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31.
The sensitive detection and characterization of carbohydrates by means of a strategy based on surface-enhanced Raman spectroscopy is demonstrated. Spectra are obtained after injecting a small amount of saccharide solution onto a roughened silver substrate, with subsequent deposition of silver colloid. The sensitivity achieved by this two-step approach enables high-quality Raman spectra to be obtained for small amounts of aqueous saccharides (5 microL of a 10(-2) M solution) utilizing minimal laser power and small signal acquisition times (a few seconds). Spectral "fingerprints" obtained for seven structurally similar monosaccharides demonstrate clearly an effective means by which each sugar can be identified. The application to more complex analyses is demonstrated for monosaccharide mixtures and a disaccharide, whereby the SERS fingerprints aid in the determination of components. 相似文献
32.
In this age of digital impersonation, biometric techniques are being used increasingly as a hedge against identity theft. The premise is that a biometric - a measurable physical characteristic or behavioral trait - is a more reliable indicator of identity than legacy systems such as passwords and PINs. There are three general ways to identify yourself to a computer system, based on what you know, what you have, or who you are. Biometrics belong to the "who you are" class and can be subdivided into behavioral and physiological approaches. Behavioral approaches include signature recognition, voice recognition, keystroke dynamics, and gait analysis. Physiological approaches include fingerprints; iris and retina scans; hand, finger, face, and ear geometry; hand vein and nail bed recognition; DNA; and palm prints. In this article, we focus on the two most popular biometric techniques: fingerprints and iris scans. 相似文献
33.
SA Lelièvre VM Weaver JA Nickerson CA Larabell A Bhaumik OW Petersen MJ Bissell 《Canadian Metallurgical Quarterly》1998,95(25):14711-14716
What determines the nuclear organization within a cell and whether this organization itself can impose cellular function within a tissue remains unknown. To explore the relationship between nuclear organization and tissue architecture and function, we used a model of human mammary epithelial cell acinar morphogenesis. When cultured within a reconstituted basement membrane (rBM), HMT-3522 cells form polarized and growth-arrested tissue-like acini with a central lumen and deposit an endogenous BM. We show that rBM-induced morphogenesis is accompanied by relocalization of the nuclear matrix proteins NuMA, splicing factor SRm160, and cell cycle regulator Rb. These proteins had distinct distribution patterns specific for proliferation, growth arrest, and acini formation, whereas the distribution of the nuclear lamina protein, lamin B, remained unchanged. NuMA relocalized to foci, which coalesced into larger assemblies as morphogenesis progressed. Perturbation of histone acetylation in the acini by trichostatin A treatment altered chromatin structure, disrupted NuMA foci, and induced cell proliferation. Moreover, treatment of transiently permeabilized acini with a NuMA antibody led to the disruption of NuMA foci, alteration of histone acetylation, activation of metalloproteases, and breakdown of the endogenous BM. These results experimentally demonstrate a dynamic interaction between the extracellular matrix, nuclear organization, and tissue phenotype. They further show that rather than passively reflecting changes in gene expression, nuclear organization itself can modulate the cellular and tissue phenotype. 相似文献
34.
Dynamic strain aging has been investigated at temperatures between 77 and 1100 K in eight polycrystalline NiAl alloys. The 0.2% offset yield stress and work hardening rates for these alloys generally decreased with increasing temperature. However, local plateaus or maxima were observed in conventional purity and carbon doped alloys at intermediate temperatures (600–900 K). This anomalous behavior was not observed in low interstitial high-purity, nitrogen doped, or in titanium doped materials. Low or negative strain rate sensitivities (SRS) were also observed in all eight alloys in this intermediate temperature range. Coincident with the occurrence of negative SRS was the occurrence of serrated flow in conventional purity alloys containing high concentrations of Si in addition to C. These phenomena have been attributed to dynamic strain aging (DSA). Chemical analysis of the alloys used in this study suggests that the main species causing strain aging in polycrystalline NiAl is C but indicate that residual Si impurities can enhance the strain aging effect. 相似文献
35.
