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101.
The effect of nerve growth factor (NGF) on ontogenesis of frog mast cells was investigated in vivo by histochemical, morphometric, and ultrastructural analysis. Three groups of tadpoles at various stages of development were used. In the first group, the larvae received i.p. injections of 1 ng NGF/g; the second group received 10 ng NGF/g, while the control group received only the vehicle. The first recognizable mast cells arose symmetrically in the tongue at stage 26 of Witschi's standard table. At stages 26 and 29, the mast cell number in the NGF-injected tadpoles was significantly higher than the control group. From stage 29 onward, the mast cell number rapidly increased in all groups. No significant differences in mast cell number were observed between the control group and the NGF-injected groups at stages 31 and 33. Electron microscopy revealed that at metamorphic climax (stage 33), the mast cells in the NGF-treated groups were more mature than those in the control group. Therefore, nerve growth factor at early stages of tadpole development is likely to induce differentiation of mast cell precursors, while at later stages it is likely to induce maturation of immature mast cells. The close anatomical association between mast cells and perineurium, observed during nerve development, is intriguing. Already in the early stages of nerve development, the mast cells form a network around Schwann cell-axon complexes, together with the perineurial cells. At climax, the mast cells are located between the perineurial layers, suggesting that they may play a role in the tissue-nerve barrier of the perineurium. Nerve growth factor also seems to induce perineurial cell maturation. 相似文献
102.
103.
S. E. Coleman V. I. Nikora S. R. McLean T. M. Clunie B. W. Melville 《Canadian Metallurgical Quarterly》2007,133(2):121-129
Spatial averaging of the Reynolds-averaged Navier–Stokes equations gives the double-averaged Navier–Stokes equations, for which boundary drag appears naturally and explicitly in momentum conservation equations. Increasing use of the double-averaged equations, e.g., for relating flows to three-dimensional bed roughness, for evaluation of profiles of flow stresses and velocities in ecologically significant regions below roughness tops, and for modeling purposes, requires parameterization of boundary drag at subelement scales. Based on seven flows over repeated square-rib roughness and ten flows over repeated fixed simulated sand waves, with measured velocities and bed pressures, expressions for form-drag coefficient CD = f (elevation below roughness top, relative roughness submergence, roughness steepness) are obtained for each of the two-dimensional roughness types. Using these equations, form drag variation with elevation below roughness tops can be calculated using either the double average of the square of local velocity (preferred based on conceptual considerations, trends in coefficient prediction, and also overall drag prediction) or the squared local double-averaged velocity, the roughness area being normal to the flow in each case. Integration of subelement drag given by these expressions is shown to give form-drag coefficient magnitudes and trends for complete individual elements comparable to those obtained by other authors based on measurements or numerical simulations. The ranges of roughness steepness and relative roughness submergence upon which the present equations have been derived need to be noted in consideration of application of the equations. In addition, effective application of the expressions is limited in regions of strongly negative double-averaged velocity. Further work remains to determine drag parameterization for alternative roughness geometries. 相似文献
104.
Liyong Niu Jonathan N. Coleman Hua Zhang Hyeonsuk Shin Manish Chhowalla Zijian Zheng 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(3):272-293
Tremendous efforts have been devoted to the synthesis and application of two‐dimensional (2D) nanomaterials due to their extraordinary and unique properties in electronics, photonics, catalysis, etc., upon exfoliation from their bulk counterparts. One of the greatest challenges that scientists are confronted with is how to produce large quantities of 2D nanomaterials of high quality in a commercially viable way. This review summarizes the state‐of‐the‐art of the production of 2D nanomaterials using liquid‐based direct exfoliation (LBE), a very promising and highly scalable wet approach for synthesizing high quality 2D nanomaterials in mild conditions. LBE is a collection of methods that directly exfoliates bulk layered materials into thin flakes of 2D nanomaterials in liquid media without any, or with a minimum degree of, chemical reactions, so as to maintain the high crystallinity of 2D nanomaterials. Different synthetic methods are categorized in the following, in which material characteristics including dispersion concentration, flake thickness, flake size and some applications are discussed in detail. At the end, we provide an overview of the advantages and disadvantages of such synthetic methods of LBE and propose future perspectives. 相似文献
105.
106.
