Calcium Concentration Dependence of the Intergranular Film Thickness in Silicon Nitride

High-resolution electron microscopy and nano-beam analytical electron microscopy have been used to characterize both the intergranular silicate film thickness and its local composition in a series of high-purity Si₃N₄ ceramics doped with 0–450 at. ppm Ca. Calcium was detected at both two-grain junctions and triple junctions, even in the 80-ppm-Ca-doped specimen. The thickness of the intergranular film at two-grain junctions was found to depend sensitively on Ca content. In undoped material, the thickness was 1.0 ± 0.1 nm. With increasing Ca content, the thickness decreased in the dilute region (80 ppm Ca), but then increased. The variation in film thickness can be qualitatively understood in terms of the balance of three long-range forces acting normal to the film, namely the van der Waals dispersion force, a structural "steric" force, and an electrical-double-layer force. By comparing the measured thicknesses to those predicted, estimates for the structural correlation length and the inverse Debye length can be made. These estimates have values of ∼ 0.22 nm and approximately 0.3–0.5 nm, respectively, for the calcia-free and 80 ppm calcia materials.     

Ductal carcinoma in situ of the breast: correlation of pathologic and mammographic features with extent of disease

Optimal treatment of ductal carcinoma in situ (DCIS) of the breast requires an improved understanding of its pathologic extent and propensity for local recurrence. This study was performed to analyze mammographic and pathologic features of DCIS that might predict the extent of disease within the breast and facilitate treatment selection between lumpectomy alone, lumpectomy and radiotherapy, and mastectomy. At our institution, 60 cases of DCIS were diagnosed in 59 patients from June 1985 to February 1995 and form the basis of this retrospective study. Demographic and treatment-related information was obtained from hospital and tumor registry records. Mammograms were reviewed and size estimates of the abnormalities were determined. Pathologic slides from all cases were reviewed and classified according to size group, focality, nuclear grade, necrosis, and histologic subtype. DNA ploidy status and proliferation indices were available for 28 patients. Pathologically, 43 (72%) cases were < 15 mm, 14 (23%) were 16 to 40 mm, and 3 (5%) were > 40 mm. Five (8%) of the lesions were multicentric, 28 (47%) focal, and 27 (45%) multifocal. Thirty-three (55%) patients were treated by mastectomy, 16 (27%) by lumpectomy alone, and 11 (18%) by lumpectomy and radiation therapy. Mammographic size, histologic grade, presence or absence of necrosis, histologic subtype, DNA ploidy, and proliferative index were compared with pathologic size and focality by chi 2 analysis. Mammographic size correlated significantly with pathologic size (chi 2 = 11.3; P = 0.02) but underestimated the extent of disease in 9 cases. Although focality correlated significantly with pathologic size (chi 2 = 15.8; P = 0.003), the remaining histopathologic features did not significantly correlate with pathologic size or focality. Histopathologic features, including DNA studies, do not reliably predict the pathologic extent of DCIS, but mammographic size and focality do significantly correlate with pathologic size. Nevertheless, most cases of DCIS are small focal or multifocal lesions that are amenable to breast conservation approaches; further studies are needed to determine the appropriate use of lumpectomy, radiation therapy, and mastectomy in the treatment of DCIS.     

Ca2+-triggered peptide secretion in single cells imaged with green fluorescent protein and evanescent-wave microscopy

Green fluorescent protein fused to human chromogranin B or neuropeptide Y was expressed in PC12 cells and caused bright, punctate fluorescence. The fluorescent points colocalized with the endogenous secretory granule marker dopamine beta-hydroxylase. Stimulation of live PC12 cells with elevated [K+], or of permeabilized PC12 cells with Ca2+, led to Ca2+-dependent loss of fluorescence from neurites. Ca2+ stimulated secretion of both fusion proteins equally well. In living cells, single fluorescent granules were imaged by evanescent-wave fluorescence microscopy. Granules were seen to migrate; to stop, as if trapped by plasmalemmal docking sites; and then to disappear abruptly, as if through exocytosis. Evidently, GFP fused to secreted peptides is a fluorescent marker for dense-core secretory granules and may be used for time-resolved microscopy of single granules.     

An Enantioselective Total Synthesis of (+)-Elenoic Acid and the Non-Natural Enantiomers of Tetrahydroalstonine,Aricine, and Reserpinine

A simple enantioselective synthesis of (+)epi-elenoic acid is reported and its transformation into (+)-elenoic acid is described. Well-established procedures transform these compounds into the antipodes of tetrahydroalstonine, aricine, and reserpinine.     

