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41.
Carla M.B. Gonçalves Liliana C. Tomé Helga Garcia Lúcia Brandão Adélio M. Mendes Isabel M. Marrucho 《Journal of food engineering》2013
Gas permeation properties (permeability, diffusivity and solubility coefficients) were determined for carbon dioxide and oxygen in poly(lactic acid) (PLA) films enriched with 0, 2, 4 and 10 wt.% of different antioxidants, at three temperatures, 284, 293 and 303 K, using a time-lag apparatus. Three antioxidants, a natural, α-tocopherol (AT), and two synthetic, butylated hydroxytoluene (BHT) and tert-butylhydroquinone (TBHQ), were tested. DSC results show that the polymer glass transition temperature slightly decreases with the increase of the antioxidant content. The crystallinity degree of PLA films decreases with the addition of BHT and TBHQ, whereas the incorporation of AT increases the crystallinity of PLA films. The permeability towards water vapour, at 299 K and 45% of relative humidity, and surface energy show a decreased in the wettability of the prepared materials with the increase of the antioxidants content. The incapacity to measure gas permeation in PLA films with 10 wt.% of AT and BHT incorporated was due to phase separation, proved by SEM images. The CO2 and O2 permeation results show that PLA barrier properties can be improved by the incorporation of antioxidants but are strongly dependent on the amount and structure of the antioxidant added. 相似文献
42.
Sperka T Boross P Eizert H Tözsér J Bagossi P 《Protein engineering, design & selection : PEDS》2006,19(8):369-375
To explore the role of residues being close to the catalytic aspartates in the higher pH optimum and in the lower dimer stability of human foamy virus (HFV) protease (PR) in comparison with human immunodeficiency virus type 1 (HIV-1) protease, single (Q8R, H22L, S25T, T28D) and double (Q8R-T28D, H22L-T28D) mutants were created based on sequence alignments and on the molecular model of HFV PR. The wild-type and mutant enzymes were expressed in fusion with maltose binding protein in Escherichia coli and the fusion proteins were purified by affinity chromatography. Specificity constant of most mutants was lower, but the value of Q8R-T28D double mutant enzyme was higher than that of the wild-type HFV PR. Furthermore, urea denaturation at two pH values and pH optimum values showed an increased stability and pH optimum for most mutants. These results suggest that the mutated residues may not be responsible for the higher pH optimum of HFV PR, but they may contribute to the lower dimer stability as compared with that of HIV-1 PR. 相似文献
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44.
Christina Kopp Afshin Hosseini Shiva P. Singh Petra Regenhard Hamed Khalilvandi-Behroozyar Helga Sauerwein Manfred Mielenz 《International journal of molecular sciences》2014,15(11):21401-21418
The transition period in dairy cows (3 weeks prepartum until 3 weeks postpartum) is associated with substantial mobilization of energy stores, which is often associated with metabolic diseases. Nicotinic acid (NA) is an antilipolytic and lipid-lowering compound used to treat dyslipidaemia in humans, and it also reduces non-esterified fatty acids in cattle. In mice the G-protein coupled receptor 109A (GPR109A) ligand NA positively affects the secretion of adiponectin, an important modulator of glucose and fat metabolism. In cattle, the corresponding data linking NA to adiponectin are missing. Our objective was to examine the effects of NA on adiponectin and AMPK protein abundance and the expression of mRNAs of related genes such as chemerin, an adipokine that enhances adiponectin secretion in vitro. Differentiated bovine adipocytes were incubated with pertussis toxin (PTX) to verify the involvement of GPR signaling, and treated with 10 or 15 µM NA for 12 or 24 h. NA increased adiponectin concentrations (p ≤ 0.001) and the mRNA abundances of GPR109A (p ≤ 0.05) and chemerin (p ≤ 0.01). Pre-incubation with PTX reduced the adiponectin response to NA (p ≤ 0.001). The NA-stimulated secretion of adiponectin and the mRNA expression of chemerin in the bovine adipocytes were suggestive of GPR signaling-dependent improved insulin sensitivity and/or adipocyte metabolism in dairy cows. 相似文献
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Cappiello A Famiglini G Palma P Pierini E Termopoli V Trufelli H 《Mass spectrometry reviews》2011,30(6):1242-1255
This review article will give an up-to-date and exhaustive overview on the efficient use of electron ionization (EI) to couple liquid chromatography and mass spectrometry (LC-MS) with an innovative interface called Direct-EI. EI is based on the gas-phase ionization of the analytes, and it is suitable for many applications in a wide range of LC-amenable compounds. In addition, thanks to its operating principles, it prevents unwelcome matrix effects (ME). In fact, although atmospheric pressure ionization (API) methodologies have boosted the use of LC-MS, the related analytical methods are sometime affected by inaccurate quantitative results, due to unavoidable and unpredictable ME. In addition, API's soft ionization spectra always demand for costly and complex tandem mass spectrometry (MS/MS) instruments, which are essential to acquire an "information-rich" spectrum and to obtain accurate quantitative information. In EI a one-stage analyzer is sufficient for a qualitative investigation and MS/MS detection is only used to improve sensitivity and to cut chemical noise. The technology illustrated here provides a robust and straightforward access to classical, well-characterized EI data for a variety of LC applications, and readily interpretable spectra for a wide range of areas of research. The Direct-EI interface can represent the basis for a forthcoming universal LC-MS detector for small molecules. 相似文献
47.
