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81.
Analysis of the Fourier components of through-focal images in scanning transmission electron microscopy with a high angle annular dark field detector is used to assess illumination defocus values. The method is based on a least squares fitting of the peculiar dependence of Fourier components of the high angle annular dark field image on defocus. The validity of the method has been checked against simulations and experiments obtaining a good level of accuracy on the defocus measurement (δf=2 nm) for simulated specimen thickness up to 40 nm. The difference between simulated and experimental Fourier coefficients for large defoci can be used to estimate the specimen thickness at least up to 30 nm but with decreasing precision for larger thickness. 相似文献
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84.
In the present article we offer information regarding the estimated amounts of cadmium which may be consumed by 12,000 students 12 to 17 years old, who attend boarding secondary schools. The method of analysis used to determine cadmium is that reported by the Association of Official Analytical Chemists using atomic absorption spectrometry with air-acetylene flame. The estimated quantities of cadmium which these students may consume according to the offered foods are smaller than those recommended as provisional tolerable weekly intake by the Joint FAO/WHO Expert Committee on Food Additives. 相似文献
85.
S González C Grillo AG De Nicola G Piroli J Angulo BS McEwen AF De Nicola 《Canadian Metallurgical Quarterly》1994,63(5):1962-1970
The Na+,K(+)-ATPase plays a key role in the regulation of ion fluxes and membrane repolarization in the CNS. We have studied glucocorticoid effects on biosynthesis of the Na+,K(+)-ATPase and on ouabain binding in the ventral horn of the spinal cord using intact rats, adrenalectomized (ADX) rats, and ADX rats receiving dexamethasone (ADX+DEX) during 4 days. Cryostat sections from spinal cords were incubated with a 35S-oligonucleotide coding for the alpha 3-subunit or a 3H-cDNA coding for the beta 1-subunit of the Na+,K(+)-ATPase using in situ hybridization techniques. In ventral horn motoneurons, grain density per cell and grain density per area of soma for both probes were slightly reduced in ADX rats but significantly increased in the ADX+DEX group, using ANOVA and the Bonferroni's test. Statistical analysis of frequency histograms of neuronal densities further indicated a significant shift to the right for intact rats compared with ADX rats for both probes. Concomitantly, [3H]ouabain binding to membrane preparations from ventral horns was reduced in ADX rats and restored to normal by DEX administration. No effect of adrenalectomy or DEX treatment was obtained in the dorsal horn. In conclusion, glucocorticoids positively modulate the mRNA for the alpha 3-subunit and the beta 1-subunit of the Na+,K(+)-ATPase and recover ouabain binding to normal values. The increments of the synthesis and activity of an enzyme affecting membrane repolarization and synaptic neurotransmission are consistent with the alleged stimulatory effect of glucocorticoids on spinal cord function. 相似文献
86.
Lorenza Pastorino Federica Grillo Manuela Albertelli Paola Ghiorzo William Bruno 《International journal of molecular sciences》2021,22(19)
Genomic studies have identified some of the most relevant genetic players in Neuroendocrine Neoplasm (NEN) tumorigenesis. However, we are still far from being able to draw a model that encompasses their heterogeneity, elucidates the different biological effects consequent to the identified molecular events, or incorporates extensive knowledge of molecular biomarkers and therapeutic targets. Here, we reviewed recent insights in NEN tumorigenesis from selected basic research studies on animal models, highlighting novel players in the intergenic cooperation and peculiar mechanisms including splicing dysregulation, chromatin stability, or cell dedifferentiation. Furthermore, models of tumorigenesis based on composite interactions other than a linear progression of events are proposed, exemplified by the involvement in NEN tumorigenesis of genes regulating complex functions, such as MEN1 or DAXX. Although limited by interspecies differences, animal models have proved helpful for the more in-depth study of every facet of tumorigenesis, showing that the identification of driver mutations is only one of the many necessary steps and that other mechanisms are worth investigating. 相似文献
87.
JD Mitchell DJ Mathisen CD Wright JC Wain DM Donahue AC Moncure HC Grillo 《Canadian Metallurgical Quarterly》1999,117(1):39-52; discussion 52-3
88.
Ronaldo S. de Biasi Maria L. N. Grillo 《Journal of the American Ceramic Society》2009,92(5):1139-1140
The electron magnetic resonance (EMR) technique was used to investigate the diffusion of gadolinium in ceria (CeO2 ) powders. The EMR absorption intensity was measured for several annealing times and three different temperatures of isothermal annealing: 1273, 1323, and 1373 K. The activation temperature for diffusion, calculated from the experimental data using a theoretical model based on the Fick equation, was found to be E A =485±5 kJ/mol. This value is very close to the one obtained for the diffusion of gadolinium in an oxide with a similar crystal structure, UO2 , using the secondary ion mass spectrometry technique, E A =492 kJ/mol. On the other hand, it is much larger than the one obtained for the diffusion of gadolinium in yttrium vanadate (YVO4 ), E A =177 kJ/mol, a compound with a different crystal structure. 相似文献
89.
Alfio Grillo Michael Lampe Gabriel Wittum 《Computing and Visualization in Science》2010,13(6):287-297
We provide three-dimensional numerical simulations of the thermohaline-driven buoyancy of a brine “parcel” immersed in an
initially homogeneous porous medium of hydrological interest. Our purpose is to improve our understanding of the thermohaline
flow through the 3D visualization of the evolving patterns generated by the distributions of brine, temperature, and fluid
density in the porous medium. We propose a possible physical interpretation of our results, which are obtained within the
approximations usually employed in the context of density- and temperature-driven flow. 相似文献
90.
Ciminiello P Dell'Aversano C Fattorusso E Forino M Magno GS Tartaglione L Grillo C Melchiorre N 《Analytical chemistry》2006,78(17):6153-6159
A new method for sensitive, specific, and direct determination of palytoxin is proposed herein. It is based on combination of reversed-phase liquid chromatography with mass spectrometry (LC-MS). The new method was set up on a turbo ion spray-triple quadrupole MS instrument operating in selected ion monitoring (SIM) and multiple reaction monitoring (MRM) acquisition modes (positive ions). The minimum detection levels for matrix-free toxin on column were thus estimated from the data to be 200 and 125 pg in SIM and MRM modes, respectively. Spiking experiments before and after extraction allowed us to assess limits of detection and quantitation for palytoxin in matrix, accuracy, and intraday and interday reproducibility of the method. The developed method was decisive for the analysis of a plankton sample collected along Genoa coasts in July 2005 when respiratory illness in people exposed to marine aerosols occurred. It is suggested that putative palytoxin was the causative agent responsible for patients' symptoms and demonstrated for the first time the presence of such a toxin in Italian waters. 相似文献