Improvement of measurement techniques for damping induced by micro-sliding

This paper deals with the damping caused by friction in joints. A new test bench is presented and justified by comparisons made with devices described in the literature. The purpose of this academic bench is to measure the damping induced by partial slip and friction in a planar joint. Moreover, allows uncoupling normal static and dynamic tangential forces. A new method for so-called stopped-sine excitation was developed. It allows more precise monitoring of the evolution of the vibration frequency and damping of non-linear modes. This method is associated with piezoelectric exciters for greater efficiency when stopping excitation. A large number of experimental results are presented and discussed. They are used to characterize the damping induced by micro-sliding in the bonds.     

Behavior of RC columns strengthened with different CFRP systems under eccentric loading

The present study investigates the performance of eccentrically loaded columns externally strengthened with different carbon fiber-reinforced polymer (CFRP) systems. The 10 specimens were representative-scale square columns made of normal-strength concrete with substandard (internal) reinforcement details that were designed to represent old building structural columns. Eight columns were upgraded by four types of commercially available systems of external reinforcement, using plates, unidirectional or bi-directional composite fabrics. It was considered necessary to get information on a wide spectrum of carbon fiber-reinforcement systems in order to provide a satisfactory set of experimental data for validating future suitable retrofitting design methods. Experimental results presented in this paper show that a significant improvement of the strength capacity, deformation capacity and ductility of columns can result of the CFRP application, but the observed gains strongly depend on the reinforcement systems.     

Additive Composition of Supervised Self-Organizing Maps

The learning of complex relationships can be decomposed into several neural networks. The modular organization is determined by prior knowledge of the problem that permits to split the processing into tasks of small dimensionality. The sub-tasks can be implemented with neural networks, although the learning examples cannot be used anymore to supervise directly each of the networks. This article addresses the problem of learning in a modular context, developing in particular additive compositions. A simple rule allows defining efficient training, and combining, for example, several Supervised-SOM networks. This technique is important because it introduces interesting generalizations in many modular compositions, permitting data fusion or sequential combinations of neural networks. This revised version was published online in August 2006 with corrections to the Cover Date.     

Species identification of meat products by ELISA

ELISA methods used in this study are proved to detect low contents of animal species (pork, beef, sheep and poultry), even in highly processed foods. They present the advantages of being robust, cheap and easy to perform. Nevertheless, F factors, determining the threshold values of the test, need to be validated for each species.     

Synthesis of few-layered graphene by ion implantation of carbon in nickel thin films

The synthesis of few-layered graphene is performed by ion implantation of carbon species in thin nickel films, followed by high temperature annealing and quenching. Although ion implantation enables a precise control of the carbon content and of the uniformity of the in-plane carbon concentration in the Ni films before annealing, we observe thickness non-uniformities in the synthesized graphene layers after high temperature annealing. These non-uniformities are probably induced by the heterogeneous distribution/topography of the graphene nucleation sites on the Ni surface. Taken altogether, our results indicate that the number of graphene layers on top of Ni films is controlled by the nucleation process on the Ni surface rather than by the carbon content in the Ni film.     

Determination of glycated and acetylated hemoglobins in cord blood by time-of-flight mass spectrometry

The characterization of cord blood hemoglobin at the molecular level is a daunting challenge because hemoglobin F (HbF) and hemoglobin A (HbA) coexist in neonatal blood. We developed and validated a method using electrospray time-of-flight mass spectrometry (ES-TOF-MS) that measures, in a single analysis, relative levels of glycated and acetylated hemoglobin and allows the calculation of relative proportions of HbA, HbF(0), and HbF(1) in cord blood. Specific sections of acquired spectra were deconvoluted using a maximum entropy-based approach to true mass scale spectra. Mass precisions were less than 3 ppm with similar accuracies. Intra-interday precisions for α- and γ-chain glycation levels were 2.10%/3.72% and 2.75%/6.79%, respectively. The linearity of the α-chain glycation response was excellent (r(2) = 0.9990). We performed sample analysis on 39 cord blood specimens and found that the glycated α- and γ-chain levels were 2.27 ± 0.21% and 2.38 ± 0.29%, respectively, while the acetylated (G)γ and (A)γ-chain levels were 8.48 ± 0.53% and 7.14 ± 0.74%, respectively. We observed three types of HbF distinguishable by the intensities of γ-chain variants. Two-thirds of cord blood specimens were classified as HbF(I) with an intensity ratio (G)γ/(A)γ of 1.90 ± 0.12. For HbF(II) type (10/39 neonates), the intensity ratio of (G)γ/(A)γ was 3.71 ± 0.28. For three neonates with HbF(III), no (A)γ-chain was detected.     

In Vitro Rescue of the Bile Acid Transport Function of ABCB11 Variants by CFTR Potentiators

ABCB11 is responsible for biliary bile acid secretion at the canalicular membrane of hepatocytes. Variations in the ABCB11 gene cause a spectrum of rare liver diseases. The most severe form is progressive familial intrahepatic cholestasis type 2 (PFIC2). Current medical treatments have limited efficacy. Here, we report the in vitro study of Abcb11 missense variants identified in PFIC2 patients and their functional rescue using cystic fibrosis transmembrane conductance regulator potentiators. Three ABCB11 disease-causing variations identified in PFIC2 patients (i.e., A257V, T463I and G562D) were reproduced in a plasmid encoding an Abcb11-green fluorescent protein. After transfection, the expression and localization of the variants were studied in HepG2 cells. Taurocholate transport activity and the effect of potentiators were studied in Madin–Darby canine kidney (MDCK) clones coexpressing Abcb11 and the sodium taurocholate cotransporting polypeptide (Ntcp/Slc10A1). As predicted using three-dimensional structure analysis, the three variants were expressed at the canalicular membrane but showed a defective function. Ivacaftor, GLP1837, SBC040 and SBC219 potentiators increased the bile acid transport of A257V and T463I and to a lesser extent, of G562D Abcb11 missense variants. In addition, a synergic effect was observed when ivacaftor was combined with SBC040 or SBC219. Such potentiators could represent new pharmacological approaches for improving the condition of patients with ABCB11 deficiency due to missense variations affecting the function of the transporter.