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51.
52.
We have previously observed that bovine papillomavirus type 1 (BPV-1) induces the appearance of five cellular proteins in C127 mouse fibroblasts, four of which appear to arise by altered processing of resident endoplasmic reticulum proteins. Studies of various cell lines revealed that expression of the 3' end of the BPV early region was sufficient for induction of these changes. To identify the BPV gene responsible, we have utilized the simian virus 40 (SV40)/BPV-1 recombinant virus Pava-1, which expresses the 3' end of the BPV early region behind an SV40 early promoter. C127 cells infected with Pava-1 for 48 h show the expected BPV-associated alterations, as do cells infected with Pava constructs mutated in the E5 or E2 genes. However, a mutation in the start codon of a previously ignored open reading frame extending from nucleotides 4013 to 4170 (E5B) eliminated the BPV-associated changes. Similar results were obtained with COS cells infected with the Pava mutants and C127 cells transformed by full-length mutated BPV. Despite its influence on the processing of cellular endoplasmic reticulum proteins, this mutation in E5B did not alter BPV-transforming efficiency or the ability of transformants to form colonies in soft agar. The E5B open reading frame encodes a hydrophobic 52-amino-acid polypeptide that shares structural similarities with HPV6 E5A and HPV16 E5. Speculations on a role for E5B in the viral life cycle are discussed. 相似文献
53.
EM de Jong BA Seegers MK Gulinck JB Boezeman PC van de Kerkhof 《Canadian Metallurgical Quarterly》1996,193(4):300-303
We present the first case of esophagogastric devascularization and esophagogastric transection using a stapler through laparoscopic surgery. The procedure was performed in a 71-year-old diabetic woman with alcoholic liver cirrhosis (Child-Pugh B class), portal hypertension, bleeding grade III esophageal varices, and a previous bleeding episode. The surgical technique was carried out without problems, and the patient had an excellent postoperative condition. Esophagogastric devascularization with esophageal transection using a stapler through laparoscopic surgery is a feasible technique that accomplishes the same and all objectives of the open procedure. Operative time in both methods is the same, whereas surgical trauma, inmunologic depletion, amount of transfused blood, pain, use of analgesics, and hospital stay are reduced in the laparoscopic technique. 相似文献
54.
In the present study, the effect of bradykinin on basal and precontracted mouse-isolated trachea was investigated. In basal conditions mouse-isolated tracheal rings do not respond to bradykinin. However, when the tracheal rings were precontracted with carbachol (10(-7) M) a relaxation with bradykinin (3 x 10(-9)-3 x 10(-7)) was found. The maximal response amounted 69.7+/-4.1% (n=15) with a pD2 value of 7.2+/-0.21. The selective bradykinin B2 receptor antagonist HOE 140 (10(-10)-10(-8) M) antagonized the bradykinin-induced relaxation, while the bradykinin B1 receptor antagonist des-Arg9-Leu8-bradykinin (10(-6) M) had no influence. The selective bradykinin B1 receptor agonist des-Arg9-bradykinin (10(-6) M) caused a small relaxation (8.4+/-2.5%, n=6), which could be antagonized completely by the selective bradykinin B1 receptor antagonist des-Arg9-Leu8-bradykinin (10(-6) M) while addition of the selective bradykinin B2 receptor antagonist HOE 140 (10(-8) M) was without effect. In the presence of indomethacin (10(-6) M) the relaxation of bradykinin was completely abolished. Pretreatment of the tracheal rings with capsaicin, or the presence of the selective NK1 receptor antagonist RP 67851 (10(-6) M) or the presence of the nitric oxide synthase inhibitor L-NAME (3 x 10(-4) M) had no effect on the bradykinin-induced relaxation. In conclusion, these results demonstrate that the mouse-isolated tracheal is a preparation in which bradykinin exerts a relaxant response via stimulation of bradykinin B2 receptors. This response is probably mediated by prostaglandins. 相似文献
55.
