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101.
T Weaver M Lees V Zaitsev I Zaitseva E Duke P Lindley S McSweeny A Svensson J Keruchenko I Keruchenko K Gladilin L Banaszak 《Canadian Metallurgical Quarterly》1998,280(3):431-442
Primary human osteoblasts were cultured on self-assembled monolayers (SAMs) of alkylthiols on gold with carboxylic acid and methyl termini, and the kinetics of cell attachment and proliferation were measured. Over 90 min approximately twice as many cells attached to carboxylic-acid-terminated monolayers as attached to methyl-terminated monolayers. After 24 h the number of cells attached to carboxylic-acid-terminated monolayers was ten times that attached to the methyl-terminated monolayers. Cell morphology and cytoskeletal actin organization also were found to be different. Osteoblasts were cultured on SAMs that were patterned by photolithographic techniques. Cells attached almost exclusively to carboxylic-acid-functionalized areas of the patterned surfaces, leaving methyl-functionalized regions bare. The patterns strongly influenced the morphology of the attached cells. After 24 h, cells were observed to bridge between carboxylic-acid-terminated regions separated by 75 microns, methyl-terminated regions but not those separated by 150 microns methyl-terminated regions. After 6 days in culture osteoblasts formed multilayers on the carboxylic-acid-terminated regions of the pattern. 相似文献
102.
The cost effectiveness of several modes of family planning service delivery based on the cost per couple-year of protection (CYP), including commodity costs, is assessed for 1991-92 using programme and project data from fourteen developing countries (five in Africa, four in Asia, three in Latin America and two in the Middle East). More than 100 million CYP were provided through these family planning services during the 12 months studied. Sterilisation services provided both the highest volume (over 60% of total) and the lowest cost per CYP ($1.85). Social marketing programmes (CSM), delivering almost 9 million CYPs, had the next lowest cost per CYP on average ($2.14). Clinic-based services excluding sterilisation had an average cost of $6.10. The highest costs were for community-based distribution projects (0.7 million CYPs), which averaged $9.93, and clinic-based services with a community-based distribution component (almost 6 million CYPs), at a cost of $14.00 per CYP. Based on a weighted average, costs were lowest in the Middle East ($3.37 per CYP for all modes of delivery combined) and highest in Africa ($11.20). 相似文献
103.
A new probabilistic paradigm for calculating the uncertainty in casualty area of randomly reentering debris from orbital objects is presented with exemplar calculations of casualty area associated with elements of a reentering Delta II launch vehicle second stage. Sensitivity studies using a probabilistic paradigm for simulation of spacecraft random reentry disassembly have shown the importance of including uncertainties. The sensitivity of casualty area to spacecraft orientation and consequent free-flying ballistic element orientation is extreme. The notion of inherently stochastic processes during reentry is supported by recent data on recovered debris from the Space Shuttle Columbia. Casualty area standards for spacecraft that rely on deterministic estimates may not be providing the level of safety assurance that was intended. 相似文献
104.
Molecular analysis of mammalian timeless 总被引:1,自引:0,他引:1
MJ Zylka LP Shearman JD Levine X Jin DR Weaver SM Reppert 《Canadian Metallurgical Quarterly》1998,21(5):1115-1122
We cloned the mouse cDNA of a mammalian homolog of the Drosophila timeless (tim) gene and designated it mTim. The mTim protein shows five homologous regions with Drosophila TIM. mTim is weakly expressed in the suprachiasmatic nuclei (SCN) but exhibits robust expression in the hypophyseal pars tuberalis (PT). mTim RNA levels do not oscillate in the SCN nor are they acutely altered by light exposure during subjective night. mTim RNA is expressed at low levels in several peripheral tissues, including eyes, and is heavily expressed in spleen and testis. Yeast two-hybrid assays revealed an array of interactions between the various mPER proteins but no mPER-mTIM interactions. The data suggest that PER-PER interactions have replaced the function of PER-TIM dimers in the molecular workings of the mammalian circadian clock. 相似文献
105.