Frouke Vermeulen Helga D'Havé Nico W. Van den Brink Lieven Bervoets 《The Science of the total environment》2009,407(5):1775-1783
Hair has been proven to be suitable for non-destructive and non-invasive exposure assessments in human and mammal populations. A previous study with European hedgehog (Erinaceus europaeus) showed that, for some metals, hair and spine metal concentrations were positively correlated to levels in liver, kidney and muscle. Although blood has been studied in a wide variety of species, the relationship between hair and blood metal concentrations has yet to be quantified in many mammalian species. Tissue concentrations from hedgehogs residing in a park with known metal pollution were compared with those from a reference park and correlations between contaminant levels in hair and blood, and spines and blood were studied. Moreover, the relative distribution of arsenic and metals in hair, spines and blood was determined. Elevated concentrations were found in hedgehogs residing in the polluted site for As (8.2 μg/g, 6.3 μg/g, 3.6 μg/ml), Cd (0.48 μg/g, 0.17 μg/g, 0.02 μg/ml) and Pb (7.6 μg/g, 7.3 μg/g, 54 μg/ml), in hair, spines and blood respectively. Positive correlations were identified for exposure levels between hair and blood as well as between spines and blood for three elements (As, Cd, and Pb), whereas a negative correlation was found between Cr concentrations in spines and blood. In conclusion, hair and spines can be used to monitor blood concentrations of some metals, although more data are needed on uptake from the food chain and on the incorporation dynamics of these contaminants. 相似文献
48.
A sensitive and selective method for determination of the uracil content in human DNA was first developed on the basis of high-performance liquid chromatography-tandem mass spectrometry. Uracil was excised from DNA using uracil DNA glycosylase. The released uracil was derivatized with 4-bromomethyl-7-methoxycoumarin, thereby forming bis-N,N'-(4-methylene-7-methoxycoumaryl)-uracil (uracil-MMC). 15N2-Uracil was used as an internal standard. The analytes were separated on an Adsorbsphere XL ODS column. A SCIEX API III tandem mass spectrometer equipped with a turbo ion-spray interface was used as the detector. Multiple reaction monitoring using the parent --> product ion combinations of m/z 489 --> 232 and 491 --> 233 were used to detect uracil-MMC and the internal standard, respectively. The detection limit for this assay is <1.0 x 10(-10) mol/L uracil, and the linearity is from 1.0 x 10(-10) to 2.5 x 10(-6) mol/L. The method was used for determination of uracil in human DNA. Our data show that the uracil levels in human DNA isolated from peripheral white blood cells did not differ between subjects with folate deficiency and subjects with normal red cell folate levels. 相似文献
49.
Lejeune Helga; Ferrara André; Simons Frédéric; Wearden John H. 《Canadian Metallurgical Quarterly》1997,23(2):211
Thirty rats received training on a peak-interval procedure, where a baseline with an 20-s time of reinforcement was interspersed among cyclic transition to other reinforcement time values (10, 20, 30, or 40 s), each of which was either in force for only a single session or for 3 sessions. Peak times were close to the time of reinforcement on the 20-s baseline and tracked the new reinforcement times both closely (but not exactly) and very rapidly. Peak time during transitions was affected by the criterion value in force on the previous session, exhibiting a proactive interference effect. Analysis of individual peak times during a session showed that transitions from lower to higher reinforcement time values were usually characterized by abrupt jumps in peak time, whereas descending transitions were mostly smooth but rapid. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
50.