J. W. Dickens T. B. Whitaker R. J. Monroe J. N. Weaver 《Journal of the American Oil Chemists' Society》1979,56(9):842-844
A subsampling mill was constructed with 19 subsample spouts similar to the 1 or 2 subsample spouts on the conventional subsampling mills. Samples of peanuts, each of which contained 1 kernel made radioactive by neutron activation, were comminuted in the mill. For treatment M the discharge from each spout was kept separate. For treatment R, the discharge from all 19 spouts was blended together in a twin-shelled blender and subdivided with a riffle divider into 16 subsamples which averaged the same weight as the M subsamples. Radioactivity counts/gram of each subsample were measured. Eleven samples were comminuted for each treatment which produced a total of 209 M samples and 176 R samples. An analysis of variance on the pooled data from each treatment showed a mean square error of 1318.3 for the M treatment and 1278.6 for the R treatment. The null hypothesis that the mean square errors for the two treatments are equal was not rejected by the F test (P=0.421). 相似文献
36.
37.
Vanessa Sanchez Kausalya Mahadevan Gabrielle Ohlson Moritz A. Graule Michelle C. Yuen Clark B. Teeple James C. Weaver James McCann Katia Bertoldi Robert J. Wood 《Advanced functional materials》2023,33(26):2212541
Soft robots adapt passively to complex environments due to their inherent compliance, allowing them to interact safely with fragile or irregular objects and traverse uneven terrain. The vast tunability and ubiquity of textiles has enabled new soft robotic capabilities, especially in the field of wearable robots, but existing textile processing techniques (e.g., cut-and-sew, thermal bonding) are limited in terms of rapid, additive, accessible, and waste-free manufacturing. While 3D knitting has the potential to address these limitations, an incomplete understanding of the impact of structure and material on knit-scale mechanical properties and macro-scale device performance has precluded the widespread adoption of knitted robots. In this work, the roles of knit structure and yarn material properties on textile mechanics spanning three regimes–unfolding, geometric rearrangement, and yarn stretching–are elucidated and shown to be tailorable across unique knit architectures and yarn materials. Based on this understanding, 3D knit soft actuators for extension, contraction, and bending are constructed. Combining these actuation primitives enables the monolithic fabrication of entire soft grippers and robots in a single-step additive manufacturing procedure suitable for a variety of applications. This approach represents a first step in seamlessly “printing” conformal, low-cost, customizable textile-based soft robots on-demand. 相似文献
38.
WI Bensinger KS Schiffman L Holmberg FR Appelbaum R Maziarz P Montgomery E Ellis S Rivkin P Weiden K Lilleby S Rowley S Petersdorf JP Klarnet W Nichols A Hertler R McCroskey CH Weaver CD Buckner 《Canadian Metallurgical Quarterly》1997,19(12):1183-1189
The purpose of this study was to determine the outcome of patients with metastatic breast cancer treated with high-dose busulfan (Bu), melphalan (Mel) and thiotepa (TT) followed by peripheral blood stem cell (PBSC) infusion. Fifty-one patients with chemotherapy refractory (n = 32) or responsive (n = 19) metastatic breast cancer received Bu (12 mg/kg), Mel (100 mg/m2) and TT (500 mg/m2) followed by PBSC collected after chemotherapy and growth factor (n = 43) or growth factor alone (n = 8). The 100 day treatment-related mortality was 8% including one death from cytomegalovirus pneumonia, one from aspiration pneumonia and two from regimen-related toxicity (RRT). Seven of 28 refractory (25%) and 5/7 (71%) responsive patients with evaluable disease achieved a complete response of all measurable disease or all soft tissue disease with at least improvement in bone lesions (PR*). Fifteen of 51 patients (29%) are alive and progression-free a median of 423 days (range 353-934) after treatment, 5/32 (16%) with refractory disease and 10/19 (53%) with responsive disease. The probabilities of progression-free survival (PFS) at 1.5 years for the patients with refractory (n = 32) and responsive (n = 19) disease were 0.24 and 0.53, respectively. These preliminary data suggest that high-dose Bu/Mel/TT has significant activity in patients with advanced breast cancer and may be superior to some previously published regimens. 相似文献
39.
