Regulation of V(D)J recombination activator protein RAG-2 by phosphorylation

Antigen receptor genes are assembled by site-specific DNA rearrangement. The recombination activator genes RAG-1 and RAG-2 are essential for this process, termed V(D)J rearrangement. The activity and stability of the RAG-2 protein have now been shown to be regulated by phosphorylation. In fibroblasts RAG-2 was phosphorylated predominantly at two serine residues, one of which affected RAG-2 activity in vivo. The threonine at residue 490 was phosphorylated by p34cdc2 kinase in vitro; phosphorylation at this site in vivo was associated with rapid degradation of RAG-2. Instability was transferred to chimeric proteins by a 90-residue portion of RAG-2. Mutation of the p34cdc2 phosphorylation site of the tumor suppressor protein p53 conferred a similar phenotype, suggesting that this association between phosphorylation and degradation is a general mechanism.     

Novel technique for SiO2 formed by liquid-phasedeposition for low-temperature processed polysilicon TFT

A technique for SiO₂ formation by liquid-phase deposition (LPD) at nearly room temperature for low-temperature processed (LTP) polysilicon thin-film transistor (poly-Si TFT) was developed. LPD SiO₂ film with a lower P-etch rate shows a dense structure. LPD SiO₂ also exhibits good electrical characteristics. LTP poly-Si thin-film transistors (TFTs) with LPD SiO ₂ as the gate insulator have been fabricated and investigated. Their characteristics indicate performance adequate for their use as pixel transistors in liquid crystal displays (LCDs)     

Tyrosine-dependent basolateral sorting signals are distinct from tyrosine-dependent internalization signals

Converting cysteine 543 to tyrosine in the influenza virus hemagglutinin (HA) introduces both a basolateral sorting signal and an internalization signal into the HA cytoplasmic domain. Another HA mutant, HA+8, contains eight additional amino acids at the end of the cytoplasmic domain that include a powerful internalization signal. HA+8 was also sorted efficiently to the basolateral surface of Madin-Darby canine kidney cells. The simplest explanation for the observation that multiple sorting phenotypes depend upon the same small amino acid sequence is that certain tyrosine-based internalization signals might also function as basolateral sorting signals. To test this hypothesis, second-site mutations were introduced into HA C543Y or HA+8 to determine if the internalization and basolateral sorting functions can be separated. For HA C543Y, the same sequence positions were important for both basolateral sorting and internalization, but the two functions responded differently to individual amino acid replacements, indicating that they were distinct. For HA+8, the basolateral sorting signal required the same tyrosine as the internalization signal, but did not share any other characteristics. Thus, even when basolateral sorting signals that depend on tyrosine overlap or are co-linear with internalizations signals, the two sorting processes are sensitive to different characteristics of the sequence.     

The sub-rating channel assignment strategy for PCS hand-offs

A new personal communications services (PCS) hand-off scheme is proposed. This scheme provides for hand-off to radio ports on which there is no free channel by “sub-rating” an existing connection. With sub-rating, an occupied full-rate channel is temporarily divided into two half-rate channels: one to serve the existing call and the other to serve the hand-off request. The blocking probabilities (combined forced terminations of existing calls and blocking of new call attempts) of this new scheme compare favorably with the standard scheme (nonprioritizing) and the previously proposed prioritizing schemes. The costs for this scheme are presented and discussed, as well as the additional procedural complexity of implementing on-the-fly sub-rating and the impact of continuing the conversation on a lower rate channel (which may lower speech quality of increase battery drain). Analytical models and simulations investigating the traffic impacts are presented, as are the results that show that even in the highest offered load considered a 3-min conversation in the busy hour experiences less than half a second of sub-rated conversation on average and only about 3% of the calls experience more than 5.12 s of sub-rated conversation. This scheme can increase capacity by 8-35% for systems with 1% call incompletion probability     

用红外热象技术研究有凸台热源倾斜板的散热

本文介绍红外热象技术在工程热物理研究中某些应用的探索。作为实例，通过热象技术，实验研究了具有水平凸台离散热源的倾斜板的散热，在研究它的自然对流换热部份结合利用激光全息干涉技术。此间着重于分析倾斜角对散热的影响。     

Principles for radiation of time-harmonic elementary current in magnetized electron plasma half-space

The radiation emitted by a time-harmonic elementary current in magnetized electron plasma half-space, bounded by an ideal electric plane, is investigated using an exact formulation. It is found that: conventional image principle holds for vertical magnetization case; while for parallel magnetization case, coupled image principle seems to be valid.     

Pentacene-based organic thin-film transistors

Organic thin-film transistors using the fused-ring polycyclic aromatic hydrocarbon pentacene as the active electronic material have shown mobility as large as 0.7 cm²/V-s and on/off current ratio larger than 10⁸; both values are comparable to hydrogenated amorphous silicon devices. On the other hand, these and most other organic TFT's have an undesirably large subthreshold slope. We show here that the large subthreshold slope typically observed is not an intrinsic property of the organic semiconducting material and that devices with subthreshold slope similar to amorphous silicon devices are possible     

An approach to the identifiable parameters of a manipulator

This article deals with the minimal parameters of a manipulator in the least squares sense, so that the minimal parameters are equivalent to the identifiable parameters. The least squares concept is used to introduce terminology for the minimal linear combinations (MLCs) of the system parameters that define a set of linear combinations of the system parameters. The number of elements of the set is minimal, yet the set still completely determines the system. Furthermore, it is shown that the problem of finding a set of MLCs of a manipulator can be simplified to that of finding two individual sets of MLCs that determine the entries of the inertia matrix and the gravity load. Although the approach is applied to the inertia constants of composite bodies to obtain a set of MLCs identical to an earlier one, the result is newly interpreted in the least squares sense. The approach itself is a new method for finding the identifiable parameters of a manipulator, and it yields some new insight into the manipulator dynamics. The crucial feature is that a set of MLCs found by using the present approach is guaranteed to be identifiable. The earlier approaches always require an identification method to verify the results. An equivalence theorem is also presented that rigorously states the equivalence between the different sets of minimal parameters. © 1994 John Wiley & Sons, Inc.     

潘庄引黄灌区泥沙处理对策探讨

潘庄引黄灌区在多年的引黄泥沙处理利用工作中,探索并总结出一套适合灌区特点的泥沙处理模式,取得了较好的效果。主要针对灌区引黄灌溉中的泥沙处理问题,在综述了灌区泥沙处理和利用的现状和新形势下传统泥沙处理方式所存在问题的基础上,从自然条件、工程条件等方面进行泥沙处理的探讨。