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21.
Cíntia Reis da Silva Marilia Lordelo Cardoso Silva Helio Mitoshi Kamida Aristoteles Goes‐Neto Maria Gabriela Bello Koblitz 《Food Science & Nutrition》2014,2(4):299-307
Lytic enzymes are widely used in industrial biotechnology as they are able to hydrolyze the bacterial cell wall. One application of these enzymes is the clarification of the culture broth for the production of xanthan gum, because of its viability in viscous media and high specificity. The screening process for filamentous fungi producing lytic enzymes, the optimization of production of these enzymes by the selected microorganism, and the optimization of the application of the enzymes produced in the clarification of culture broth are presented in this article. Eleven fungal isolates were tested for their ability to produce enzymes able to increase the transmittance of the culture broth containing cells of Xanthomonas campestris. To optimize the secretion of lytic enzymes by the selected microorganism the following variables were tested: solid substrate, initial pH, incubation temperature, and addition of inducer (gelatin). Thereafter, secretion of the enzymes over time of incubation was assessed. To optimize the clarification process a central composite rotational design was applied in which the pH of the reaction medium, the dilution of the broth, and the reaction temperature were evaluated. The isolate identified as Aspergillus tamarii was selected for increasing the transmittance of the broth from 2.1% to 54.8%. The best conditions for cultivation of this microorganism were: use of coconut husk as solid substrate, with 90% moisture, at 30°C for 20 days. The lytic enzymes produced thereby were able to increase the transmittance of the culture broth from 2.1% to 70.6% at 65°C, without dilution and without pH adjustment. 相似文献
22.
Bart Goes Javier Gil-Sevillano Urbain DHaene 《International Journal of Mechanical Sciences》1999,41(9):1031
During coiling operations high residual stresses are frequently developed in steel wire. In this paper the stress distribution in wires during coiling, unwinding and subsequent tensile testing is modelled for numerous bending degrees, assuming perfect Voce plastic deformation and linear elastic behaviour. The influence of such residual stresses on the observed tensile test data can be deduced. It is shown that coiling with spool radii as used today industrially can lead to measurement of wire properties deviating significantly from the “true” properties of a properly coiled wire. Also, a method is proposed to deduce the original flow behaviour of coiled samples from tensile test curves, hence filtering the effect of the residual stresses. 相似文献
23.
M Hobart V Ramassar N Goes J Urmson PF Halloran 《Canadian Metallurgical Quarterly》1996,62(12):1895-1901
24.
Elck Almeida Carvalho Laís Vieira Nunes Laís Mendes dos Santos Goes Erik Galvão Paranhos da Silva Marcelo Franco Eduardo Gross 《Chemical Engineering Communications》2018,205(7):975-985
This work investigated the xylanase production by fungi isolates from tropical agroforestry systems using peach-palm industrial waste as a substrate. Trichoderma stromaticum AM7 was the best xylanase producer and there was a 160% increase in xylanase activity after optimizing by the Box–Behnken statistical design. The optimization process demonstrated that the maximum activity occurred at 0.95% nitrogen concentration after 6 days of cultivation at 32°C, which achieved a yield of 1440?U?g?1 of dry initial substrate. Analysis by scanning electron microscopy showed degradation of the fibers after cultivation. The optimum pH and temperature for xylanase activity were 5.0 and 50°C, respectively. The extensive degradation of the peach-palm waste and xylanase production by T. stromaticum AM7 was due to the combination of a good physicochemical composition of the culture medium and the characteristics of the selected fungus. 相似文献
25.
A mobile communication system for high-speed thruways is currently under consideration in Japan. The system consists of fixed stations installed at regular intervals (2-5 km) along the thruway which are connected to a host computer. Subscribing customers can communicate with the system as they drive through each station's communication zone of minimal type (40-100 m). The authors develop and analyze a stochastic model for this new communication system. The model closely follows the sequence of operations which actually take place according to a communication protocol for handling collisions, and requires no exponential assumptions for the underlying service times. The tradeoff between the total number of stations installed and the probability of successful completion of a task within z km after the initial submission is investigated. Some numerical results are also given for illustrating the tradeoff, with validation via SIMAN simulation 相似文献
26.