James P. Coleman Anne T. Lynch Puttanachetty Madhukar John H. Wagenknecht 《Solar Energy Materials & Solar Cells》1999,56(3-4)
We describe herein some of our initial studies in pursuit of a simple, economical method of mass producing electrochromic displays. The approach we have taken is to print the display on polymer film utilizing commercially available conductive inks in an interdigitated electrode structure with a conductive metal oxide powder, dispersed in a polymer binder, as the electrode surface. A range of electrochromic materials suitable for use with an aqueous gel electrolyte have been explored and examples presented. 相似文献
107.
Mari K. N?ss David J. Young Jianqiang Zhang Jan Erik Olsen Gabriella Tranell 《Oxidation of Metals》2012,78(5-6):363-376
Small scale laboratory experiments on the oxidation of liquid silicon have reproduced important features of the industrial refining of liquid silicon: active oxidation led to the formation of amorphous silica spheres as a reaction product. The boundary condition for active oxidation in terms of maximum oxygen partial pressure in the bulk gas was found to lie between 2·10?3 and 5·10?3?atm at T?=?1,500?°C. The active oxidation of liquid silicon had linear kinetics, and the rate was proportional to bulk oxygen partial pressure and the square root of the linear gas flow rate, consistent with viscous flow mass transfer theory. Classical theory for unconstrained flow over a flat plate led to mass transfer rates for SiO(g) which were 2–3 times slower than observed. However, computational fluid dynamic modeling to take into account the effects of reactor tube walls on flow patterns yielded satisfactory agreement with measured volatilization rates. 相似文献
108.
MC Mahon MP Driscoll WJ Glover KM Borchert ZT Kelleher GJ Smith WB Coleman 《Canadian Metallurgical Quarterly》1999,14(2):337-346
We have previously identified and mapped a locus within human chromosome 11p11.2-p12 that suppresses the tumorigenic potential of a rat liver tumor cell line (termed GN6TF) which contains well defined chromosomal aberrations involving rat chromosomes 1, 4, 7, and 10. In the present study, we investigated the potential of this human 11p11.2-p12 liver tumor suppressor locus to suppress the tumorigenic potential of two other rat liver tumor cell lines (GN3TG and GP10TA) following microcell-mediated introduction of human chromosome 11. These tumor cell lines are aneuploid and contain chromosomal abnormalities that are similar to the GN6TF tumor line. The tumorigenic potential and other phenotypic characteristics of GN3TG-11neo and GP10TA-11neo microcell hybrid (MCH) cell lines were variable, and dependent upon the status of the introduced human chromosome 11. MCH cell lines that retained the region of 11p11. 2-p12 delineated by microsatellite markers D11S1385 and D11S903 exhibited suppression of tumorigenicity in vivo (decrease in tumorigenicity and/or elongation of latency), whereas, the tumorigenic potential of one MCH line that lacked markers in this region of human 11p11.2-p12, but retained flanking markers, was not changed from that of the parental tumor cell line. The chromosomal interval between microsatellite markers D11S1385 and D11S903 encompasses the previously localized minimal liver tumor suppressor region, suggesting that a common locus is responsible for tumor suppression among the rat liver tumor cell lines examined. The results of the present study have verified the presence of a liver tumor suppressor locus within human 11p11.2-p12, and have identified a substantial number of microsatellite markers that are closely linked to this tumor suppressor region. These chromosomal markers will facilitate positional cloning of candidate genes from this region, and may prove useful for determining the involvement of this locus in the pathogenesis of human liver cancer. 相似文献
109.
ABSTRACT We have developed a UV-Visible photoacoustic spectrophotometer using relatively inexpensive individual optical components. The instrument was initially developed due to the absence of a commercial photoacoustic instrument in the UV-Visible range. We have found that this custom built instrument allows numerous software generated options that are unavailable on any commercial spectrometric instruments. In addition to recording routine intensity versus wavelength spectra, we can record the following: 1) resolution of the spectra in layered samples; 2) quantitative depth profiles in samples; 3) spectra and spectral fragments can be smoothed and joined into continuous spectra; and 4) signal averaging of spectra can be carried out. The construction of this custom built instrument allowed the maximum flexibility in experimental application with minimum cost to the investigator. 相似文献
110.
Huber A.E. Yeoh T.S. Swint R.B. Woo C.Y. Lee K.E. Roh S.D. Coleman J.J. Faircloth B.O. Zediker M.S. 《Photonics Technology Letters, IEEE》2001,13(10):1064-1066
A new laser design for single-mode high-power applications is reported. The waveguide is a laterally flaring and transversely tapering GaAs buried ridge fabricated by selective area epitaxy. Single-lateral-mode powers of 200 mW were achieved 相似文献