JP Kuehne FA Weaver G Papanicolaou AE Yellin 《Canadian Metallurgical Quarterly》1996,131(9):942-7; discussion 947-8
OBJECTIVE: To assess management of penetrating internal carotid artery (ICA) injuries. DESIGN: Retrospective review of institutional protocol. SETTING: Level 1 trauma center in a major urban area. PATIENTS: Sixty-one patients with penetrating ICA injuries. INTERVENTIONS: In the period 1975 to 1987 (group 1; n = 36), management was based on individual surgeons' preferences. Between 1988 and 1995 (group 2; n = 25), an algorithm was employed: (1) hemodynamically stable patients with suspected ICA injuries underwent a diagnostic angiography; (2) surgically accessible injuries were reconstructed regardless of neurologic status with 2 exceptions: (a) neurologically intact patients with ICA occlusion were treated by anticoagulation and mild pharmacological hypertension and (b) minimal nonocclusive injuries were managed nonoperatively and followed up by serial angiography or duplex ultrasonography; and (3) heparinization, shunting, and completion angiography were employed. MAIN OUTCOME MEASURES: Neurologic status at admission and discharge were compared by the Fisher exact test. RESULTS: In group 1, 24 patients (67%) presented neurologically intact, and 12 (33%) with a deficit. Sixteen injuries were managed nonoperatively, 14 were repaired, and 6 were ligated. At discharge 6 (17%) were improved, 24 (66%) were unchanged, 6 (17%) were worse. Four patients (11%) died of cerebrovascular causes. In group 2, 19 patients (76%) presented neurologically intact, and 6 (24%) with a deficit. Eleven injuries were managed nonoperatively, 12 were repaired, and 2 were ligated. A death occurred in a patient who arrested, was admitted to the hospital in a coma, and died before ICA repair. CONCLUSIONS: Neurologic outcome after ICA injury is enhanced by an algorithm predicated on the liberal use of angiography, a predefined surgical approach, and selective observation. 相似文献
106.
107.
Here we show that the substitution Thr 26-->His in the active site of T4 lysozyme causes the product to change from the alpha- to the beta-anomer. This implies an alteration in the catalytic mechanism of the enzyme. From the change in product, together with inspection of relevant crystal structures, it is inferred that wild-type T4 lysozyme is an anomer-inverting enzyme with a single displacement mechanism in which water attacks from the alpha-side of the substrate. In contrast, the mutant T26H is an anomer-retaining enzyme with an apparently double displacement mechanism in which a water molecule attacks from the opposite side of the substrate. The results also show that the mechanism of wild-type T4 lysozyme differs from that of hen egg-white lysozyme even though both enzymes are presumed to have evolved from a common precursor. 相似文献
108.
According to this review, religion plays a largely positive role in mental health; future research on severe mental disorders should include religious factors more directly. 相似文献
109.
Vandello Joseph A.; Bosson Jennifer K.; Cohen Dov; Burnaford Rochelle M.; Weaver Jonathan R. 《Canadian Metallurgical Quarterly》2008,95(6):1325
The authors report 5 studies that demonstrate that manhood, in contrast to womanhood, is seen as a precarious state requiring continual social proof and validation. Because of this precariousness, they argue that men feel especially threatened by challenges to their masculinity. Certain male-typed behaviors, such as physical aggression, may result from this anxiety. Studies 1-3 document a robust belief in (a) the precarious nature of manhood relative to womanhood and (b) the idea that manhood is defined more by social proof than by biological markers. Study 4 demonstrates that when the precarious nature of manhood is made salient through feedback indicating gender-atypical performance, men experience heightened feelings of threat, whereas similar negative gender feedback has no effect on women. Study 5 suggests that threatening manhood (but not womanhood) activates physically aggressive thoughts. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
110.