The Production of Low-Alcohol Wines by Aerobic Yeasts

Aerobic yeasts of the genera Pichia and Williopis are commonly regarded as spoilage yeasts of beer and wine by causing turbidity, a surface film of yeast growth and often an excessive estery flavour. However, their ability to utilise sugars oxidatively for cell growth with the production of estery and other flavours of wine with only minimal production of ethanol suggests a method for the production of low-alcohol wines of pleasant “fermented” flavour without the need for additional equipment to remove alcohol by dialysis, reverse osmosis or distillation, or without the excessive sweetness remaining from arrested fermentation. Three strains of Pichia and one of Williopsis were examined for their ability to produce approximately 3%(v/v) ethanol and a good estery and fruity flavour. With normal anaerobic fermentation conditions, or with gentle stirring to prevent formation of a surface film, excessive amounts of alcohol were produced from grape juice of 15% or 20% (w/v) initial sugar concentration. However, an acceptably flavoured wine of alcohol content < 3% was produced by agitation and aeration during fermentation. The ethanol formed in the early stages of culture was oxidised to a final level < 3%, with the production of cell mass and an acceptable flavour.     

Failure Analysis of a Location Axle in Tracked Tractor

In this study, a failure analysis was performed on the location axle (brace axle) of a blade on a tracked tractor used in agricultural areas. The analysis included evaluation of the macrostructure of the damage and the microstructure of the material, as well as a chemical analysis of the material. A commercial finite element method software package was used to determine the behavior of the location axle under the defined boundary conditions. After all the examination steps were completed, the main reason for the damage was determined to be manufacture and material error despite the presence of fatigue tracks in the damaged area. In addition, a heat treatment was carried out with the specimens taken from the damaged area and microstructure was re-examined. The experimental and simulation evaluation results provide a technical basis for suggestions to prevent future damage to the location axle.     

The Manavgat Project of Turkey: Water,an Economic Good

In spite of the fact that abundance of water creates problems and sometimes disasters, much of the problem with water lies with its scarcity. Water shortages in a region occur mainly for two reasons. The most important one is associated with climatic conditions, whereas the other has to do with the fact that the location of the water resource does not coincide with where it is needed. This is where engineering and technology come into play along with a handsome amount of capital requirement. As is the case for almost all engineering projects, project economy entails both technical and economic feasibility, which in turn requires that water should be treated as an economic commodity.     

Lateral Weir Flow Model using a Curve Fitting Analysis

A numerical approach is considered for flow over side weirs as a substantial part of distribution channels in irrigation systems and treatment units. The model is based on the energy principle and a curve-fitting technique. For this purpose, the side weir was divided into elementary strips to develop generalized equations for discharge and surface profile. The change in water surface elevation towards the weir crest and the inclination of the deflected flow over the weir were also taken into account. Dimensionless parameters were used and the normalized equations solved to obtain the hydraulic parameters of side weirs. The results were plotted to determine general relationships based on the curve-fitting technique. A practical application of the derived equations to obtain hydraulic parameters of side weirs is performed using literature data.     

Dipole moments of styrene/4-vinylpyridine copolymers—II: Relation to microstructure

Mean-square dipole moment values of styrene/4-vinylpyridine (S4VP) copolymers determined at 25–60°C in 1,4-dioxane solutions were related to the microstructure of the chains. Data on the synthesis and dipole moments of S4VP copolymers were used to calculate the distribution of triad fractions and effective dipole moments of styrene, S-centred, and 4-vinylpyridine, V-centred, fractions in the copolymer chains. The relative magnitudes of the effective dipole moment values calculated for each type of triad reflect the effect of dipole–dipole interactions on these values.     

Antioxidant and anticholinesterase evaluation of selected Turkish Salvia species

Since Salvia species (Lamiaceae) have been recorded to be used against memory loss in European folk medicine, we herein examined in vitro anticholinesterase and antioxidant activities of 56 extracts prepared with petroleum ether, chloroform, ethyl acetate and methanol obtained from 14 Salvia species (Salvia albimaculata Hedge and Hub, Salvia aucheri Bentham var. canescens Boiss and Heldr, Salvia candidissima Vahl. ssp. occidentalis, Salvia ceratophylla L., Salvia cryptantha Montbret and Bentham, Salvia cyanescens Boiss and Bal., Salvia frigida Boiss, Salvia forskahlei L., Salvia halophilaHedge, Salvia migrostegia Boiss and Bal., Salvia multicaulis Vahl., Salvia sclarea L., Salvia syriaca L., Salvia verticillata L. ssp. amasiaca) growing in Turkey. The antioxidant activities were assessed by both chemical and enzymatic methods against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging and xanthine/xanthine oxidase (XO) system generated superoxide anion radical inhibition. Anticholinesterase effect of the extracts was tested against both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) at concentrations of 0.2 and 1 mg/ml using a microplate-reader assay based on the Ellman method. Most of the extracts did not show any activity against AChE at 0.2 mg/ml, while the chloroform extracts had noticeable inhibition against BChE between 47.7% and 74.7%. The most active extracts at 1 mg/ml for AChE inhibition were observed to be petroleum ether extract of Salvia albimaculata (89.4%) and chloroform extract of Salvia cyanescens (80.2%), whereas ethyl acetate extracts of Salvia frigida and Salvia migrostegia, chloroform extracts of Salvia candidissima ssp. occidentalis and Salvia ceratophylla, as well as petroleum ether extract of Salvia cyanescens were found to inhibit potently BChE (92.2%, 89.6%, 91.1%, 91.3%, and 91.8%, respectively). Particularly, the ethyl acetate and methanol extracts were observed to be highly active against both DPPH and XO. Our data indicates that nonpolar extracts of Salvia species for anticholinesterase activity and the polar extracts for antioxidant activity are worth further phytochemical evaluation for identifying their active components.     

