The microscopy cell (MicCell), a versatile modular flowthrough system for cell biology, biomaterial research, and nanotechnology

We describe a novel microfluidic perfusion system for high-resolution microscopes. Its modular design allows pre-coating of the coverslip surface with reagents, biomolecules, or cells. A poly(dimethylsiloxane) (PDMS) layer is cast in a special molding station, using masters made by photolithography and dry etching of silicon or by photoresist patterning on glass or silicon. This channel system can be reused while the coverslip is exchanged between experiments. As normal fluidic connectors are used, the link to external, computer-programmable syringe pumps is standardized and various fluidic channel networks can be used in the same setup. The system can house hydrogel microvalves and microelectrodes close to the imaging area to control the influx of reaction partners. We present a range of applications, including single-molecule analysis by fluorescence correlation spectroscopy (FCS), manipulation of single molecules for nanostructuring by hydrodynamic flow fields or the action of motor proteins, generation of concentration gradients, trapping and stretching of live cells using optical fibers precisely mounted in the PDMS layer, and the integration of microelectrodes for actuation and sensing.     

On the behavior of microstructures with multiple length scales

The current study aims to provide fundamental insight into the behavior of microstructures containing grain sizes that span multiple length scales. A commercial 5083 Al alloy was selected as the material of interest to facilitate comparison with recently published data. The materials studied here were prepared via the thermal consolidation of powders that were cryomilled for different times (i.e., 0, 2, 4, and 8 hours). Following consolidation, the resultant microstructure was characterized by an equiaxed grain morphology with a size distribution centered around 200∼300 nm. Dispersed among the 200- to 300-nm grains were coarse-grained regions or ligaments with a grain size ranging from 600 nm to 2 μm. The occurrence of coarse-grained regions is rationalized on the basis of recrystallization or subgrain coarsening, whereas the occurrence of equiaxed fine regions is proposed to be a result of continuous grain growth. Two types of microstructures were selected for study, containing coarse-grained volumes of approximately 28 pct and 43 pct that corresponded to an ultimate tensile strength (UTS) of 566 MPa and 535 MPa, and a fracture strain of 3.2 pct and 3.5 pct, respectively. The observed ductility and the relevant toughening mechanisms were discussed in light of the presence of multiple length scales.     

Modeling the tensile properties in β-processed α/β Ti alloys

The development of a set of computational tools that permit microstructurally based predictions for the tensile properties of commercially important titanium alloys, such as Ti-6Al-4V, is a valuable step toward the accelerated maturation of materials. This paper will discuss the development of neural network models based on a Bayesian framework to predict the yield and ultimate tensile strengths of Ti-6Al-4V at room temperature. The development of such rules-based model requires the population of extensive databases, which in the present case are microstructurally based. The steps involved in database development include producing controlled variations of the microstructure using novel approaches to heat treatments, the use of standardized stereology protocols to characterize and quantify microstructural features rapidly, and mechanical testing of the heat-treated specimens. These databases have been used to train and test neural network models for prediction of tensile properties. In addition, these models have been used to identify the influence of individual microstructural features on the tensile properties, consequently guiding the efforts toward development of more robust mechanistically based models. Based on the neural network model, it is possible to investigate the influence of individual microstructural features on the tensile properties, and in certain cases these dependencies can point toward unrecognized phenomena. For example, the apparently unexpected trend of increase in tensile strength with increasing prior β-grain size has led to the determination of the pronounced role of the basketweave microstructure in strengthening these alloys, especially in case of larger prior β grains. This article is based on a presentation made in the symposium “Computational Aspects of Mechanical Properties of Materials,” which occurred at the 2005 TMS Annual Meeting, February 13–17, 2005, in San Francisco, CA, under the auspices of the MPMD-Computational Materials Science & Engineering (Jt. ASM-MSCTS) Committee.     

Modeling of anaerobic treatment of wastewater in ponds.

