A Comprehensive Identification and Function Analysis of Serine/Arginine-Rich (SR) Proteins in Cotton (Gossypium spp.)

As one of the most important factors in alternative splicing (AS) events, serine/arginine-rich (SR) proteins not only participate in the growth and development of plants but also play pivotal roles in abiotic stresses. However, the research about SR proteins in cotton is still lacking. In this study, we performed an extensive comparative analysis of SR proteins and determined their phylogeny in the plant lineage. A total of 169 SR family members were identified from four Gossypium species, and these genes could be divided into eight distinct subfamilies. The domain, motif distribution and gene structure of cotton SR proteins are conserved within each subfamily. The expansion of SR genes is mainly contributed by WGD and allopolyploidization events in cotton. The selection pressure analysis showed that all the paralogous gene pairs were under purifying selection pressure. Many cis-elements responding to abiotic stress and phytohormones were identified in the upstream sequences of the GhSR genes. Expression profiling suggested that some GhSR genes may involve in the pathways of plant resistance to abiotic stresses. The WGCNA analysis showed that GhSCL-8 co-expressed with many abiotic responding related genes in a salt-responding network. The Y2H assays showed that GhSCL-8 could interact with GhSRs in other subfamilies. The subcellular location analysis showed that GhSCL-8 is expressed in the nucleus. The further VIGS assays showed that the silencing of GhSCL-8 could decrease salt tolerance in cotton. These results expand our knowledge of the evolution of the SR gene family in plants, and they will also contribute to the elucidation of the biological functions of SR genes in the future.     

TaNBR1, a Novel Wheat NBR1-like Domain Gene Negatively Regulates Drought Stress Tolerance in Transgenic Arabidopsis

Drought stress is an important factor that severely affects crop yield and quality. Autophagy has a crucial role in the responses to abiotic stresses. In this study, we explore TaNBR1 in response to drought stress. Expression of the TaNBR1 gene was strongly induced by NaCl, PEG, and abscisic acid treatments. The TaNBR1 protein is localized in the Golgi apparatus and autophagosome. Transgenic Arabidopsis plants overexpressing TaNBR1 exhibited reduced drought tolerance. When subjected to drought stress, compared to the wild-type (WT) lines, the transgenic overexpressing TaNBR1 plants had a lower seed germination rate, relative water content, proline content, and reduced accumulation of antioxidant enzymes, i.e., superoxide dismutase, peroxidase, and catalase, as well as higher chlorophyll losses, malondialdehyde contents, and water loss. The transgenic plants overexpressing TaNBR1 produced much shorter roots in response to mannitol stress, in comparison to the WT plants, and they exhibited greater sensitivity to abscisic acid treatment. The expression levels of the genes related to stress in the transgenic plants were affected in response to drought stress. Our results indicate that TaNBR1 negatively regulates drought stress responses by affecting the expression of stress-related genes in Arabidopsis.     

深度双重注意力的生成与判别联合学习的行人重识别

在行人重识别任务中存在数据集标注难度大,样本量少,特征提取后细节特征缺失等问题。针对以上问题提出深度双重注意力的生成与判别联合学习的行人重识别。首先,构建联合学习框架,将判别模块嵌入生成模块,实现图像生成和判别端到端的训练,及时将生成图像反馈给判别模块,同时优化生成模块与判别模块。其次,通过相邻的通道注意力模块间连接和相邻空间注意力模块间连接,融合所有通道特征和空间特征,构建深度双重注意力模块,将其嵌入教师模型,使模型能更好地提取行人细节身份特征,提高模型识别能力。实验结果表明,该算法在Market-1501和DukeMTMC-ReID数据集上具有较好的鲁棒性、判别性。     

A Novel Human Antibody,HF, against HER2/erb-B2 Obtained by a Computer-Aided Antibody Design Method