Lynnette D. Madsen Louise Weaver Henrik Ljungcrantz Alison J. Clark 《Journal of Electronic Materials》1998,27(5):418-426
The microstructure of the Pt/Ti/SiO2/Si structure has been investigated by scanning and transmission electron microscopy. Pt films of 100 nm thickness deposited
by sputtering or evaporation onto unheated substrates gave complete coverage of the underlying Ti layer and showed a granular
and faceted structure with grains ∼20 nm in diameter. They did not exhibit hillocks or surface TiOx formation. X-ray diffraction was used to examine the film stress through use of the sin2ψ method with bulk values for the elastic constants (v=0.39, E=162 GPa). The as-deposited sputtered film had a compressive
stress of ∼540 MPa, while the evaporated films had tensile stresses of ∼630 MPa. The films then received a 400°C rapid thermal
anneal (RTA) for 90 s and a subsequent RTA of 650°C for 30s. Further investigation of the film stresses and microstructure
were made after each annealing step. After the low temperature anneal, the film stress for the sputtered film became tensile.
Plan-view sections examined by transmission electron microscopy (TEM) showed that the as-deposited sputtered films were dense
but became porous after annealing. Initially, the evaporated films had a less dense microstructure, but were more stable with
annealing. Little change in the stress for the evaporated film was observed after this initial low temperature annealing step.
Additional annealing of the evaporated and sputtered samples caused complete consumption of the Ti layer including some TiOx formation from the underlying SiO2 layer and marked interaction with the Pt; however, little change in the stress was found. The surface of the Pt film revealed
larger grains, but otherwise remained unaffected. The underlying phase changes were minimized once the Ti layer had reacted
with the Pt. Due to the ratio of the layers, Pt:Ti of 2:1, the surface of the Pt was unaffected. 相似文献
40.
M. C. Freeman G. R. Buell L. E. Hay W. B. Hughes R. B. Jacobson J. W. Jones S. A. Jones J. H. Lafontaine K. R. Odom J. T. Peterson J. W. Riley J. S. Schindler C. Shea J. D. Weaver 《河流研究与利用》2013,29(7):906-918
Efforts to conserve stream and river biota could benefit from tools that allow managers to evaluate landscape‐scale changes in species distributions in response to water management decisions. We present a framework and methods for integrating hydrology, geographic context and metapopulation processes to simulate effects of changes in streamflow on fish occupancy dynamics across a landscape of interconnected stream segments. We illustrate this approach using a 482 km2 catchment in the southeastern US supporting 50 or more stream fish species. A spatially distributed, deterministic and physically based hydrologic model is used to simulate daily streamflow for sub‐basins composing the catchment. We use geographic data to characterize stream segments with respect to channel size, confinement, position and connectedness within the stream network. Simulated streamflow dynamics are then applied to model fish metapopulation dynamics in stream segments, using hypothesized effects of streamflow magnitude and variability on population processes, conditioned by channel characteristics. The resulting time series simulate spatially explicit, annual changes in species occurrences or assemblage metrics (e.g. species richness) across the catchment as outcomes of management scenarios. Sensitivity analyses using alternative, plausible links between streamflow components and metapopulation processes, or allowing for alternative modes of fish dispersal, demonstrate large effects of ecological uncertainty on model outcomes and highlight needed research and monitoring. Nonetheless, with uncertainties explicitly acknowledged, dynamic, landscape‐scale simulations may prove useful for quantitatively comparing river management alternatives with respect to species conservation. Published 2012. This article is a U.S. Government work and is in the public domain in the USA. 相似文献