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排序方式: 共有477条查询结果,搜索用时 15 毫秒
31.
Y Ichiki S Shimoda H Hara H Shigematsu M Nakamura K Hayashida H Ishibashi Y Niho 《Canadian Metallurgical Quarterly》1997,26(3):728-733
T-cell-mediated autoimmune mechanisms are considered to be involved in the pathogenesis of primary biliary cirrhosis (PBC). In the previous study, we identified the immunodominant T-cell epitope on the E2 component of pyruvate dehydrogenase complex (PDC-E2) in patients with PBC who have HLA-DRB4*0101. In this report, we revealed that the frequency of the T cells reactive to the human PDC-E2 163-176 peptide is significantly increased in the peripheral blood of patients with PBC as compared with healthy subjects. We also confirmed that these T cells were all restricted with HLA-DRB4*01 (DR53) by using HLA-DR-transfected L cells. These results together with the evidence that the immunodominant B-cell epitope overlaps with the human T-cell epitope of the PDC-E2 antigen indicate that the T cells reactive to this epitope are closely associated with the pathogenesis of PBC at least in patients who have HLA-DR53. Therefore, we analyzed the T-cell receptor (TCR) Vbeta sequence of the five different T-cell clones and the three T-cell clones derived from three patients with PBC and healthy subjects, respectively, which are reactive to the human PDC-E2 163-176 peptide in the context of HLA-DR53. The Vbeta- and the Jbeta-gene usages were diverse among the T-cell clones (Vbeta11-Jbeta1.4, Vbeta8-Jbeta1.2, Vbeta12-Jbeta2.1, Vbeta10-Jbeta1.5, and Vbeta20-Jbeta2.1) in patients with PBC. By contrast, in the third complementarity determining region (CDR3), G was frequently found and GXG or GXS motif was identified in all T-cell clones. Moreover, RGXG motif was found in three clones generated from two patients. In healthy subjects, the Vbeta- and the Jbeta-gene usages were also diverse, and GXG and RGXG motif were found. These results indicate that the T cells may recognize the ligand (the human PDC-E2 163-176 peptide/HLA-DR53 complex) using the limited motif in the CDR3 region and that the design of CDR3-specific immunotherapy would be possible using these motifs. 相似文献
32.
P. G. Baranov S. I. Goloshchapov G. A. Medvedkin T. Ishibashi K. Sato 《Journal of Superconductivity》2003,16(1):131-133
Electron paramagnetic (EPR) and ferromagnetic resonance (FMR) have been studied in the system of (Zn,Mn)GeP2 ferromagnetic layer grown on undoped ZnGeP2 single crystal. Strong FMR signals are registered in the wide temperature range up to room temperature. EPR and photo-EPR of intrinsic defects are observed in ZnGeP2 substrate. EPR spectra characteristic of Mn2+ ions on Zn2+ sites in the bulk appear after the growth of the ferromagnetic layer on ZnGeP2 crystal indicating the efficient Mn-diffusion into the bulk crystal by the annealing treatments. 相似文献
33.
Development and applications of an analytical electron microscope with a field emission electron gun
A. Koreeda T. Ishibashi K. Shimizu M. Tomita C. Kimura H. Okano 《Materials Characterization》1990,25(4):375-395
An analytical electron microscope with a field emission electron gun has been developed in order to improve the function of material identification, in order to obtain higher resolution of images, sharper electron diffraction patterns, and purer x-ray spectra with no effects of contamination from smaller areas than in the conventional instruments so far utilized. The performance capabilities of this newly developed analytical electron microscope, the Model H-600FE, have been examined and found to be very useful for the material characterization of nanometer-size areas. Various attributes of the microscope became apparent with reference to the results of different studies. These include better convergence of the electron beam (demonstrated by examination of an MoS2 thin film), high-resolution transmission electron microscopy (using a gold film), high-resolution scanning electron microscopy (with a carbon film containing nanometer-size holes), high-angle resolution electron diffraction (of an iron carbide film), and precision x-ray analysis of nanometer-size areas (using a pyroxene crystal and a Cu---Zn---Al shape memory alloy). 相似文献
34.