Xinjian Duan Mukesh Jain David S. Wilkinson 《Metallurgical and Materials Transactions A》2006,37(12):3489-3501
A heterogeneous finite element model with randomly distributed inhomogeneities has been developed for the determination of
the forming limit diagram (FLD) for thin aluminum sheet material based on the prediction of localized necking. The strength
difference between the inhomogeneities and the matrix is ascertained either from the fluctuation of the experimental stress-strain
curve or from a micromechanical analysis that uses a representative particle field. By changing the specimen geometry and
friction conditions, different stress states (or strain paths) are achieved. A plot of the critical Oyane fracture parameter
is used to identify the limit strain state. Also, a plot of equivalent plastic strain rate is used to distinguish the boundary
of intense shear bands and hence to identify where to take the measurement point. Both a plane stress model and a three-dimensional
(3-D) model are adopted to predict the shear banding phenomenon and hence the FLD. The predicted FLD agrees well with the
measurements from a recent round robin experimental FLD involving several independent research laboratories. The Taguchi method
is applied to assess how the various parameters involved in the heterogeneous model affect the calculated forming limit strain. 相似文献
56.
57.
M. Jain 《Journal of Materials Science》1992,27(2):399-407
The evolution of dislocation structure during room-temperature, uniaxial, low-cycle fatigue of an overaged Al-Mg-Si alloy is studied. Ageing at 450°C produces a fine dispersion of Mg2Si precipitate particles. During fully reversed strain-controlled cyclic tests, these fine particles restrict deformation to local regions and a stable dislocation substructure is developed early in fatigue life. Substructural observations of hardening and saturation by transmission electron microscopy reveal extensive dislocation band formation on Mg2Si precipitate rods. Various microstructural features such as configuration of tangled dislocations, dislocation cells, precipitate morphologies, sizes, precipitate-free zones, etc., have been examined during cyclic hardening and saturation. The results have been analysed in terms of kinematic and isotropictype microstructural mechanisms. 相似文献
58.
470 injured teeth of 370 patients who consulted the Dental Clinic of Ege University, Izmir, Turkey for examination or treatment between 1981-1993 were evaluated. Information concerning sex, age of patients at the time of injury, cause of trauma, number of injured teeth, type of tooth and type of trauma were recorded. More boys suffered traumatic injuries (64.8%) than girls (35.2%). Patients aged 11-15 years old exhibited the highest number of injuries (34.4%) followed by the 6-10 years old group (24.5%). Most injuries involved one tooth (60%) and maxillary central incisors were the most often affected teeth (66.2%). The leading cause of injury was undefined falls (45.1%). At the initial examination, cases seen after a long posttraumatic period showed more complications than those presented within a short time period. Educational programs about the importance of dental trauma, the benefits of immediate attendance and conservation of avulsed and fractured teeth would be very helpful for patients. Additionally improving the knowledge of the dental practitioner about trauma would be another important point in solving the problem. 相似文献
59.
Plastic instability in dual-pressure tube-hydroforming process 总被引:1,自引:0,他引:1
The tube-hydroforming process has become an indispensable manufacturing technique in recent years. Successful tube hydroforming requires bulging to take place without causing any type of instability such as bursting, wrinkling or buckling. The dual-pressure tube-hydroforming process was introduced to achieve a favorable tri-axial stress state in the deformation process. In this paper, the effect of applying counter pressure on plastic instability of thin-walled tubes is analyzed. It is concluded that in dual-pressure tube hydroforming, the onset of plastic instability is delayed and the ductility of the metal is increased. 相似文献
60.
Developing retinal axons must follow a stereotypic course directing them to the subcortical visual centres which on arrival they must recognise. Transplantation studies suggest that local substrate cues close to the surface of the brainstem and diffusible factors emanating from the target region are important. To test a role for diffusible factors, we transplanted retinae to the cerebral cortex and have shown that outgrowth can be promoted by BDNF secreting fibroblasts. 相似文献