Fully human antibodies have minimal immunogenicity and safety profiles. At present, most potential antibody drugs in clinical trials are humanized or fully human. Human antibodies are mostly generated using the phage display method (in vitro) or by transgenic mice (in vivo); other methods include B lymphocyte immortalization, human–human hybridoma, and single-cell polymerase chain reaction. Here, we describe a structure-based computer-aided de novo design technology for human antibody generation. Based on the complex structure of human epidermal growth factor receptor 2 (HER2)/Herceptin, we first designed six short peptides targeting the potential epitope of HER2 recognized by Herceptin. Next, these peptides were set as complementarity determining regions in a suitable immunoglobulin frame, giving birth to a novel anti-HER2 antibody named “HF,” which possessed higher affinity and more effective anti-tumor activity than Herceptin. Our work offers a useful tool for the quick design and selection of novel human antibodies for basic mechanical research as well as for imaging and clinical applications in immune-related diseases, such as cancer and infectious diseases.     

腈纶生产装置危险化学品储存于安全管理

根据危险化学品易燃、易爆、有毒的特点,提出安全储存和管理的管理思路,同时探讨了装卸入库的安全注意管理点.     

Electron beam induced structural evolution in Fe3O4SiO2 particles: A new route to obtain movable core structures

SiO₂ hollow spheres with movable Fe₃O₄ core were obtained by exposing the pre-synthesized Fe₃O₄SiO₂ particles (with an adsorption interlayer of ethylene glycol) under the irradiation of electron beam inside transmission electron micrograph (TEM). In the formation process, the evaporation of adsorbed ethylene glycol and the evolution of amorphous SiO₂ layer played important roles, and that should be attributed to the high temperature and trapped charges induced by the irradiation of electron beam. This work provided a new route to obtain particles with movable core structure and extended the applications of electron beam.     

Microstructure and Transport Properties in Tin Boned La<Subscript>0.7</Subscript>Sr<Subscript>0.3</Subscript>MnO<Subscript>3</Subscript> Composites

A novel boned perovskite manganese oxide magnetoresistant material was prepared using La_0.7Sr_0.3MnO₃ (LSMO) as the precursor powders and metal tin (Sn) as the binder. The microstructure and phase characteristics, low-field transport properties were studied. Sn segregated at the grain boundaries of LSMO grains. The insulator–metal (I–M) transition and enhanced LFMR are only observed with a low content of Sn, due to grain boundary disorder and spin polarized tunneling between grain boundaries. The Sn addition induced resistivity decreasing dramatically. In the high temperature PM region, the resistivities for samples with low Sn content follow the adiabatic small-polaron-hopping model.     

巴西、阿根廷、古巴三国照明产品质量监管体系及其对我国的启示

阐述了巴西、阿根廷、古巴节能产品质量监管的基本情况,系统分析对比了巴西、阿根廷、古巴以及欧美等国的照明产品质量监管体系的特点和有益经验。在此基础上,提出了建立健全我国照明产品质量监管体系的若干建议。     

冰箱门封导热性能及影响因素的CAE分析

由于冰箱门封对于节能非常重要,本文利用CAE仿真技术,采用二维平面模型对多款冰箱门封进行仿真研究,为冰箱门封和冰箱的性能设计和优化提供理论依据。通过对典型冰箱门封导热性能的分析,找出影响门封导热性能的因素,同时,以某型号冰箱为例,研究门封对冰箱漏冷量的影响。仿真结果表明,门封结构及磁条是门封导热性能的重要因素;门封等效导热系数约为0.05W/(m·K);冰箱门封漏冷占冰箱总漏冷约8%;门封高度从10mm减小为7mm时,冰箱门封漏冷比率减小约2.3%。     

宽水压进水系统及其洗衣机的研究应用

本文通过对现有进水阀结构和控制方式的改变,实现在较低水压下的较大流量进水,满足低水压情况下全自动洗衣机的进水要求,同时兼顾正常水压情况下的洗衣机使用,使之更适用于广大农村市场。同时,避免活塞帽在隔膜板活动空间内抖动产生的噪声,给消费者带来不便。