Local Epidermal Endocrine Estrogen Protects Human Melanocytes against Oxidative Stress,a Novel Insight into Vitiligo Pathology

As the outermost barrier of the body, skin is a major target of oxidative stress. In the brain, estrogen has been reported synthesized locally and protects neurons from oxidative stress. Here, we explored whether estrogen is also locally synthesized in the skin to protect from oxidative stress and whether aberrant local estrogen synthesis is involved in skin disorders. Enzymes and estrogen receptor expression in skin cells were examined first by quantitative real-time PCR and Western blot analyses. Interestingly, the estrogen synthesis enzyme was mainly localized in epidermal keratinocytes and estrogen receptors were mainly expressed in melanocytes among 13 kinds of cultured human skin cells. The most abundant estrogen synthesis enzyme expressed in the epidermis was 17β-hydroxysteroid dehydrogenase 1 (HSD17β1) localized in keratinocytes, and the most dominant estrogen receptor expressed in the epidermis was G protein-coupled estrogen receptor 1 (GPER1) in melanocytes. To investigate whether keratinocyte-derived estradiol could protect melanocytes from oxidative stress, cultured human primary epidermal melanocytes (HEMn-MPs) were treated with H₂O₂ in the presence or absence of 17β estradiol or co-cultured with HSD17β1 siRNA-transfected keratinocytes. Keratinocyte-derived estradiol exhibited protective effects against H₂O₂-induced cell death. Further, reduced expression of HSD17β1 in the epidermis of skin from vitiligo patients was observed compared to the skin from healthy donors or in the normal portions of the skin in vitiligo patients. Our results suggest a possible new target for interventions that may be used in combination with current therapies for patients with vitiligo.     

Senescence Marker Protein 30 (SMP30): A Novel Pan-Species Diagnostic Marker for the Histopathological Diagnosis of Breast Cancer in Humans and Animals

Senescence marker protein 30 (SMP30) is a cell survival factor playing an important role in vitamin C synthesis and antiapoptosis. Moreover, its cytoprotective role suggests a possibility to be related to cancer cell survival. Mammary carcinoma is a common cancer in both humans and animals. Because of its histopathological diversity, especially in the early stage, histopathological diagnosis may be complicated; therefore, a diagnostic marker is helpful for confirmation. The present study analyzed the expression pattern of SMP30 in mammary carcinoma in humans, dogs, and cats. Immunohistochemistry, immunofluorescence, and western blot analysis were used to investigate SMP30 expression patterns. The expression was specifically observed in neoplastic glandular epithelial cells. The expression increased with the malignancy of glandular epithelial cells with a highly proliferative status. However, SMP30 expression was low in normal mammary gland tissues or well-differentiated adenoma tissues. The patterns were consistently reproduced in canine primary mammary carcinoma cells and MCF-7 and MDA-MB-231 human carcinoma cell lines. This study provides useful information to understand SMP30 expression in various stages of mammary carcinoma and to suggest its utility as a pan-species diagnostic marker, thereby helping to establish strategies for diagnosing mammary carcinoma in several species.     

Operational optimization of a building-level integrated energy system considering additional potential benefits of energy storage

As a key component of an integrated energy system (IES), energy storage can effectively alleviate the problem of the times between energy production and consumption. Exploiting the benefits of energy storage can improve the competitiveness of multi-energy systems. This paper proposes a method for day-ahead operation optimization of a building-level integrated energy system (BIES) considering additional potential benefits of energy storage. Based on the characteristics of peak-shaving and valley-filling of energy storage, and further consideration of the changes in the system’s load and real-time electricity price, a model of additional potential benefits of energy storage is developed. Aiming at the lowest total operating cost, a bi-level optimal operational model for day-ahead operation of BIES is developed. A case analysis of different dispatch strategies verifies that the addition of the proposed battery scheduling strategy improves economic operation. The results demonstrate that the model can exploit energy storage’s potential, further optimize the power output of BIES and reduce the economic cost.     

