Thermally induced structural and morphological changes of CdSe/CdS octapods

Branched nanostructures are of great interest because of their promising optical and electronic properties. For successful and reliable integration in applications such as photovoltaic devices, the thermal stability of the nanostructures is of major importance. Here the different domains (CdSe cores, CdS pods) of the heterogeneous octapods are shown to have different thermal stabilities, and heating is shown to induce specific shape changes. The octapods are heated from room temperature to 700 °C, and investigated using (analytical and tomographic) transmission electron microscopy (TEM). At low annealing temperatures, pure Cd segregates in droplets at the outside of the octapods, indicating non-stochiometric composition of the octapods. Furthermore, the tips of the pods lose their faceting and become rounded. Further heating to temperatures just below the sublimation temperature induces growth of the zinc blende core at the expense of the wurtzite pods. At higher temperatures, (500-700 °C), sublimation of the octapods is observed in real time in the TEM. Three-dimensional tomographic reconstructions reveal that the four pods pointing into the vacuum have a lower thermal stability than the four pods that are in contact with the support.     

Centrifugal Casting of Tubular Perovskite Membranes

Dense tubular membranes were produced by centrifugal casting of an aqueous suspension, containing powder particles of the mixed-conducting perovskite La_0.5Sr_0.5CoO_3−δ and a dispersant. The resulting green bodies were dried and sintered to produce tubes with a maximum length of 12 cm, having a relative density higher than 92%. The particle morphology, the amount of dispersant and its burnout appeared to influence the quality of the final product. Oxygen permeation measurements were conducted in the temperature range 850°–950°C in Air/He gradients. Results were found to be consistent with data reported for disk-type membranes.     

Possibilities and impossibilities of magnetic nanoparticle use in the control of infectious biofilms

Targeting of chemotherapeutics towards a tumor site by magnetic nanocarriers is considered promising in tumor-control. Magnetic nanoparticles are also considered for use in infection-control as a new means to prevent antimicrobial resistance from becoming the number one cause of death by the year 2050. To this end, magnetic nanoparticles can either be loaded with an antimicrobial for use as a delivery vehicle or modified to acquire intrinsic antimicrobial properties. Magnetic nanoparticles can also be used for the local generation of heat to kill infectious microorganisms. Although appealing for tumor-and infectioncontrol, injection in the blood circulation may yield reticuloendothelial uptake and physical obstruction in organs that yield reduced targeting efficiency. This can be prevented with suitable surface modification. However, precise techniques to direct magnetic nanoparticles towards a target site are lacking. The problem of precise targeting is aggravated in infection-control due to the micrometer-size of infectious biofilms, as opposed to targeting of nanoparticles towards centimeter-sized tumors. This review aims to identify possibilities and impossibilities of magnetic targeting of nanoparticles for infection-control. We first review targeting techniques and the spatial resolution they can achieve as well as surface-chemical modifications of magnetic nanoparticles to enhance their targeting efficiency and antimicrobial efficacy.It is concluded that targeting problems encountered in tumor-control using magnetic nanoparticles, are neglected in most studies on their potential application in infection-control. Currently biofilm targeting by smart, self-adaptive and pH-responsive, antimicrobial nanocarriers for instance, seems easier to achieve than magnetic targeting. This leads to the conclusion that magnetic targeting of nanoparticles for the control of micrometer-sized infectious biofilms may be less promising than initially expected.However, using propulsion rather than precise targeting of magnetic nanoparticles in a magnetic field to traverse through infectious-biofilms can create artificial channels for enhanced antibiotic transport.This is identified as a more feasible, innovative application of magnetic nanoparticles in infection-control than precise targeting and distribution of magnetic nanoparticles over the depth of a biofilm.     

In Situ Topotactic Transformation of an Interstitial Alloy for CO Electroreduction

Electrochemical reduction of CO to value-added products holds promise for storage of energy from renewable sources. Copper can convert CO into multi-carbon (C₂₊) products during CO electroreduction. However, developing a Cu electrocatalyst with a high selectivity for CO reduction and desirable production rates for C₂₊ products remains challenging. Herein, highly lattice-disordered Cu₃N with abundant twin structures as a precursor electrocatalyst is examined for CO reduction. Through in situ activation during the CO reduction reaction (CORR) and concomitant release of nitrogen, the obtained metallic Cu° catalyst particles inherit the lattice dislocations present in the parent Cu₃N lattice. The de-nitrified catalyst delivers an unprecedented C₂₊ Faradaic efficiency of over 90% at a current density of 727 mA cm⁻² in a flow cell system. Using a membrane electrode assembly (MEA) electrolyzer with a solid-state electrolyte (SSE), a 17.4 vol% ethylene stream and liquid streams with concentration of 1.45 m and 230 × 10⁻³ m C₂₊ products at the outlet of the cathode and SSE-containment layer are obtained.     

