrothermal conditions. X-ray diffraction and transmission electron micrographs indicate that a homogeneous hexagonal structure of SiO2 was formed for those products. The mesostructured products had high B.E.T. surface areas between 960 and 1300 m2/g, with uniform mesopore diameters of 2–4nm. These physical quantities were controlled by varying the size of the organic template. Water vapor adsorption isotherms for these materials possess a sharp increase in adsorption when the relative water vapor pressure is at 40–60%. Over this range of pressures, the maximum amount of adsorbed water content is between 40 and 90%. These silicates have the potential to be effectively used as humidity control elements in construction materials.     

Teacher-directed learning: information-theoretic competitive learning in supervised multi-layered networks

In this paper, we propose a new type of efficient learning method called teacher-directed learning. The method can accept training patterns and correlated teachers, and we need not back-propagate errors between targets and outputs into networks. Information flows always from an input layer to an output layer. In addition, connections to be updated are those from an input layer to the first competitive layer. All other connections can take fixed values. Learning is realized as a competitive process by maximizing information on training patterns and correlated teachers. Because information is maximized, information is compressed into networks in simple ways, which enables us to discover salient features in input patterns. We applied this method to the vertical and horizontal lines detection problem, the analysis of US–Japan trade relations and a fairly complex syntactic analysis system. Experimental results confirmed that teacher information in an input layer forces networks to produce correct answers. In addition, because of maximized information in competitive units, easily interpretable internal representations can be obtained.     

Curing behavior and properties of epoxy resins cured with the diamine having the quinoxaline or triazine structure

2,3-Bis(4-aminophenyl)quinoxaline (1a), 2,3-bis(4-aminophenyl)-6-methylquinoxaline (1b), and 5,6-bis(4-aminophenyl)-3-(2-pyridyl)-1,2,4-triazine (2) were studied as curing agents of epoxy resins. The exotherms on differential scanning calorimetry thermograms of the mixtures of diglycidyl ether of bisphenol A (DGEBA) with 1a, 1b, and 2 were observed at higher temperatures than that of the mixture of DGEBA with a commercially used diamine, for example, 4,4′-diaminodiphenylsulfone (DDS). However, the epoxy resin cured with 1a (EP-1a) showed higher tensile strength to stainless steel at 20°C than that cured with DDS, and the high tensile strength of EP-1a was maintained even at 180°C. The epoxy resin cured with 1b or 2 also possessed higher tensile strength at 20°C than that cured with DDS, but the high tensile strength lowered somewhat at 120°C. Using diamines 1a, 1b, and 2 as a curing agent improved heat distortion temperatures of the cured epoxy resins. © 1998 John Wiley & Sons, Inc. J. Appl. Polym. Sci. 69: 1737–1741, 1998     

集群形态说明

编者按：原文最初以日、英双语形式刊登在日本《JA》1994-4 P248-279,现经作者本人同意、《JA》杂志授权,译为中文在本刊发表。译文校对戎筱（剑桥大学,在读博士生）、巨青（清华大学,研究生）、马明菲（剑桥大学建筑系丘吉尔学院,学生）