Introduction of internal cysteines as conformational probes in yeast phosphoglycerate kinase

Several mutants of yeast phosphoglycerate kinase, each containingonly one internal cysteine residue, were constructed from asingle mutant devoid of cysteine. These cysteines were introducedas local conformational probes in selected buried positions.The enzyme activity, conformational characteristics and stabilityindicated that the mutations introduced only small perturbationsin the molecule. The folding–unfolding process mediatedby guanidine hydrochloride under equilibrium conditions wasstudied by following the variations in ellipticity and the reactivityof the cysteine residue towards 5,5'-dithiobis(nitrobenzoate).The process was found to be reversible except for mutant C97A,V49C,suggesting that this region located in helix I might be crucialin determining an intermediate on the folding pathway. The transitionsobtained by the two signals did not coincide, indicating thatthe local structures, in several parts inside the molecule,are more sensitive to the denaturant than the overall conformation.     

浅谈南方住宅建筑节能

以可持续发展的观点，审视南方地区住宅建设，建筑节能滞后于建设发展，当引起定足够重视。应提高节能意识，按照地区气候特点，有针对性地采取有效措施以实现建筑节能。强调对自然条件的充分利用；提高围护结构热工性能，采用较高档的节能技术与产品；外窗是夏季隔热最薄弱的环节；活动式外遮阳的效果最为显著；空调设备应选 用能效比较良好的产品等。评价南方地区住宅建筑节能的最终效果是建筑物耗冷量指标等，尚待专门研究。     

GPS信息在手持终端设备上的应用实现

GPS由于其独特的应用性，现在已经逐渐深入人们的日常生活。由此，我们介绍了一种实现GPS应用的具体方法，主要内容包括硬件的基本原理，GPS模块的介绍，GPS信息数据的接收处理，重点讲解了GPS与地理信息系统GIS(Geographic Information System)坐标之间的转换算法。该设计方法的正确性在手持信息终端设备上得到了验证。     

夏热冬冷地区农村节能住宅设计思考

随着社会和经济的发展,住宅节能设计的益处已经逐步被公众所认同,节能设计其本身也在各大中城市被广泛的推广和采用。但与此相对,在广大农村地区,对于住宅节能方面的考虑却仍处于起步阶段,有些地方甚至还是一片空白。正是在这种情况下,针对夏热冬冷地区的气候特点以及该地区现在农村村宅的现状,提出了适宜农村村宅的相关节能措施,其中包括：①节能与节地相结合;②节能与节约相结合;③节能与营造健康住宅相结合。同时,在最后还对在夏热冬冷地区进行农村节能住宅设计提出了相关的建议。     

钢结构中高强度螺栓连接形式相关问题的探讨

对摩擦型和承压型连接的高强度螺栓受力特征和应用范围进行对比分析,并对高强度螺栓连接在设计应用过程中存在的某些问题及影响因素进行探讨,给出钢结构高强螺栓连接设计时有价值的建议。     

流体动力式空调喷水室理论及靶式撞击流喷嘴的实验研究

介绍了流体动力式空调喷水室的理论及撞击流技术应用现状，系统阐述了撞击流喷嘴的雾化机理。对改进后的靶式撞击流喷嘴进行了实验研究。     

Effects of steeping and anaerobic treatment on GABA (γ-aminobutyric acid) content in germinated waxy hull-less barley

Effects of steeping conditions (time, temperature and soaking solution) and anaerobic storage on the gamma-aminobutyric acid (GABA) content in waxy hull-less barley grains during germination was examined. The barley kernel was steeped for 16 h at different temperatures (5, 15 or 35 °C) either in water or in a buffer solution (pH 6.0, 50 mmol/L sodium acetate) and then germinated at 15 °C for 72 h. To reach the optimum water content (36–44 g/100 g) for germination, a longer steeping period was required when steeping temperature was lower (16 h at 5 °C vs. 8 h at 15 °C). At 35 °C for steeping, however, the water content in the grains increased excessively, and thus germination percentage became much less than those at 5 and 15 °C. The GABA content increased with increasing germination time and was higher in the buffer solution than water. These findings indicate that the glutamate decarboxylase (GAD), which is the rate-limiting enzyme for GABA synthesis, is more activated by extending germination at controlled pH (6.0). An anaerobic storage with nitrogen in the dark for the germinated barley grains substantially raised the GABA content: 14.3 mg/100 g after the treatment for 12 h, which was four times higher than that of control sample (3.7 mg/100 g). Overall results suggest that the steeping prior to germination greatly affects the GABA production during the germination of barley, and the anoxia storage with nitrogen after the germination increases the GABA content.     

棉籽蛋白粉的生物脱毒研究

以选育的ＦＢ－２－８菌株［１］,在５４℃±１℃按１０％接种量,将棉籽蛋白粉进行固态厌氧发酵４８ｈ。结果：棉籽蛋白粉中总棉酚被脱毒８０％～９０％,游离棉酚含量＜０．０１％,粗蛋白含量无明显变化,蛋白质中各氨基酸的比例未变。     

微生物浸出难浸黄铜矿的研究

比较详细地介绍了国内外微生物浸出黄铜矿及其多金属的最新成果,以期对我国微生物浸出铜工业的发展有一定的指导作用