Shape memory behavior of FeNiCoTi single and polycrystals

We present experimental and theoretical evidence of thermoelastic martensites in Fe29Ni18Co4Ti alloys. In this class of alloys, the high strength in the austenite domains limits the slip deformation as verified with transmission electron microscopy. The restriction of slip permits a higher degree of recoverability of the transformation. Using both single crystals with [123] orientation and polycrystals, the appearance of martensite plates upon deformation, and their reversion back to austenite upon heating (the shape memory effect), is revealed with in-situ optical microscopy. Theoretical results for the transformation strains and the detwinning of martensite are presented, which demonstrate convincingly the potential of these classes of alloys. Electrical resistance measurements identified the stress and temperature levels at the onset of forward and reverse transformations in isothermal deformation and thermal cycling experiments, respectively. The return of the electrical resistance to its reference value, upon austenite to martensite followed by martensite to austenite transformation, verified the recovery in the transformation strains measured in the experiments.     

In silico discovery of significant pathways in colorectal cancer metastasis using a two‐stage optimisation approach

Accurate and reliable modelling of protein–protein interaction networks for complex diseases such as colorectal cancer can help better understand mechanism of diseases and potentially discover new drugs. Different machine learning methods such as empirical mode decomposition combined with least square support vector machine, and discrete Fourier transform have been widely utilised as a classifier and for automatic discovery of biomarkers for the diagnosis of the disease. The existing methods are, however, less efficient as they tend to ignore interaction with the classifier. In this study, the authors propose a two‐stage optimisation approach to effectively select biomarkers and discover interactions among them. At the first stage, particle swarm optimisation (PSO) and differential evolution (DE) are used to optimise parameters of support vector machine recursive feature elimination algorithm, and dynamic Bayesian network is then used to predict temporal relationship between biomarkers across two time points. Results show that 18 and 25 biomarkers selected by PSO and DE‐based approach, respectively, yields the same accuracy of 97.3% and F1‐score of 97.7 and 97.6%, respectively. The stratified analysis reveals that Alpha‐2‐HS‐glycoprotein was a dominant hub gene with multiple interactions to other genes including Fibrinogen alpha chain, which is also a potential biomarker for colorectal cancer.Inspec keywords: cancer, proteins, particle swarm optimisation, evolutionary computation, support vector machines, recursive functions, Bayes methods, genetics, molecular biophysics, medical computingOther keywords: colorectal cancer metastasis, two‐stage optimisation approach, protein–protein interaction networks, biomarkers, particle swarm optimisation, differential evolution, support vector machine recursive feature elimination, dynamic Bayesian network, stratified analysis, Alpha‐2‐HS‐glycoprotein, hub gene, Fibrinogen alpha chain     

All‐Carbon Nanoarchitectures as High‐Performance Separation Membranes with Superior Stability

The application of graphene‐based membranes is hindered by their poor stability under practical hydrodynamic conditions. Here, nanocarbon architectures are designed by intercalating surface‐functionalized, small‐diameter, multi‐walled carbon nanotubes (MWCNTs) into reduced graphene oxide (rGO) sheets to create highly stable membranes with improved water permeability and enhanced membrane selectivity. With the intercalation of 10 nm diameter MWCNTs, the water permeability reaches 52.7 L m^?2 h^?1 bar^?1, which is 4.8 times that of pristine rGO membrane and five to ten times higher than most commercial nanofiltration membranes. The membrane also attains almost 100% rejection for three organic dyes of different charges. More importantly, the membrane can endure a turbulent hydrodynamic flow with cross‐flow rates up to 2000 mL min^?1 and a Reynolds number of 4667. Physicochemical characterization reveals that the inner graphitic walls of the MWCNTs can serve as spacers, while nanoscale rGO foliates on the outer walls interconnect with the assimilated rGO sheets to instill superior membrane stability. In contrast, intercalating with single‐walled nanotubes fails to reproduce such stability. Overall, this nanoarchitectured design is highly versatile in creating both graphene‐rich and CNT‐rich all‐carbon membranes with engineered nanochannels, and is regarded as a general approach in obtaining stable membranes for realizing practical applications of graphene‐based membranes.     

Effect of harvesting hour on some physical and mechanical properties of Rosa damascena Mill

BACKGROUND: Mechanization of Rosa damascena flower harvest is limited because of the asynchronous flowering period, the necessity for the harvest operation to take place in the early hour of the morning, and a lack of knowledge regarding the physical–mechanical properties of R. damascena. This study investigated the effect of harvesting hour on some physical–mechanical properties of R. damascena. RESULTS: The terminal velocity of the rose flower ranged from 4.00 to 2.38 m s^?1. The highest picking force was found to be 6.29 N for the harvesting hour of 06:00 am. Tear forces of petal changed from 1.54 to 0.97 N for the harvesting hour of 06:00 am and 12:00 pm, respectively. The tension force of pedicel varied between 8.25 and 7.76 N. The weight of the rose flower was measured as an average of 1.61 and 1.50 g at 06:00 am and 12:00 pm, respectively. Horizontal and vertical projection areas of rose flowers were positively correlated with harvesting hour, with an R² of 0.97 and 0.75, respectively. Results showed that there was a minor loss in colour for both inner and outer surfaces of petals. CONCLUSION: The results of physical–mechanical properties of R. damascena obtained from this study should be considered for the operation of flower harvest mechanization. Copyright © 2011 Society of Chemical Industry