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61.
Giannakis E Samuel CS Hewitson TD Boon WM Macris M Reeve S Lawrence J Ian Smith A Tregear GW Wade JD 《Proteomics. Clinical applications》2009,3(10):1211-1224
Kidney failure is a major health problem worldwide. Patients with end-stage renal disease require intensive medical support by dialysis or kidney transplantation. Current methods for diagnosis of kidney disease are either invasive or insensitive, and renal function may decline by as much as 50% before it can be detected using current techniques. The goal of this study was, therefore, to identify biomarkers of kidney disease (associated with renal fibrosis) that can be used for the development of a non-invasive clinical test for early disease detection. We utilized two protein-profiling technologies (SELDI-TOF MS and 2-D) to screen the plasma and kidney proteome for aberrantly expressed proteins in an experimental mouse model of unilateral uretric obstruction, which mimics the pathology of human renal disease. Several differentially regulated proteins were detected at the plasma level of day-3-obstructed animals, which included serum amyloid A1, fibrinogen α, haptoglobin precursor protein, haptoglobin and major urinary proteins 11 and 8. Differentially expressed proteins detected at the tissue level included ras-like activator protein 2, haptoglobin precursor protein, malate dehydrogenase, α enolase and murine urinary protein (all p<0.05 versus controls). Immunohistochemistry was used to confirm the up-regulation of fibrinogen. Interestingly, these proteins are largely separated into four major classes: (i) acute-phase reactants (ii) cell-signaling molecules (iii) molecules involved in cell growth and metabolism and (iv) urinary proteins. These results provide new insights into the pathology of obstructive nephropathy and may facilitate the development of specific assay(s) to detect and monitor renal fibrosis. 相似文献
62.
Ian T. Baldwin Lynn Staszak-Kozinski Robert Davidson 《Journal of chemical ecology》1994,20(9):2345-2371
Some postfire annuals with dormant seeds use heat or chemical cues from charred wood to synchronize their germination with the postfire environment. We report that wood smoke and polar extracts of wood smoke, but not the ash of burned wood, contain potent cue(s) that stimulate germination in the postfire annual plant,Nicotiana attenuata. We examined the responses of seeds from six populations of plants from southwest Utah to extracts of smoke and found the proportion of viable seeds that germinated in the presence of smoke cues to vary between populations but to be consistent between generations. With the most dormant genotypes, we examine three mechanisms by which smoke-derived chemical cues may stimulate germination (chemical scarification of the seed coat and nutritive- and signal-mediated stimulation of germination) and report that the response is consistent with the signal-mediated mechanism. The germination cue(s) found in smoke are produced by the burning of hay, hardwood branches, leaves, and, to a lesser degree, cellulose. Moreover, the cues are found in the common food condiment, liquid smoke, and we find no significant differences between brands. With a bioassay-driven fractionation of liquid smoke, we identified 71 compounds in active fractions by GC-MS and AA spectrometry. However, when these compounds were tested in pure form or in combinations that mimicked the composition of active fractions over a range of concentrations, they failed to stimulate germination to the same degree that smoke fractions did. Moreover, enzymatic oxidation of some of these compounds also failed to stimulate germination. In addition, we tested 43 additional compounds also reported from smoke, 85 compounds that were structurally similar to those reported from smoke and 34 compounds reported to influence germination in other species. Of the 233 compounds tested, 16 proved to inhibit germination at the concentrations tested, and none reproduced the activity of wood smoke. By thermally desorbing smoke produced by cellulose combustions that was trapped on Chromosorb 101, we demonstrate that the cue is desorbed between 125 and 150°C. We estimate that the germination cues are active at concentrations of less than 1 pg/seed and, due to their chromatographic behavior, infer that a number of different chemical structures are active. In separate experiments, we demonstrate that cues remain active for at least 53 days in soil under greenhouse conditions and that the application of aqucous extracts of smoke to soil containing seeds results in dramatic increases in germination of artificial seed banks. Hence, although the chemical nature of the germination cue remains elusive, the stability of the germination cues, their water-solubility, and their activity in low concentrations suggest that these cues could serve as powerful tools for the examination of dormant seed banks and the selective factors thought to be important in the evolution of postfire plant communities. 相似文献
63.
Kim Tak H. Sirdaarta Joseph P. Zhang Qian Eftekhari Ehsan St. John James Kennedy Derek Cock Ian E. Li Qin 《Nano Research》2018,11(4):2204-2216
Nano Research - The toxicity of nanoparticles in a biological system is an integration of effects arising from surface functionality, particle size, ionic dissolution, etc. This complexity suggests... 相似文献
64.