Separation of stat-copoly(styrene/2-methoxyethyl methacrylate) samples according to composition by gradient high-performance liquid chromatography

Statistical copolymers of styrene (S) and 2-methoxyethyl methacrylate (MEMA) (13–87 mass % of the latter monomer) were investigated by gradient HPLC on columns with either silica or CN-bonded phase packings. The samples were injected in THF solution. The starting eluent was in each case a nonsolvent (isooctane or mixtures of isooctane with THF and methanol). Separation was achieved by increasing the concentration of THF or methanol in the eluent. A calibration mixture of four copolymers (26, 49, 62, and 87% MEMA) was investigated according to the principles of chromatographic cross-fractionation (CCF) by size exclusion chromatography and subsequent gradient HPLC. The influence of molar mass on HPLC retention was small and, for the samples investigated by CCF, independent of copolymer composition. The composition effect on detector signal was also studied. At 230 nm wavelength and with MEMA content in the range between 20 and 40%, the effect was small and caused the average composition calculated from CCF results to deviate from the directly measured value by not more than 0.2 or 0.8% for the two copolymers investigated.     

A Platform-Independent Methodology for Performance Estimation of Multimedia Signal Processing Applications

A methodological framework for performance estimation of multimedia signal processing applications on different implementation platforms is presented. The methodology derives a complexity profile which is characteristic for an application, but completely platform-independent. By correlating the complexity profile with platform-specific data, performance estimation results for different platforms are obtained. The methodology is based on a reference software implementation of the targeted application, but is, in constrast to instruction-level profiling-based approaches, fully independent of its optimization degree. The proposed methodology is demonstrated by example of an MPEG-4 Advanced Simple Profile (ASP) video decoder. Performance estimation results are presented for two different platforms, a specialized VLIW media processor and an embedded general-purpose RISC processor, showing a high accuracy of he methodology. The approach can be employed to assist in design decisions in the specification phase of new architectures, in the selection process of a suitable target platform for a multimedia application, or in the optimization stage of a software implementation on a specific platform.Hans-Joachim Stolberg received the Dipl.-Ing. degree in electrical engineering from the University of Hannover, Germany, in 1995.From 1995 to 1996, he worked at the NEC Information Technology Research Laboratories, Kawasaki, Japan, on efficient implementation of video compression algorithms. Since 1996, he has been with the Institute of Microelectronic Systems at the University of Hannover as a Research Assistant. During summer 2001, he was a Monbukagakusho Research Fellow at the Tokyo Institute of Technology, Japan. His current research interests include VLSI architectures for video signal processing, performance estimation of multimedia schemes, and profile-guided memory organization approaches for signal processing and multimedia applications.Mladen Bereković received the Dipl.-Ing. degree in electrical engineering from the University of Hannover, Germany, in 1995.Since then he has been a Research Assistant with the Institute of Microelectronic Systems of the University of Hannover. His current research interests include VLSI architectures for video signal processing, MPEG-4, System-on-Chip (SOC) designs, and simultaneously multi-threaded (SMT) processor architectures.Peter Pirsch received the Ing. grad. degree from the engineering college in Hannover, Germany, in 1966, and the Dipl.-Ing. and Dr.-Ing. degrees from the University of Hannover, in 1973 and 1979, respectively, all in electrical engineering.From 1966 to 1973 he was employed by Telefunken, Hannover, working in the Television Department. He became a Research Assistant at the Department of Electrical Engineering, University of Hannover, in 1973, a Senior Engineer in 1978. During 1979 to 1981 he was on leave, working in the Visual Communications Research Department, Bell Laboratories, Holmdel, NJ. During 1983 to 1986 he was Department Head for Digital Signal Processing at the SEL Research Center, Stuttgart, Germany. Since 1987 he is Professor in the Department of Electrical and Computer Engineering at the University of Hannover. He served as Vice President Research of the University of Hannover from 1998 to 2002.His present research includes architectures and VLSI implementations for image processing applications, rapid prototyping and design automation for DSP applications. He is the author or coauthor of more than 200 technical papers. He has edited a book on VLSI Implementations for Image Communications (Elsevier 1993) and is author of the book Architectures for Digital Signal Processing (John Wiley 1998).Dr. Pirsch is a member of the IEEE, the German Institute of Information Technology Engineers (ITG) and the German Association of Engineers (VDI). He was recipient of several awards: the NTG paper price award (1982), IEEE Fellow (1997), IEEE Circuits and Systems Golden Jubilee Medal (1999). He was member or chair of several technical program committees of international conferences and organizer of special sessions and preconference courses. He has held several administrative and technical positions with the IEEE Circuits and Systems Society and other professional organizations. Dr. Pirsch currently serves as Vice President Publications of the IEEE Circuits and Systems Society. Since 2000 he is chairman of the Accreditation Commission for Engineering and Informatics of the Accreditation Agency for Study Programs in Engineering, Informatics, Natural Science and Mathematics (ASIIN). Dr. Pirsch is chair of the VDI committee on Engineering Education.