Collecting Statistics Over Runtime Executions

We present an extension to linear-time temporal logic (LTL) that combines the temporal specification with the collection of statistical data. By collecting statistics over runtime executions of a program we can answer complex queries, such as “what is the average number of packet transmissions' in a communication protocol, or “how often does a particular process enter the critical section while another process remains waiting' in a mutual exclusion algorithm. To decouple the evaluation strategy of the queries from the definition of the temporal operators, we introduce algebraic alternating automata as an automata-based intermediate representation. Algebraic alternating automata are an extension of alternating automata that produce a value instead of acceptance or rejection for each trace. Based on the translation of the formulas from the query language to algebraic alternating automata, we obtain a simple and efficient query evaluation algorithm. The approach is illustrated with examples and experimental results.     

Checking Finite Traces Using Alternating Automata

Alternating automata have been commonly used as a basis for static verification of reactive systems. In this paper we show how alternating automata can be used in runtime verification. We present three algorithms to check at runtime whether a reactive program satisfies a temporal specification, expressed by a linear-time temporal logic formula. The three methods start from the same alternating automaton but traverse the automaton in different ways: depth-first, breadth-first, and backwards, respectively. We then show how an extension of these algorithms, that collects statistical data while verifying the execution trace, can be used for a more detailed analysis of the runtime behavior. All three methods have been implemented and experimental results are presented.     

Therapeutic Prospects of Exon Skipping for Epidermolysis Bullosa

Epidermolysis bullosa is a group of genetic skin conditions characterized by abnormal skin (and mucosal) fragility caused by pathogenic variants in various genes. The disease severity ranges from early childhood mortality in the most severe types to occasional acral blistering in the mildest types. The subtype and severity of EB is linked to the gene involved and the specific variants in that gene, which also determine its mode of inheritance. Current treatment is mainly focused on symptomatic relief such as wound care and blister prevention, because truly curative treatment options are still at the preclinical stage. Given the current level of understanding, the broad spectrum of genes and variants underlying EB makes it impossible to develop a single treatment strategy for all patients. It is likely that many different variant-specific treatment strategies will be needed to ultimately treat all patients. Antisense-oligonucleotide (ASO)-mediated exon skipping aims to counteract pathogenic sequence variants by restoring the open reading frame through the removal of the mutant exon from the pre-messenger RNA. This should lead to the restored production of the protein absent in the affected skin and, consequently, improvement of the phenotype. Several preclinical studies have demonstrated that exon skipping can restore protein production in vitro, in skin equivalents, and in skin grafts derived from EB-patient skin cells, indicating that ASO-mediated exon skipping could be a viable strategy as a topical or systemic treatment. The potential value of exon skipping for EB is supported by a study showing reduced phenotypic severity in patients who carry variants that result in natural exon skipping. In this article, we review the substantial progress made on exon skipping for EB in the past 15 years and highlight the opportunities and current challenges of this RNA-based therapy approach. In addition, we present a prioritization strategy for the development of exon skipping based on genomic information of all EB-involved genes.     

Fast DNA translocation through a solid-state nanopore

We report experiments and modeling of translocation of double-strand DNA through a siliconoxide nanopore. Long DNA molecules with different lengths ranging from 6500 to 97000 base pairs have been electrophoretically driven through a 10 nm pore. We observe a power-law caling of the translocation time with the length, with an exponent of 1.27. This nonlinear scaling is strikingly different from the well-studied linear behavior observed in similar experiments performed on protein pores. We present a theoretical model where hydrodynamic drag on the ection of the polymer outside the pore is the dominant force counteracting the electrical driving force. We show that this applies to our experiments, and we derive a power-law scaling with an exponent of 1.22, in good agreement with the data.     

Non-Darcian Airflow through Ceramic Foams

This paper presents the first results on fluid flow through (laboratory-made) ceramic foams. Pressure drop (ΔP) vs airflow rate ( U ) measurements show that Darcy's law does not hold and that the non-Darcian airflow through the foams satisfies an equation of the form ΔP = aU + bU ². A permeability quotient is defined, which appears to be a qualitative measure for the average pore size of a foam. A Reynolds number defined in terms of this quotient can be used to estimate the Darcian flow range for a certain fluid.     

水冷型和风冷型日晒色牢度试验机

日晒色牢度试验机自诞生以来，经历了转鼓式风冷、转鼓式水冷和平板式风冷三个时期。该文重点分析了风冷和水冷机型的优缺点，并结合日晒色牢度测试的常用标准和实际应用情况，就风冷和水冷机型对检测结果的影响和不同机型之间检测结果的可比性问题，进行了讨论。