Bennett JC Krishnamoorthy V Liu S Grout RW Hawkes ER Chen JH Shepherd J Pascucci V Bremer PT 《IEEE transactions on visualization and computer graphics》2011,17(12):1822-1831
We present a new framework for feature-based statistical analysis of large-scale scientific data and demonstrate its effectiveness by analyzing features from Direct Numerical Simulations (DNS) of turbulent combustion. Turbulent flows are ubiquitous and account for transport and mixing processes in combustion, astrophysics, fusion, and climate modeling among other disciplines. They are also characterized by coherent structure or organized motion, i.e. nonlocal entities whose geometrical features can directly impact molecular mixing and reactive processes. While traditional multi-point statistics provide correlative information, they lack nonlocal structural information, and hence, fail to provide mechanistic causality information between organized fluid motion and mixing and reactive processes. Hence, it is of great interest to capture and track flow features and their statistics together with their correlation with relevant scalar quantities, e.g. temperature or species concentrations. In our approach we encode the set of all possible flow features by pre-computing merge trees augmented with attributes, such as statistical moments of various scalar fields, e.g. temperature, as well as length-scales computed via spectral analysis. The computation is performed in an efficient streaming manner in a pre-processing step and results in a collection of meta-data that is orders of magnitude smaller than the original simulation data. This meta-data is sufficient to support a fully flexible and interactive analysis of the features, allowing for arbitrary thresholds, providing per-feature statistics, and creating various global diagnostics such as Cumulative Density Functions (CDFs), histograms, or time-series. We combine the analysis with a rendering of the features in a linked-view browser that enables scientists to interactively explore, visualize, and analyze the equivalent of one terabyte of simulation data. We highlight the utility of this new framework for combustion science; however, it is applicable to many other science domains. 相似文献
65.
Direct Method Based Control System for an Autonomous Quadrotor 总被引:1,自引:0,他引:1
Ian D. Cowling Oleg A. Yakimenko James F. Whidborne Alastair K. Cooke 《Journal of Intelligent and Robotic Systems》2010,60(2):285-316
This paper proposes a real time control algorithm for autonomous operation of a quadrotor unmanned air vehicle. The quadrotor
is a small agile vehicle, which as well as being a excellent test bed for advanced control techniques could also be suitable
for internal surveillance, search and rescue and remote inspection. The proposed control scheme incorporates two key aspects
of autonomy; trajectory planning and trajectory following. Using the differentially-flat dynamics property of the system,
the trajectory optimization is posed as a non-linear constrained optimization within the output space in the virtual domain,
not explicitly related to the time domain. A suitable parameterization using a virtual argument as opposed to time is applied,
which ensures initial and terminal constraint satisfaction. The speed profile is optimized independently, followed by the
mapping to the time domain achieved using a speed factor. Trajectory following is achieved with a standard multi-variable
control technique and a digital switch is used to re-optimize the reference trajectory in the event of infeasibility or mission
change. The paper includes simulations using a full dynamic model of the quadrotor demonstrating the suitability of the proposed
control scheme. 相似文献
66.
设计并实现了适用于作战单兵的无线通讯系统,将通常为头盔式或背心式的通讯系统设计为腕带式通讯系统;文中在嵌入式平台上结合单兵通讯系统的要求,提出了腕戴式单兵通讯系统的总体设计;并根据军用要求,通过简化修改TCP/IP协议栈,提出适用于嵌入式的网络协议栈,来实现以单兵通讯系统为基点的局域网组网:选用以Intel Xscale PXA270为处理器的腕式计算机作为单兵数字助理单元以及军用无线电台共同构成单兵无线通讯系统;通过硬件实现完成了两点间的基本通讯功能以及简单的组网过程. 相似文献
67.
68.
69.
The technology profile inventory (TPI) measures attitudes toward computers and the internet. We describe the most recent phase of the construction of the TPI. The studies reported refine and validate the instrument, and we present the final version as an Appendix A. Using a new sample of respondents (N = 394), we replicated the three major factors found previously (Confidence, Approval, and Interest). The TPI scores were related to patterns of information technology (IT) usage and also to gender. To demonstrate the practical utility of the TPI we report (1) results linking TPI scores to behavior during an internet search task; (2) test–retest results obtained as part of a cognitive training experiment using action video games; and (3) results showing that attitudes to IT may be modified by a particular experience with information technology. 相似文献
70.
Liming Lee Kit Ian Kou Wentao Zhang Jinling Liang 《International journal of systems science》2016,47(10):2466-2474
In this paper, we consider finite-time control problems for linear multi-agent systems subject to exogenous constant disturbances and impulses. Some sufficient conditions are obtained to ensure the finite-time boundedness of the multi-agent systems, which could be then reduced to a feasibility problem involving linear matrix inequalities. Numerical examples are given to illustrate the results. 相似文献