Tsukada T. Hashimoto Y. Sakata K. Okada H. Ishibashi K. 《Solid-State Circuits, IEEE Journal of》2005,40(1):67-79
A decoupling circuit using an operational amplifier is proposed to suppress substrate crosstalk in mixed-signal system-on-chip (SoC) devices. It overcomes the parasitic inductance problem of on-chip capacitor decoupling. The effect of the proposed decoupling circuit is not limited by parasitic fine impedance. A 0.13-/spl mu/m CMOS test chip showed that substrate noise at frequencies from 40 MHz to 1 GHz was incrementally suppressed by sequentially activating three of the proposed circuits in parallel. The power dissipation of each circuit was 3.3 mW at a 1.0-V power supply. The test chip measurement showed that the proposed decoupling reduced crosstalk by 31% at 200 MHz, whereas it was reduced by 4.4% with capacitor decoupling. This 7:1 ratio, or 17 dB, corresponds to the gain of the opamp. Design of the opamp and its feedback loop for active decoupling is simple, making the opamp useful for SoC applications. 相似文献
35.
A new three-phase current-fed soft-switching PWM converter is presented. This converter utilises two types of switching commutation scheme to improve the PWM current utilisation rate. It is shown by means of computer simulation that this converter has low THD and offers unity power factor correction 相似文献
36.
Sasaki K. Ishibashi K. Shimohigashi K. Yamanaka T. Moriwake N. Honjo S. Ikeda S. Koike A. Meguro S. Minato O. 《Solid-State Circuits, IEEE Journal of》1990,25(5):1075-1081
A 4-Mb CMOS SRAM having 0.2-μA standby current at a supply voltage of 3 V has been developed. Current-mirror/PMOS cross-coupled cascade sense-amplifier circuits have achieved the fast address access time of 23 ns. A new noise-immune data-latch circuit has attained power-reduction characteristics at a low operating cycle time without access delay. A 0.5-μm CMOS, four-level poly, two-level metal technology with a polysilicon PMOS load memory cell, yielded a small cell area of 17 μm2 and the very small standby current. A quadruple-array, word-decoder architecture allowed a small chip area of 122 mm2 相似文献
37.
16S ribosomal DNA sequences of various strains of Phormidium tenue (P. tenue) in Lake Kamafusa were determined. Such P. tenue could not be distinguished under microscopic examination. From the results of the analyses of base sequences, and using a phylogenetic tree, several P. tenue with different species specificity on the sequence were found in the lake. P. tenue were divided into three groups. The first produced 2-methylisoborneol (MIB) and was close to a species from the moat of Nagoya castle. The second produced MIB and was characteristic of Lake Kamafusa. The third had a brown color like a Lake Biwa strain and was MIB non-producing. From the phylogenetic tree, it was revealed that species had not changed recently by mutation. Moreover, a detection method for P. tenue by PCR and dot hybridization was established. 相似文献
38.
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40.
AlGaAs/GaAs ballistic collection transistors (BCTs) are investigated by self-consistent Monte Carlo simulation, focusing on the space-charge effect in the collector region. In addition to the conventional BCT collector structure (i-p+-n+), modified collector structures which have n--p+-n + and p--p+-n+ doping profiles are examined. By taking account of the fact that the collector delay time is composed of transit time and capacitance charging time, it is shown that the n--p+-n+ collector structure is effective for the suppression of the base-widening effect (Kirk effect) compared to the i-p+-n+ or p- -p+-n+ structure. Donors in the n- layer compensate for the negative space charges produced by near-ballistic electrons. For a simulated BCT with an n--p +-n+ collector, the smaller collector capacitance charging time leads to improvement in current-gain cutoff frequency under high current injection 相似文献