Genome-Wide Identification of TaSAUR Gene Family Members in Hexaploid Wheat and Functional Characterization of TaSAUR66-5B in Improving Nitrogen Use Efficiency

Excessive input of nitrogen fertilizer not only causes a great waste of resources but brings about a series of ecological and environmental problems. Although Small Auxin Up-regulated RNAs (SAURs) participate in diverse biological processes, the function of SAURs in the nitrogen starvation response has not been well-studied. Here, we identified 308 TaSAURs in wheat and divided them into 10 subfamilies. The promoter regions of most TaSAURs contain hormone responsive elements, and their expression levels change under the treatment of different hormones, such as IAA, MeJA, and ABA. Interestingly, overexpression of one of the TaSAUR family members, a nitrogen starvation responsive gene, TaSAUR66-5B, can promote the growth of Arabidopsis and wheat roots. In addition, overexpression of TaSAUR66-5B in Arabidopsis up-regulates the expression levels of auxin biosynthesis related genes, suggesting that overexpression TaSAUR66-5B may promote root growth by increasing the biosynthesis of auxin. Furthermore, overexpression of TaSAUR66-5B in wheat can increase the biomass and grain yields of transgenic plants, as well as the nitrogen concentration and accumulation of both shoots and grains, especially under low nitrogen conditions. This study provides important genomic information of the TaSAUR gene family and lays a foundation for elucidating the functions of TaSAURs in improving nitrogen utilization efficiency in wheat.     

CALMODULIN1 and WRKY53 Function in Plant Defense by Negatively Regulating the Jasmonic Acid Biosynthesis Pathway in Arabidopsis

Jasmonic acid (JA) is an important hormone that functions in plant defense. cam1 and wrky53 mutants were more resistant to Spodoptera littoralis than in the wild-type (WT) Arabidopsis group. In addition, JA concentration in cam1 and wrky53 mutants was higher compared with the WT group. To explore how these two proteins affect the resistance of Arabidopsis plants, we used a yeast two-hybrid assay, firefly luciferase complementation imaging assay and in vitro pull-down assay confirming that calmodulin 1 (CAM1) interacted with WRKY53. However, these two proteins separate when calcium concentration increases in Arabidopsis leaf cells. Then, electrophoretic mobility shift assay and luciferase activation assay were used to verify that WRKY53 could bind to lipoxygenases 3 (LOX3) and lipoxygenases 4 (LOX4) gene promoters and negatively regulate gene expression. This study reveals that CAM1 and WRKY53 negatively regulate plant resistance to herbivory by regulating the JA biosynthesis pathway via the dissociation of CAM1-WRKY53, then the released WRKY53 binds to the LOXs promoters to negatively regulate LOXs gene expression. This study reveals WRKY53′s mechanism in insect resistance, a new light on the function of WRKY53.     

SiN钝化前的NF3等离子体处理对AlGaN/GaN HEMT性能的影响

研究了SiN钝化前利用感应耦合等离子体(ICP)对AlGaN/GaN HEMT表面进行NF3等离子体处理对器件性能的影响.结果表明,运用低能量的NF3等离子体处理钝化前的AlGaN/GaN HEMT表面能有效抑制器件电流崩塌,而器件直流及微波小信号特性则未受影响.微波功率测试表明,经过6min NF3等离子体处理的AIGaN/GaN HEMT在2GHz,30V工作电压下达到6.15W/mm的输出功率密度,而未经过处理的器件只达到1.82W/mm的输出功率密度.     

Modelling corn production in China using AVHRR‐based vegetation health indices

Weather‐related crop losses have always been a concern for farmers, governments, traders and policy makers for the purpose of balanced food supplies, demands, trade, and distribution of aid to nations in need. Therefore, early crop loss assessment in response to weather fluctuations is an important issue. This paper discusses the utility of Advanced Very High Resolution Radiometer (AVHRR)‐based vegetation health indices as a proxy for modelling corn yield and for early warning of drought‐related losses of agricultural production in China. The indices were tested in Jilin province on corn yield during 1982–2001 using correlation and regression analysis. A strong correlation between corn yield and the vegetation health indices were found during the critical period of corn growth, which starts 2–3 weeks before and 2–3 weeks after corn tassel. Following the results of correlation analysis, several regression equations were constructed where vegetation health indices were used as independent variables. The estimates of corn yield can be carried out well in advance of harvest and the errors of the estimates are 7–10%. The errors become smaller when the estimations are related to losses in corn yield due to drought.     

Essential oil composition and antimicrobial activity of Sphallerocarpus gracilis seeds against selected food-related bacteria

Sphallerocarpus gracilis from China is a little-investigated edible and medicinal plant. In the present study, the essential oil composition from S. gracilis seeds was investigated by GC and GC–MS. A total of 34 compounds representing 94.69% of the essential oil were tentatively identified. The main constituents were p-cymene (17.42%), γ-terpinene (25.58%) and α-asarone (33.12%). The antimicrobial activity, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of the essential oil from S. gracilis seeds were evaluated against eight Gram-positive bacteria (Listeria monocytogenes, Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus luteus, Bacillus megaterium, Bacillus cereus, Bacillus coagulans and Bacillus subtilis), four Gram-negative bacteria (Salmonella enteritidis, Salmonella typhimurium, Klebsiella pneumoniae and Escherichia coli) and one fungus (Aspergillus niger). Results revealed that the essential oil from S. gracilis seeds exhibited significant in vitro antimicrobial property. Among all the tested microorganisms, the essential oil showed the strongest inhibitory effect against K. pneumoniae, whereas no inhibitory effect was found against L. monocytogenes and A. niger. Additionally, scanning electron microscopy (SEM) was used to observe morphological changes of bacteria treated with the essential oil from S. gracilis seeds. SEM observations confirmed the physical damage and considerable morphological alteration to the tested microorganisms treated with the essential oil. The data of this study suggests that the essential oil from S. gracilis seeds has great potential for application as a natural antimicrobial agent to preserve food.     

关于冶金机械设备几种事故驱动方式的比较

比较了冶金机械设备在停电故障下几种事故驱动方式,重点介绍了新兴的EPS(Emergency Power Supply)事故驱动电源,并倾向于采用EPS作为冶金设备的主要事故驱动。     

平地机耐磨衬垫的改进设计

<正>为降低牵引架与回转圈的摩擦,徐工生产的平地机都装有耐磨衬垫。图1为平地机回转圈、牵引定位用AB胶粘在牵引架上,在回转圈回转过程中起到保护牵引架和回转圈的作用。平地机工作过程中,由于回转圈与牵引架相对运动时产生的冲击,     

机械租赁经营研究

以某国有施工企业为研究对象,就其机械租赁战略进行了分析研究并从市场营销理论和市场发展战略2个角度,探讨了我国工程机械行业租赁经营的发展状况和未来发展方向。