Rolling the dice: multidimensional visual exploration using scatterplot matrix navigation

Scatterplots remain one of the most popular and widely-used visual representations for multidimensional data due to their simplicity, familiarity and visual clarity, even if they lack some of the flexibility and visual expressiveness of newer multidimensional visualization techniques. This paper presents new interactive methods to explore multidimensional data using scatterplots. This exploration is performed using a matrix of scatterplots that gives an overview of the possible configurations, thumbnails of the scatterplots, and support for interactive navigation in the multidimensional space. Transitions between scatterplots are performed as animated rotations in 3D space, somewhat akin to rolling dice. Users can iteratively build queries using bounding volumes in the dataset, sculpting the query from different viewpoints to become more and more refined. Furthermore, the dimensions in the navigation space can be reordered, manually or automatically, to highlight salient correlations and differences among them. An example scenario presents the interaction techniques supporting smooth and effortless visual exploration of multidimensional datasets.     

Biomarker analysis of stored blood products: emphasis on pre-analytical issues

Millions of blood products are transfused every year; many lives are thus directly concerned by transfusion. The three main labile blood products used in transfusion are erythrocyte concentrates, platelet concentrates and fresh frozen plasma. Each of these products has to be stored according to its particular components. However, during storage, modifications or degradation of those components may occur, and are known as storage lesions. Thus, biomarker discovery of in vivo blood aging as well as in vitro labile blood products storage lesions is of high interest for the transfusion medicine community. Pre-analytical issues are of major importance in analyzing the various blood products during storage conditions as well as according to various protocols that are currently used in blood banks for their preparations. This paper will review key elements that have to be taken into account in the context of proteomic-based biomarker discovery applied to blood banking.     

Image- and Fluorescence-Based Test Shows Oxidant-Dependent Damages in Red Blood Cells and Enables Screening of Potential Protective Molecules

An increase of oxygen saturation within blood bags and metabolic dysregulation occur during storage of red blood cells (RBCs). It leads to the gradual exhaustion of RBC antioxidant protective system and, consequently, to a deleterious state of oxidative stress that plays a major role in the apparition of the so-called storage lesions. The present study describes the use of a test (called TSOX) based on fluorescence and label-free morphology readouts to simply and quickly evaluate the oxidant and antioxidant properties of various compounds in controlled conditions. Here, TSOX was applied to RBCs treated with four antioxidants (ascorbic acid, uric acid, trolox and resveratrol) and three oxidants (AAPH, diamide and H₂O₂) at different concentrations. Two complementary readouts were chosen: first, where ROS generation was quantified using DCFH-DA fluorescent probe, and second, based on digital holographic microscopy that measures morphology alterations. All oxidants produced an increase of fluorescence, whereas H₂O₂ did not visibly impact the RBC morphology. Significant protection was observed in three out of four of the added molecules. Of note, resveratrol induced diamond-shape “Tirocytes”. The assay design was selected to be flexible, as well as compatible with high-throughput screening. In future experiments, the TSOX will serve to screen chemical libraries and probe molecules that could be added to the additive solution for RBCs storage.