Biopreservative potential of Lactobacillus plantarum YML007 and efficacy as a replacement for chemical preservatives in animal feed

Antifungal and biopreservation potentials of Lactobacillus plantarum YML007 isolated from Korean kimchi were analyzed. The biopreservative efficacy of the bacterium was analyzed using maize grains. Maize was divided into 3 groups and treated with a 5× concentrated cell-free supernatant of Lb. plantarum YML007 (T3R3), organic acids (T2R2), and a control group (T1R1) lacking treatment. All groups were stored for 30 days. Maize was tested for moisture and crude fat contents, mold growth, and aflatoxin production. The moisture content, mold count, and toxin production were higher in the control maize. The YML007 and acid treated maize remained uninfected after 30 days. Animals fed with YML007 treated maize showed more weight gain and less feed consumption. YML007 can be used to preserve the nutritional value of stored grain and to ensure better quality feedstuffs that are necessary for improving animal health and performance.     

Heat transfer in a conical cylinder with porous medium

In this paper, numerical study of heat transfer in a conical annular cylinder fixed with saturated porous medium is presented. The heat transfer is assumed to take place by natural convection and radiation. The inner surface of conical cylinder is maintained at uniform wall temperature. The governing partial differential equations are non-dimensionalised using suitable non-dimensional parameters and then solved by using finite element method. The porous medium is divided using triangular elements with uneven element size. A computer software is used to solve the coupled momentum and energy equations in an iterative manner. The results are discussed for various values of geometric and physical parameters of porous medium with emphasis on cone angle of the cylinder. It is seen that the cone angle plays a vital role in heat transfer from the hot surface to porous medium.     

Visualization and quantification of cathode channel flooding in PEM fuel cells

An understanding of two-phase flow mechanisms in micro-channels is critical to water management in fuel cell applications. In this work, an in situ visualization study of cathode flooding in an operating fuel cell is presented. Gas relative humidities of 26%, 42% and 66%, current densities of 0.2, 0.5 and 0.8 A cm⁻²and flow stoichiometries ranging from 2 to 4 are used in this study which represent typical operating conditions for automotive applications. Results are presented in the form of a flow map depicting various two-phase flow patterns. The impact of flooding is also presented in terms of measurable parameters like two-phase pressure drop coefficient and voltage loss. A new parameter called wetted area ratio is introduced to characterize channel flooding and liquid water coverage on a gas diffusion layer, and its repeatability with multiple tests is demonstrated.     

One-step purification of lactoperoxidase from bovine milk by affinity chromatography

Sulphanilamide was determined to be a new inhibitor of lactoperoxidase (LPO) with an IC₅₀ of 0.848.10⁻⁵ M. The K_i for sulphanilamide was determined to be 3.57.10⁻⁵ M and sulphanilamide showed competitive inhibition, which makes it a suitable ligand for constructing a Sepharose 4B-l-tyrosine affinity matrix. The affinity matrix was synthesised by coupling sulphanilamide as the ligand and l-tyrosine as the spacer arm to a cyanogen bromide (CNBr)-activated-Sepharose 4B matrix. Lactoperoxidase was purified 409-fold from the synthesized affinity matrix in a single step, with a yield of 62.3% and a specific activity of 40.9 EU/mg protein. The enzyme activity was measured using ABTS as a chromogenic substrate (pH 6.0). The degree of LPO purification was monitored by SDS–PAGE and its R_z (A₄₁₂/A₂₈₀) value. The R_z value for the purified LPO was found to be 0.7. Maximum binding was achieved and K_m and V_max values were determined.     

Antioxidant Capacity and Phenolic Content of the Aqueous Extract of Commonly Consumed Cucurbits

The antioxidant capacity of commonly consumed cucurbits vegetable was determined by the DPPH, FRAP, Fe³⁺ reducing power, and hydrogen peroxide scavenging assay. The aqueous extract of Luffa cylindrica showed the highest value of phenolic content and antioxidant capacity based on FRAP, Fe³⁺ reducing power, and hydrogen peroxide scavenging assay. However, Laginaria siceraria extract showed the highest flavonoid and DPPH scavenging activities among all three cucurbits used in this study. Phenolic content in aqueous extracts of Luffa cylindrica and Laginaria siceraria was almost equal. Cucurbita maxima exhibited the lowest phenolic, flavonoid content, and exhibited the lowest power of antioxidant scavenging. The antioxidant capacity of cucurbits was significantly correlated (P < 0.05) with the phenolic content of their extracts. The antioxidant capacity of Luffa cylindrica and Laginaria siceraria have also shown a significant correlation (P < 0.05).     

Effects of gating design on structural and mechanical properties of high manganese steel by optimizing casting process parameters

Journal of Mechanical Science and Technology - The impacts of gating design and riser system on structure-property relationship of high manganese steel specimens produced by CO2 sand molding...