Frank M. L. van der Goes Gerard C. M. Meijer 《Analog Integrated Circuits and Signal Processing》1997,14(3):249-260
This paper presents a new universal transducer interface.This interface, which is read out by a microcontroller, servicesthe following sensor elements: capacitors, platinum resistors,thermistors, resistive bridges and potentiometers. The A/D conversionis based on a first-order oscillator. A combination of classicaland some new measurement techniques has been applied on a singlechip to obtain high accuracy, good long-term stability and areduction of the effects of interference and parasitic elements.The circuit has been fabricated in a 0.7 µm CMOSprocess. The main test results are: an accuracy of 10–15bits and a resolution up to 16 bits while the measurement timeis in the range 1–100ms. These results hold over the temperaturerange –20°C to 80°C. Calibrationof the electronic part is not required. The number of externalcomponents has been kept to a minimum. 相似文献
27.
In today’s dynamic business environments, organizations are under pressure to modernize their existing software systems in order to respond to changing business demands. Service oriented architectures provide a composition framework to create new business functionalities from autonomous building blocks called services, enabling organizations to quickly adapt to changing conditions and requirements. Characteristics of services offer the promise of leveraging the value of enterprise systems through source code reuse. In this respect, existing system components can be used as the foundation of newly created services. However, one problem to overcome is the lack of business semantics to support the reuse of existing source code. Without sufficient semantic knowledge about the code in the context of business functionality, it would be impossible to utilize source code components in services development. In this paper, we present an automated approach to enrich source code components with business semantics. Our approach is based on the idea that the gap between the two ends of an enterprise system—(1) services as processes and (2) source code—can be bridged via similarity of data definitions used in both ends. We evaluate our approach in the framework of a commercial enterprise systems application. Initial results indicate that the proposed approach is useful for annotating source code components with business specific knowledge. 相似文献
28.
29.
Nuno Pereira João Goes 《International Journal of Circuit Theory and Applications》2020,48(7):1093-1107
In the field of radio receivers, downconversion methods usually rely on one (or more) explicit mixing stage(s) before the analog-to-digital converter (ADC). These stages not only contribute to the overall power consumption but also have an impact on area and can compromise the converter's performance in terms of noise and linearity. As an alternative, we propose a receiver architecture that considers the ADC as both a quantizer and a downconverter block. This is achieved through the use of a variable reference signal (in this case, a voltage), as opposed to classic time-invariant reference signals. When embedded into a charge-sharing (CS) successive approximation register (SAR) ADC, this varying reference voltage is “saved” in the digital-to-analog converter (DAC) capacitor bank during the sampling phase, preventing any conversion errors. Furthermore, a phase-locked loop (PLL) is used in order to provide an on-chip solution for the generation of this variable reference voltage, which also removes the need for dedicated bandgap circuits and reference buffers. Post-layout simulations of an 8-bit 50 MS/s CS-SAR ADC show that the proposed “embedded mixing” technique is able to downconvert a high-frequency signal while also increasing the effective resolution by around 0.5 bits, when compared with a standard DC reference voltage. 相似文献
30.
Julie Digne David Cohen-Steiner Pierre Alliez Fernando de Goes Mathieu Desbrun 《Journal of Mathematical Imaging and Vision》2014,48(2):369-382
We introduce a robust and feature-capturing surface reconstruction and simplification method that turns an input point set into a low triangle-count simplicial complex. Our approach starts with a (possibly non-manifold) simplicial complex filtered from a 3D Delaunay triangulation of the input points. This initial approximation is iteratively simplified based on an error metric that measures, through optimal transport, the distance between the input points and the current simplicial complex—both seen as mass distributions. Our approach is shown to exhibit both robustness to noise and outliers, as well as preservation of sharp features and boundaries. Our new feature-sensitive metric between point sets and triangle meshes can also be used as a post-processing tool that, from the smooth output of a reconstruction method, recovers sharp features and boundaries present in the initial point set. 相似文献