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991.
992.
Taejoon Kang Hongki Kim Jeong Min Lee Hyoban Lee Yun‐Seok Choi Gyeongwon Kang Min‐Kyo Seo Bong Hyun Chung Yongwon Jung Bongsoo Kim 《Small (Weinheim an der Bergstrasse, Germany)》2014,10(20):4200-4206
MicroRNAs (miRNAs) are emerging new biomarkers for many human diseases. To fully employ miRNAs as biomarkers for clinical diagnosis, it is most desirable to accurately determine the expression patterns of miRNAs. The optimum miRNA profiling method would feature 1) highest sensitivity with a wide dynamic range for accurate expression patterns, 2) supreme specificity to discriminate single nucleotide polymorphisms (SNPs), and 3) simple sensing processes to minimize measurement variation. Here, an ultra‐specific detection method of miRNAs with zeptomole sensitivity is reported by applying bi‐temperature hybridizations on single‐crystalline plasmonic nanowire interstice (PNI) sensors. This method shows near‐perfect accuracy of SNPs and a very low detection limit of 100 am (50 zeptomole) without any amplification or labeling steps. Furthermore, multiplex sensing capability and wide dynamic ranges (100 am –100 pm ) of this method allows reliable observation of the expression patterns of miRNAs extracted from human tissues. The PNI sensor offers combination of ultra‐specificity and zeptomole sensitivity while requiring two steps of hybridization between short oligonucleotides, which could present the best set of features for optimum miRNA sensing method. 相似文献
993.
994.
Seong-Ho Baek Bum-Young Noh Jang-Kyoo Shin Jae Hyun Kim 《Journal of Materials Science》2012,47(9):4138-4145
We introduce a new type of silicon micro-wire (SiMW) solar cell with a conformal zinc oxide (ZnO) nanorods anti-reflection
coating (ARC) and discuss the optical and photovoltaic properties of the SiMW solar cells with controlled ZnO nanorods. The
fabrication processes were composed of metal-assisted electroless etching combined with photolithography, spin-on-dopant diffusion,
and hydrothermal synthesized ZnO nanorods growth. We found that the combination of Si wire geometry and ZnO ARC was able to
maximize the light absorption and to minimize the light reflectance. Illuminated current–voltage (I–V) results show that the photovoltaic efficiency of SiMW solar cells with optimized ZnO ARC was enhanced more than 50% and
the short-circuit current density was improved by over 43% compared to SiMW solar cells without ZnO ARC. This is mainly attributed
to the reduced light reflectance and enhanced photon absorption. These hybrid structures are promising for making low-cost
Si wire solar cells and making them applicable to photovoltaic devices with large areas. 相似文献
995.
996.
We present the effects on the thermal annealing of silicon quantum dots (Si QDs) embedded in silicon nitride. The improved photoluminescence (PL) intensities and the red-shifted PL spectra were obtained with annealing treatment in the range of 700 to 1000 °C. The shifts of PL spectra were attributed to the increase in the size of Si QDs. The improvement of the PL intensities was also attributed to the reduction of point defects at Si QD/silicon nitride interface and in the silicon nitride due to hydrogen passivation effects. 相似文献
997.
In this study, solution-processed nickel oxide (NiO) thin film was investigated as a hole transport layer on anode to improve the performance of bulk heterojunction solar cell based on poly(3-hexylthiophene) (P3HT) and [6,6]-phenyl C61-butyric acid methyl ester (PCBM). We fabricated NiO thin film without any vacuum-related process. Characterization of the NiO film under this study shows that it has maximum transmittance of 93.22% and bandgap of 3.84 eV which are proper for solar cell. Insertion of the NiO layer affords to realize enhanced power conversion efficiency of 1.97% and fill factor of 52.11% showing improvement over existing cells. In addition, NiO suggests one solution of minimizing conventional problems of poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) such as interfacial power losses, corrosion of indium tin oxide layer, and degradation of the devices. The value of such hole transporting and electron blocking properties is clearly demonstrated and could be applicable to other organic photovoltaics. 相似文献
998.
Herman I Yeo J Hong S Lee D Nam KH Choi JH Hong WH Lee D Grigoropoulos CP Ko SH 《Nanotechnology》2012,23(19):194005
In this paper we have demonstrated the simple, low cost, low temperature, hydrothermal growth of weeping willow ZnO nano-trees with very long branches to realize high efficiency dye-sensitized solar cells (DSSCs). We also discuss the effects of branching on solar cell efficiency. By introducing branched growth on the backbone ZnO nanowires (NWs), the short circuit current density and the overall light conversion efficiency of the branched ZnO NW DSSCs increased to almost four times that for vertically grown ZnO NWs. The efficiency increase is attributed to the increase in surface area for higher dye loading and light harvesting and also to reduced charge recombination through direct conduction along the crystalline ZnO branches. As the length of the branches increased, the branches became flaccid and the increase in solar cell efficiency slowed down because the effective surface area increase was hindered by branch bundling during the drying process and subsequent decrease in the dye loading. 相似文献
999.
An approach for the characterization of glycosylation sites and oligosaccharide heterogeneity in glycoproteins based on a combination of nonspecific proteolysis, deglycosylation, and matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FT MS) is described. Glycoproteins were digested with Pronase yielding primarily glycopeptides and amino acids. Nonglycosylated peptide fragments were susceptible to complete Pronase digestion to their constituent amino acids. Steric hindrance prohibited the digestion of the peptide moiety attached to the glycan. Glycopeptides were desalted and concentrated using solid-phase extraction and analyzed by MALDI MS. The oligosaccharides were also analyzed by MALDI MS after releasing the glycans from glycoproteins using PNGase F. The peptide moiety of the glycopeptides was identified by subtracting the masses of the glycans derived from PNGase F treatment from the masses of the glycopeptides. The experimental strategy was validated using glycoproteins with known oligosaccharide structures, ribonuclease B and chicken ovalbumin. This procedure was then used to determine the N-glycosylation sites and site heterogeneity of a glycoprotein whose glycosylation pattern was unknown, namely, the Xenopus laevis egg cortical granule lectin. This procedure is useful for determining protein site heterogeneity and structural heterogeneities of the oligosaccharide moiety of glycoproteins. 相似文献
1000.
Jung YK Kim TW Jung C Cho DY Park HG 《Small (Weinheim an der Bergstrasse, Germany)》2008,4(10):1778-1784
A micropatterned polydiacetylene (PDA) chip, utilizing the unique fluorogenic property of PDA and a specific biotin-streptavidin (STA) interaction, is constructed to detect pathogen infections. To construct the PDA chip, biotin-modified diacetylene liposomes are immobilized on aldehyde glass and conjugated with STA, followed by UV irradiation to polymerize the STA-functionalized diacetylene liposomes. Genomic DNA of a model pathogen, Chlamydia trachomatis, is isolated from human samples and biotin-labeled target DNA is obtained through PCR amplification using biotin-11-dUTP. Owing to the stimulus caused by the biotin-STA interaction, the biotinylated DNA induces an intense fluorescence signal on the immobilized PDA. By using this strategy, it is possible to diagnose Chlamydia infections by applying DNA samples from several nonhealthy humans to a single PDA chip. The results of this study serve as the basis for a new strategy for fluorogenic PDA microarray-based diagnosis of pathogen infections. 相似文献