Comprehensive Analysis of hsa-miR-654-5p’s Tumor-Suppressing Functions

The pivotal roles of miRNAs in carcinogenesis, metastasis, and prognosis have been demonstrated recently in various cancers. This study intended to investigate the specific roles of hsa-miR-654-5p in lung cancer, which is, in general, rarely discussed. A series of closed-loop bioinformatic functional analyses were integrated with in vitro experimental validation to explore the overall biological functions and pan-cancer regulation pattern of miR-654-5p. We found that miR-654-5p abundance was significantly elevated in LUAD tissues and correlated with patients’ survival. A total of 275 potential targets of miR-654-5p were then identified and the miR-654-5p-RNF8 regulation axis was validated in vitro as a proof of concept. Furthermore, we revealed the tumor-suppressing roles of miR-654-5p and demonstrated that miR-654-5p inhibited the lung cancer cell epithelial-mesenchymal transition (EMT) process, cell proliferation, and migration using target-based, abundance-based, and ssGSEA-based bioinformatic methods and in vitro validation. Following the construction of a protein–protein interaction network, 11 highly interconnected hub genes were identified and a five-genes risk scoring model was developed to assess their potential prognostic ability. Our study does not only provide a basic miRNA-mRNA-phenotypes reference map for understanding the function of miR-654-5p in different cancers but also reveals the tumor-suppressing roles and prognostic values of miR-654-5p.     

用于星上图像压缩的JPEG2000算法的改进

本文修改了 JPEG2000 算法,通过改变编码顺序节约了大量的计算,提高了运行速度.用此算法对空间太阳望远镜的目标图像进行了实验,结果表明在相同压缩比的情况下,修改后的算法对图像质量没有明显的影响,在运行时间和压缩质量方面都能够满足空间太阳望远镜的压缩任务要求.     

Molecular Identification of the G-Protein Genes and Their Expression Profiles in Response to Nitrogen Deprivation in Brassica napus

Heterotrimeric guanine nucleotide binding protein (G-protein) consisting of Gα, Gβ, and Gγ subunits is one of the key signal transducers in plants. Recent studies indicated that G-protein has been proposed as an important mediator of nitrogen responses in rice, wheat, and Arabidopsis. However, little is known about these G-proteins in Brassica napus (B. napus), except for three identified G-proteins, BnGA1, BnGB1, and BnGG2. Therefore, the aim of the present study is to characterize the members of the G-protein gene family in allotetraploid B. napus and to analyze their expression profiles in response to nitrogen deprivation. In total, 21 G-protein family members were identified in B. napus, encoding two Gα, six Gβ, and 13 Gγ. Sequence and phylogenetic analyses showed that although genome-wide triploid events increased the number of genes encoding Gα, Gβ, and Gγ subunits, the gene structure and protein properties of the genes encoding each G-protein subunit were extremely conserved. Collinearity analysis showed that most G-protein genes in B. napus had syntenic relationships with G-protein members of Arabidopsis, Brassica rape (B. rapa), and Brassica oleracea (B. oleracea). Expression profile analysis indicated that Gα and C-type Gγ genes (except BnGG10 and BnGG12 were highly expressed in flower and ovule) were barely expressed in most organs, whereas most Gβ and A-type Gγ genes tended to be highly expressed in most organs. G-protein genes also showed various expression patterns in response to nitrogen-deficient conditions. Under nitrogen deficiency, Gα and five C-type Gγ genes were upregulated initially in roots, while in leaves, Gα was downregulated initially and five C-type Gγ genes were highly expressed in different times. These results provide a complex genetic dissection of G-protein genes in B. napus, and insight into the biological functions of G-protein genes in response to nitrogen deficiency.     

YJG4017型循环水泵节能改造探讨

主要介绍宜宾发电公司YJG4017型循环水泵节能技术改造方案及改造后的运行节能效果分析。     

VSP可重复性炮井高能高频炸药震源的试验

较理想的 VSP 炸药震源和激发条件应当达到如下的要求:1在较好的激发岩性和激发井深中激发,能准确地重复多次激发出频带较宽、高频成分丰富的有效波;2能在同一炮井、同一井深进行成百上千次的重复激发,仍然保持良好如初的激发条件。为此选用一种高速、高能量的小成型炸药,放置下有钢套管的、注满水的炮井中,在最佳的井深位置激发,能够达到上述要求。试验结果表明,这种炮井可重复激发千百次,所得记录的频谱和初至时间的重复性良好,频谱的主频可达40Hz,而药量只需几十克。     

多层住宅顶层改善措施

顶层住宅由于其用水、防水、隔热，上下楼不便等缺陷，不受居民的欢迎，在分配及销售上均存在着许多问题。本文对改善顶层住房的舒适性方面，提出了多种综合性措施，将顶层的不利因素转化为有利因素，具有一定的推广价值。     

IP网上的监视器--NP2000网络流量探测系统介绍

介绍发ＮＰ２０００网络流量探测管理系统的整体结构及应用模式。本系统实现了在Ｗｅｂ的基础上完成子网流量监视、栓错告警、包捕获、协议分析和网络规划等强大而灵活的网络管理功能。