全文获取类型
收费全文 | 259篇 |
免费 | 0篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 1篇 |
化学工业 | 56篇 |
金属工艺 | 4篇 |
机械仪表 | 4篇 |
建筑科学 | 15篇 |
能源动力 | 15篇 |
轻工业 | 12篇 |
水利工程 | 1篇 |
无线电 | 34篇 |
一般工业技术 | 52篇 |
冶金工业 | 11篇 |
原子能技术 | 5篇 |
自动化技术 | 50篇 |
出版年
2023年 | 1篇 |
2021年 | 12篇 |
2020年 | 4篇 |
2019年 | 4篇 |
2018年 | 1篇 |
2017年 | 4篇 |
2016年 | 7篇 |
2014年 | 5篇 |
2013年 | 14篇 |
2012年 | 14篇 |
2011年 | 25篇 |
2010年 | 15篇 |
2009年 | 16篇 |
2008年 | 22篇 |
2007年 | 16篇 |
2006年 | 17篇 |
2005年 | 6篇 |
2004年 | 5篇 |
2003年 | 10篇 |
2002年 | 3篇 |
2001年 | 4篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1995年 | 4篇 |
1993年 | 3篇 |
1992年 | 2篇 |
1991年 | 6篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 3篇 |
1985年 | 2篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1979年 | 5篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1970年 | 3篇 |
1968年 | 1篇 |
排序方式: 共有260条查询结果,搜索用时 31 毫秒
71.
Deglane G Abes S Michel T Prévot P Vives E Debart F Barvik I Lebleu B Vasseur JJ 《Chembiochem : a European journal of chemical biology》2006,7(4):684-692
The grafting of cationic groups to synthetic oligonucleotides (ONs) in order to reduce the charge repulsion between the negatively charged strands of a duplex or triplex, and consequently to increase a complex's stability, has been extensively studied. Guanidinium groups, which are highly basic and positively charged over a wide pH range, could be an efficient ON modification to enhance their affinity for nucleic acid targets and to improve cellular uptake. A straightforward post-synthesis method to convert amino functions attached to ONs (on sugar, nucleobase or backbone) into guanidinium tethers has been perfected. In comparison to amino groups, such cationic groups anchored to alpha-oligonucleotide phosphoramidate backbones play important roles in duplex stability, particularly with RNA targets. This high affinity could be explained by dual recognition resulting from Watson-Crick or Hoogsteen base pairing combined with cationic/anionic backbone recognition between strands involving H-bond formation and salt bridging. Molecular-dynamics simulations corroborate interactions between the cationic backbones of the alpha-ONs and the anionic backbones of the nucleic acid targets. Moreover, ONs with guanidinium modification increased cellular uptake relative to negatively charged ONs. The cellular localization of these new cationic phosphoramidate ONs is mainly cytoplasmic. The uptake of these ON analogues might occur through endocytosis. 相似文献
72.
The processes of mass transfer in the material influence not only the conditions within the material but also inside the connected air spaces. A new module for precise representation of mass transfer in materials in contact with the indoor air, called Humi-mur, was elaborated and validated in this work. It allows for the precise representation of sorption isotherm and vapour permeability dependence on relative humidity. Also the sorption curve hysteresis has been implemented. The new module was then applied to estimate the sensitivity of the results to uncertainty in measured material properties and the impact of hysteresis effect. Reasonable estimation of experimental uncertainty resulted in the deviation of approximately 6% in the calculated results. Hysteresis quite strongly influences the dynamic behaviour of materials. Concerning hysteresis in the sorption isotherm, we showed that the average of the adsorption and desorption equations is a reasonable approximation of mean behaviour for coarse calculation. In case when precise results of the relative humidity (absolute humidity) are needed, the hysteresis effect should be taken into account. 相似文献
73.
Frédéric Kuznik Christian Obrecht Gilles Rusaouen Jean-Jacques Roux 《Computers & Mathematics with Applications》2010,59(7):2380-2392
Graphics Processing Units (GPUs), originally developed for computer games, now provide computational power for scientific applications. In this paper, we develop a general purpose Lattice Boltzmann code that runs entirely on a single GPU. The results show that: (1) simple precision floating point arithmetic is sufficient for LBM computation in comparison to double precision; (2) the implementation of LBM on GPUs allows us to achieve up to about one billion lattice update per second using single precision floating point; (3) GPUs provide an inexpensive alternative to large clusters for fluid dynamics prediction. 相似文献
74.
Suéry Michel 《稀有金属(英文版)》2006,25(4):377-381
The effects of aging treatment on the microstructures and mechanical properties of extruded AM50 + xCa alloys (x=0, 1, 2 wt.%) were studied. The results indicated the secondary phase Mgl7Al12 precipitated from the saturated α-Mg solid solution while Al2Ca changed slightly when the aging time was increased. The hardness of extruded AM50 + xCa al- loys increased initially to its peak, and then dropped to reach its original hardness with the increase in aging time. With the increase in aging temperature, the hardness of the AM50 + 2Ca ahoy decreased, whereas the hardness of AM50 and AM50 + 1Ca alloys decreased in the initial stages of aging treatment and increased in the later stages of aging treatment. The tensile strengths of AM50 and AM50 + 1Ca alloys increased after aging treatment for the precipitation of Mg17Al12 phase, which increases the resistance against dislocation movement at the grain boundary; with increase in aging temperature, their tensile strengths increased. For AM50 + 2Ca alloy, the tensile strength declined after aging at 150℃ and 175℃, while it increased slightly at 200℃. The ductility of AM50 + xCa alloys (x = 0, 1, 2 wt.%) declined after aging treatment. 相似文献
75.
