Electron transfer by domain movement in cytochrome bc1

The cytochrome bc1 is one of the three major respiratory enzyme complexes residing in the inner mitochondrial membrane. Cytochrome bc1 transfers electrons from ubiquinol to cytochrome c and uses the energy thus released to form an electrochemical gradient across the inner membrane. Our X-ray crystal structures of the complex from chicken, cow and rabbit in both the presence and absence of inhibitors of quinone oxidation, reveal two different locations for the extrinsic domain of one component of the enzyme, an iron-sulphur protein. One location is close enough to the supposed quinol oxidation site to allow reduction of the Fe-S protein by ubiquinol. The other site is close enough to cytochrome c1 to allow oxidation of the Fe-S protein by the cytochrome. As neither location will allow both reactions to proceed at a suitable rate, the reaction mechanism must involve movement of the extrinsic domain of the Fe-S component in order to shuttle electrons from ubiquinol to cytochrome c1. Such a mechanism has not previously been observed in redox protein complexes.     

Nicalon-fibre-reinforced silicon-carbide composites via polymer solution infiltration and chemical vapour infiltration

A new, faster process was developed for the fabrication of Nicalon-fibre-reinforced SiC composites by combining polymer solution infiltration (PSI) and chemical vapour infiltration (CVI). The process led to the near-net-shape fabrication of fibre-reinforced ceramic-matrix composites and reduced infiltration time. Typical flexural strength and fracture toughness of these composites were 296 MPa and 10.9 MPa m^1/2 at room temperature (RT) and 252 MPa and 9.6 MPa m^1/2 at 1000 °C, respectively. The composites exhibited load-carrying capability after crack initiation.     

Acquisition of the mental state verb know by 2- to 5-year-old children

The production of the cognitive internal state word know by four 2- to 5-year-old children and their parents was examined. The levels of meaning of cognitive words can be categorized hierarchically along the dimensions of conceptual difficulty and abstractness (see Booth & Hall, 1995). The present study found that children and their parents expressed low levels of meaning less frequently, whereas they expressed high levels of meaning more frequently as a function of age. The children's use of know was also correlated positively with (1) their number of different words produced suggesting that cognitive words are related to more general semantic processes, and (2) with parental use of those same cognitive words suggesting that parental linguistic input may be an important mechanism in cognitive word acquisition. Finally, young children tended to use know more to refer to themselves than to refer to others, whereas their parents tended to use know equally to refer to self and others. The importance of cognitive words in a theory of language acquisition is discussed.     

Analysis of color symbology from the perspective of cultural semiotics focused on Korean costume colors according to the cultural changes

The purpose of this study was to analyze the symbolization of colors as cultural codes, based on costume colors. In order to study the significance of colors in cultural changes, we carried out a quantitative analysis and interpreted it from the perspective of cultural semiotics. The range of this study was focused on Korean costume colors, over diverse diachronic stages of Korean culture. For this study 1535 color samples were collected, measured with a spectrophotometer, and analyzed quantitatively according their diachronic stages of origin. As a result, red, blue, and yellow were found to be the most frequently used colors during the Chosun Dynasty, a period based on Confucianism. These colors acted as cultural codes with cultural significance. During the Modern times pink, light blue, and black increased in frequency and represented the reception of western culture, the changing sex role of women in society, and utilitarianism. In these days, neutral colors and grayish tones of all colors are the most significant colors of high frequency. The use of such colors is closely related to industrialization, mechanization, functionalism, and the changes of women's sex roles in the societies. They are used as cultural codes, especially to emphasize a rational and masculine image rather than a feminine image. © 2006 Wiley Periodicals, Inc. Col Res Appl, 32, 71–79, 2007; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/col.20290     

Analysis of the Sensitivity of Decision Analysis Results to Errors and Simplifications in Problem Structure: Application to Lake Erie Ecosystem Management

In practical decision analyses, the ldquocurse of dimensionalityrdquo compels one to make simplifying assumptions that can introduce errors into estimates of various indexes that interest decision-makers. These indexes include the expected performance of optimal and suboptimal strategies, the benefit of explicitly considering uncertainty, and the benefit of additional information. This paper quantifies the effects on these indexes of simplifying assumptions, including discretization of the decision space, omission of some decision variables and uncertainties from the decision tree, and disregarding of risk aversion. To reduce errors arising from discretization of the decision space, we use a multidimensional cubic spline to interpolate the performance of alternatives between a few simulated points. A case study analyzes decisions concerning phosphorus loading, fisheries management, and lower trophic research projects in Lake Erie under multiple criteria and ecological uncertainties. Results show that spline-based solutions often yield potentially superior decisions from those based on discretized decision spaces, but that omitting important uncertainties makes more of a difference in this case study's decisions and indexes than simplifying the decision space. On the other hand, incorrect multicriteria weights affect the case study's outcomes more than incorrect probabilities.     

Image flow segmentation and estimation using displaced spatial gradient

There are two main causes of inaccuracies in estimating image flows using gradient based techniques. One is the erroneous measurement of gradients in brightness and the other is the blurring of motion boundaries which is caused by the smoothness constraint. In the Letter, the gradient measurement error of conventional methods is analysed and a new technique based on this analysis proposed.<>