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181.
We studied the molecular basis of protein C deficiency in a family with a history of thromboembolic disease. An approximately 50% reduction in anticoagulant activity despite normal levels of protein C amidolytic activity and antigen was detected in plasma from the proband. All the exons and intron/exon junctions of the protein C gene were studied using a strategy that combined polymerase chain reaction amplification with DNA sequencing of the amplified fragments. We identified a C-to-A change at nucleotide number 1387 of the protein C gene in the proband and his mother, and this mutant was designated protein C Osaka 10. The C-to-A change resulted in the substitution of Ser for Arg at position -1, which is the processing protease cleavage site. The mutant protein C was partially purified from plasma of the patient's mother using barium adsorption followed by ion-exchange column chromatography. It eluted at the same sodium chloride concentration as normal protein C, and thus gamma-carboxylation of the mutant protein appeared to be normal. The apparent molecular weight of this mutant protein C was the same as that of the normal protein on immunoblotting. Amino-terminal sequence analysis showed that the light chain of the mutant protein C had an additional Ser at position-1. Thus, the loss of anticoagulant activity of protein C Osaka 10 can be explained by alteration of the conformation of the Gla domain by the additional Ser in the mutant molecule. 相似文献
182.
PURPOSE: To demonstrate a superselective intraarterial chemotherapy as a therapeutic alternative in the treatment of previously treated recurrent lymph node metastases in breast cancer. METHODS: 14 patients with recurrent lymph node metastases in cases of breast cancer were presented to be treated by intraarterial chemotherapy of 25 mg mitoxantrone/m2 over a period of 24 hours. In two patients with superclavicular lymph node involvement an intraarterial therapy could not be carried out because of a vascular connection to the anterior spinal artery. Involved lymph stations could be reached in superselective technique by side branches of the subclavian artery. Heparin coverage was given intravenously. Every patient had had surgery, radiation, systemic chemo- and hormonal therapy before and was now graded as inoperable. Therapy indication was given by local tumour-induced symptoms. RESULTS: In the 12 treated cases complete remission was seen in three, partial remission in 4, a steady state in two and a progressive disease in three. There were no complications or severe side effects. CONCLUSION: Intraarterial chemotherapy is an effective and well tolerated treatment in recurrent lymph node metastases in cases of breast cancer even if conventional therapies can no longer be used. 相似文献
183.
Several distributed power control algorithms that can achieve carrier-to-interference ratio (CIR) balancing with probability one have been proposed previously for cellular mobile systems. In these algorithms, only local information is used to adjust transmitting power. However, a normalization procedure is required in each iteration to determine transmitting power and, thus, these algorithms are not fully distributed. In this paper, we present a distributed power control algorithm which does not need the normalization procedure. We show that the proposed algorithm can achieve CIR balancing with probability one. Moreover, numerical results reveal our proposed scheme performs better than the algorithm presented in Grandhi et al. [1994]. The excellent performance and the fully distributed property make our proposed algorithm a good choice for cellular mobile systems 相似文献
184.
Yeong Joo Lee 《International Journal of Fracture》1992,57(1):25-46
The two-dimensional problem of an edge crack in a half space or plate is considered. The body is loaded by a suddenly applied, spatially uniform normal velocity imposed on the plane boundary of the body on one side of the edge crack. Otherwise, the boundary of the body, including the crack faces, is traction free. Both cases of an initially sharp crack tip and a narrow notch with small but nonzero notch root radius are considered. The material is modeled as elastic viscoplastic, including strain hardening, rate sensitivity and thermal softening. The applied loading produces predominantly mode II loading in the crack tip region. Under these conditions it is possible to nucleate an adiabatic shear band at the crack tip as a precursor to a mode II fracture. On the other hand, because of the rate sensitivity of the material and the high rate of loading, it may be possible under certain conditions to generate tensile stresses in the crack tip region sufficiently large to nucleate brittle tensile fracture. The problem is solved numerically by means of the finite element method in order to investigate the competition between these two possible fracture initiation mechanisms. The magnitude of the impact velocity imposed on the edge of the plate and the notch tip acuity have an effect on processes near the crack tip. For given material, the inception of crack growth is determined by the competition between a stress-based brittle fracture condition, associated with rate sensitivity and strain hardening, and a strain based criterion, associated with high strain rate and thermal softening. 相似文献
185.
A quartz microbalance technique has been used to study the growth of4He and H2 adsorbed on sodium. In contrast to cesium, adsorption of4He and H2 do occur on a sodium surface. 相似文献
186.
Yi-Min Wang Hua Lee Dipali V. Apte 《International journal of imaging systems and technology》1992,4(3):201-206
The matrix pencil (MP) method, based on the singular value decomposition (SVD), is applied to quantitative NMR spectroscopy. Its relationship to other SVD-based methods is also presented. Simulations and applications are given to demonstrate the capability of superior performance.©1993 John Wiley & Sons Inc 相似文献
187.
The effect of DC flux on the core loss is examined for the practical range of power and frequency. Relevant core loss equations are derived and applied to an optimization algorithm to determine the minimum core loss at a given ratio of s (DC flux density to AC peak flux density). It has been found that the curves of hysteresis loss density versus the ratio of s exhibit a peak at a critical ratio. Below or above this critical ratio, the loss density decreases drastically. On the other hand, the curves of eddy-current loss density versus the ratio of s exhibits a minimum point at a critical ratio. Below or above this critical ratio, the loss density increases gradually 相似文献
188.
Y. C. Lee Ph.D. Nagesh Basavanhally Ph.D. 《JOM Journal of the Minerals, Metals and Materials Society》1994,46(6):46-50
In addition to providing electrical connections, solder is useful in the formation of passive, precision alignments for optoelectronic packaging. Different designs have demonstrated micrometer-level alignment accuracies, and the aligned structures are becoming more and more complex. Solder engineering has been successfully introduced into the field of optoelectronics, and it is expected to be applied to many other areas demanding low-cost, precision alignments. 相似文献
189.
190.