基于报废量预测的电子废弃物回收产业现状与对策 —— 以长三角为例

长三角为我国经济最活跃、电子废弃物回收产业发展相对完善的地区之一。运用stata多元回归分析报废量的影响因素,用Holt模型预测长三角区域电子废弃物报废量。结果显示, 2050年电视机累计报废量将达到35亿台,手机报废量将达到65亿部。通过分析长三角电子废弃物回收产业规模、回收体系、地方性法律政策及公共意识4个方面,发现在回收体系构建方面,仍然存在非正规回收渠道占据主要市场,正规企业产能过剩以及回收点覆盖不合理等问题。结合未来电子废弃物报废量与回收产业现状,提出了系统性的政策建议。     

渤海注聚井区油井深部解堵工艺研究及应用

针对渤海油田油井解堵效果变差、有效期降低等问题,提出了扩大解堵半径、优选解堵剂体系、强化解堵效果的解堵思路。通过工艺研究、工艺优选,形成了深穿透解堵技术。深穿透解堵技术主要包含基质解堵、降阻造缝和储层深部解堵三个过程。对深穿透解堵工艺机理进行了研究,并形成了一套现场可应用的工艺技术。针对锦州油田注聚井区堵塞问题,从聚合物溶液降黏、模拟含聚堵塞物、现场含聚堵塞物降解三个方面优选出一种复合解堵剂,结合工艺思路形成施工方案,并进行现场应用,取得了较好的增液、增油效果。     

新工科背景下安全工程专业创新型人才培养路径研究 #br#

为实现新工科背景下安全工程专业创新人才培养目标,基于对创新能力内涵的充分理解,从创新精神、创新意识和创新思维三重角度出发,充分利用“校企协同”育人优势,确定安全工程专业创新型人才培养路径,包括创新型课程体系建设、创新型多维实践平台建设、创新型师资队伍建设及多学科交叉创业活动4条路径。将创新能力培养贯穿于安全工程专业人才培养的全过程,为高校实现创新型人才培养提出建议和参考。     

欠驱动异构式下肢康复机器人动力学分析及参数优化

针对现有外骨骼机器人人机自由度不匹配和关节对中性差的问题,提出欠驱动下肢康复机器人. 欠驱动机器人只有4个直线驱动,驱动的直线运动通过推杆和人机连接机构转化为人下肢在矢状面内的屈伸运动,带动人体进行步态康复训练. 建立机器人系统的人机耦合模型,进行模型的动力学分析,对人机耦合模型中影响动力学结果的参数进行分析,建立驱动力与肢体推动力之间的关系模型,并以推力系数最大为目标进行参数分析与优化,得到最佳的结构参数. 根据优化后的结构参数搭建康复机器人实验系统,对髋、膝关节驱动力与角度进行对比. 实验结果表明最大髋关节角度误差为2.9°,最大膝关节角度误差为6.4°,最大误差均约为9%,验证了动力学模型和参数优化结果的正确性.     

强度传输方程和神经网络融合的三维重构算法

针对原有强度传输方程法所恢复的相位精度不够精确的缺点,提出强度传输方程和神经网络融合的三维重构算法. 利用强度传输方程求解出物体不同角度的初始相位,利用神经网络算法进行优化,根据不同角度的最终恢复相位结合乘法技术重构出三维体信息. 该算法具有精度高的特点,可以为三维成像技术的应用提供参考. 对于实验中的示例图像,该算法将强度传输方程得到的相位误差从21.40%降低为5.26%,重构三维物体与模拟真实物体的相关程度为显著相关.     

"双高计划"背景下校企专兼结合"双师型"教师团队建设研究

"双师型"教师团队建设是"双高计划"实施过程中最基础、最重要的环节.现阶段"双高"院校急需建设完善适合我国国情的"双师型"教师团队.分析新时代"双高计划"背景下"双师型"教师团队建设的特点、意义以及面临的问题,提出4项推进措施,旨在促进"双高"院校构建适应"双高计划"的新型校企专兼结合的"双师型"教师团队,创新师资团队建设机制,形成高职教育与企业培养共同发展的运行模式.     

基于深度神经网络的普通话智能评测关键技术研究

为改进传统普通话水平测试第四题测评中的不足,实现测评的智能化,减轻工作人员工作量,提升考试的公平性,采用基于深度神经网络的普通话智能评测关键技术,设计使用深度神经网络-隐马尔科夫模型的语音识别模型(DNN-HMM),识别考生表述的语音,更好地反映考生真实发音的标准程度;使用条件随机场训练分句模型,更加精准地判定考生发音的流畅程度;采用矢量空间模型,较好地评估考生词汇语法使用的规范程度。