A mathematical model to simulate the performance of anaerobic ponds was developed incorporating both settling of particulate components and the biological anaerobic digestion process. The biological activity includes solubilization of particulate organic matter; methanogenesis and the sulphate reduction process. The model considers that an anaerobic pond comprises a series of equal size columns. Each column has three compartments viz. liquid layer, active sediment layer and inert sediment layer. The existence of organic matter and sulphate removal mechanisms both in the bulk as well as sediment layer of the ponds and the exchange of the soluble components between the layers has been included in the model. The model was transferred to a computer program using VisSim Basic software. The model was verified by comparing simulated results with full-scale as well as with laboratory-scale anaerobic pond performance data. A good agreement between the simulated and the observed pond performance was achieved.     

Multistage Zeolite Drying for Energy-Efficient Drying

This work discusses the potential of three multistage zeolite drying systems (counter-, co-, and cross-current) with a varying number of stages. The evaluation showed that for 2-4 stages with heat recovery the efficiency of the systems ranges between 80 and 90%. Additionally, by introducing a compressor, the latent heat in the exhaust air from the regenerator is recovered and used to heat the inlet air for an additional drying stage. As a result, for the counter-current drying system and compressor pressure 1.5-2 bar, a maximum energy efficiency of 120% is achieved, which results in halving the energy consumption compared to conventional drying systems.     

Guest Editors' Introduction: Attacking Functional Verification through Hybrid Techniques

Hybrid techniques are effective for exploring interesting corner cases, coverage holes, invariant variations, and so forth, in the general area of directed functional validation. However, despite the emergence of several effective hybrid validation techniques, several questions still remain. The five articles in this special issue help explain some of the different facets of this area.     

Modified determination of 2-thiobarbituric acid value in fats and oils

Several methods for the determination of TBA-value were compared with a new direct modification (omitting the previous distillation), using 1-butanol as the sole solvent. The absorbance is measured at 530 nm after heating to 95 degrees C for 120 min, and cooling. The effect of variables was tested by the Youdens ruggedness test (reaction time, temperature, cooling, concentration of sample, thiobarbituric acid, water, heavy metals, antioxidants, chelating agents, bubbling with oxygen or nitrogen). The Lambert-Beers law is valid in the absorbance range between 0.05 and 0.8. The repeatability is better than that of the Dzikowskis procedure and comparable with those of more complicated distillation methods. The standard deviation is in a linear relation with the absorbance (range 0.1-1.0). The method was found satisfactory for evaluating lard, cooking fat, soybean, sunflower, and rapeseed oils in the stage of beginning rancidity.     

A technique for controlling the placement of the thin area in electrojetpolished tem foils of tungsten

Recently we have been making extensive use of TEM observations of grain boundaries in a research program concerned with the deformation behavior of tungsten bicrystals. A major problem in this research involved the preparation of thin foils which contained the grain boundary in the electron tranparent region, since standard cell type electrojetpolishing techniques do not allow adequate control of the placement of the thin area. Since the grain boundary comprises a very small percentage of a foil, good thin area placement control is critical to obtaining useful samples consistently. Masking techniques which use electrically insulating lacquers are not precise enough to guarantee the position of the thin area. However, we have found that one can use the dishing characteristic of the jetpolisher (i.e., the fact that the center of the foil thins faster than the perimeter) in conjunction with the masking technique to predict the position of the thin area within a preferentially thinned region created by the masking procedure outlined below. This is the essence of the procedure outlined below.     

Subjective evaluation of join cost and smoothing methods for unit selection speech synthesis

In unit selection-based concatenative speech synthesis, join cost (also known as concatenation cost), which measures how well two units can be joined together, is one of the main criteria for selecting appropriate units from the inventory. Usually, some form of local parameter smoothing is also needed to disguise the remaining discontinuities. This paper presents a subjective evaluation of three join cost functions and three smoothing methods. We also describe the design and performance of a listening test. The three join cost functions were taken from our previous study, where we proposed join cost functions derived from spectral distances, which have good correlations with perceptual scores obtained for a range of concatenation discontinuities. This evaluation allows us to further validate their ability to predict concatenation discontinuities. The units for synthesis stimuli are obtained from a state-of-the-art unit selection text-to-speech system: rVoice from Rhetorical Systems Ltd. In this paper, we report listeners' preferences for each join cost in combination with each smoothing method.