Joffraud JJ Cardinal M Cornet J Chasles JS Léon S Gigout F Leroi F 《International journal of food microbiology》2006,112(1):51-61
Cold-smoked salmon is a lightly preserved fish product in which a mixed microbial flora develops during storage and where the interactive behaviour of micro-organisms may contribute to their growth and spoilage activity. The aim of this study was to assess the effect of the bacterial interactions between the main species contaminating the cold-smoked salmon on bacterial growth, chemical and sensory changes, and spoilage. First, Carnobacterium piscicola, Photobacterium phosphoreum, Lactobacillus sakei, Vibrio sp., Brochothrix thermosphacta and Serratia liquefaciens-like were inoculated as pure cultures on sterile cold-smoked salmon. All bacterial species grew well; Vibrio sp. was the fastest and L. sakei strains developed very rapidly as well with a high maximum cell density on cold-smoked salmon blocks (up to 10(9) cfu g(-1) after 10 days at 8 degrees C). Based on sensory analysis, Vibrio sp. was identified as non-spoilage bacteria, C. piscicola as very lightly and B. thermosphacta as lightly spoiling. L. sakei and S. liquefaciens-like were found to be the most spoiling bacteria. Secondly, C. piscicola and L. sakei, two species frequently occurring in the lactic flora of the product, were inoculated together and each of them in mixed cultures with respectively P. phosphoreum, Vibrio sp., B. thermosphacta, and S. liquefaciens-like. The growth of L. sakei was shown to strongly inhibit most of the co-inoculated strains i.e. P. phosphoreum, B. thermosphacta, S. liquefaciens-like and, to a lesser extent, Vibrio sp. The growth of C. piscicola seemed to be enhanced with B. thermosphacta and to develop earlier with P. phosphoreum and Vibrio sp. Conversely, S. liquefaciens-like and P. phosphoreum were weakly inhibited by C. piscicola. The main observation resulting from the sensory evaluation was the delay in the appearance of the spoilage characteristics in the mixed cultures with L. sakei, in particular L. sakei/ S. liquefaciens-like. On the other hand, the spoilage activity of the non-spoiler strains Vibrio sp. or the moderate spoilage strains B. thermosphacta and C. piscicola was increased when they were associated together. It is concluded that the spoilage behaviour of micro-organisms in mixed culture is significantly different from pure culture and explain the difficulty to find robust quality indices for this product. 相似文献
76.
The helicoidal vortex model developed by the author is compared with data of the National Renewable Energy Laboratory (NREL) Unsteady Aerodynamics Experiment (UAE). The B Sequence has been used for this purpose. It corresponds to the “downwind configuration” where the blades cross the tower shadow. Several cases have been analyzed with wind speeds of V = 5, 7, 10 and 12 m/s and yaw angles of up to 30°. The results indicate that the modelization of the tower shadow gives good results in the range of applicability of the vortex model, at a low computer cost, and demonstrates further the versatility of this approach. 相似文献
77.
A procedure to estimate a two-component mixture model where one component is known is proposed. The unknown part is estimated with a weighted kernel function. The weights are defined in an adaptive way. The convergence to a unique solution of our estimation procedure is proven. The procedure is compared with two classical approaches using simulation. In addition, the results obtained are applied to multiple testing procedure in order to estimate the posterior population probabilities and the local false discovery rate. 相似文献
78.
The adrenal cortex is a highly vascularized endocrine tissue. A dense network of blood capillaries centripetally irrigates the adrenal gland, allowing every endocrine cell to be in contact with an endothelial cell. The pituitary hormone ACTH controls the coordinated development of the vasculature and the endocrine tissue mass. This suggests that paracrine secretions between steroidogenic adrenocytes and capillary endothelial cells participate in the control of adrenocortical homeostasis. Besides its effect on the vascular tone of arteries, ACTH induces the expression of the angiogenic cytokine VEGF-A (vascular endothelial growth factor-A) in primary cultures of adrenocortical cells. This growth factor is a specific mitogen for endothelial cells and is likely to mediate the hormonal control of adrenocortical vascularization through a paracrine mechanism. The newly discovered angiogenic factor EG-VEGF (endocrine-gland-derived vascular endothelial growth factor), the expression of which is restricted to endocrine glands and which is preferentially mitogenic for endocrine tissue-derived endothelial cells, is another candidate mediator of great potential interest. 相似文献
79.
Pierrat R Greffet JJ Carminati R Elaloufi R 《Journal of the Optical Society of America. A, Optics, image science, and vision》2005,22(11):2329-2337
We study the spatial coherence of an optical beam in a strongly scattering medium confined in a slab geometry. Using the radiative transfer equation, we study numerically the behavior of the transverse spatial coherence length in the different transport regimes. Transitions from the ballistic to the diffusive regimes are clearly identified. 相似文献
80.
Chen W Poullet E Burie J Boucher D Sigrist MW Zondy JJ Isaenko L Yèlisseyev A Lobanov S 《Applied optics》2005,44(19):4123-4129
The first demonstration, to the best of our knowledge, of continuous-wave (cw) difference-frequency generation (DFG) in LiInS2 crystal is reported. Wide spectral coverage (5.5-11.3 microm) has been obtained with angle and wavelength tuning for type II (eoe) critically phase-matched parametric interaction. The phase-matching conditions in cw DFG have been investigated, which allowed us to improve the Sellmeier parameters by use of a two-pole dispersion equation. An effective nonlinear coefficient deff = 6.9 +/- 0.8 pm/V has been determined at approximately 7 microm relative to the well-known nonlinear coefficient d36 of AgGaS2, which yields a power-conversion efficiency of approximately 12.4 microW/(W2 cm). We evaluated the high-resolution spectral characteristics of the DFG source by recording C2H2 and SO2 spectra. 相似文献