Application potential of bone marrow mesenchymal stem cell (BMSCs) based tissue-engineering for spinal cord defect repair in rat fetuses with spina bifida aperta

Spina bifida aperta are complex congenital malformations resulting from failure of fusion in the spinal neural tube during embryogenesis. Despite surgical repair of the defect, most patients who survive with spina bifida aperta have a multiple system handicap due to neuron deficiency of the defective spinal cord. Tissue engineering has emerged as a novel treatment for replacement of lost tissue. This study evaluated the prenatal surgical approach of transplanting a chitosan–gelatin scaffold seeded with bone marrow mesenchymal stem cells (BMSCs) in the healing the defective spinal cord of rat fetuses with retinoic acid induced spina bifida aperta. Scaffold characterisation revealed the porous structure, organic and amorphous content. This biomaterial promoted the adhesion, spreading and in vitro viability of the BMSCs. After transplantation of the scaffold combined with BMSCs, the defective region of spinal cord in rat fetuses with spina bifida aperta at E20 decreased obviously under stereomicroscopy, and the skin defect almost closed in many fetuses. The transplanted BMSCs in chitosan–gelatin scaffold survived, grew and expressed markers of neural stem cells and neurons in the defective spinal cord. In addition, the biomaterial presented high biocompatibility and slow biodegradation in vivo. In conclusion, prenatal transplantation of the scaffold combined with BMSCs could treat spinal cord defect in fetuses with spina bifida aperta by the regeneration of neurons and repairmen of defective region.     

马钢2500m~3高炉炉渣的冶金性能

在目前的原燃料条件下,为保证马钢2500m3高炉炉渣具有良好的流动性和较高的脱硫能力,炉渣的CaO／SiO2应控制在1．05至1．15之间,炉渣温度不低于1450℃．     

Tissue dynamics spectroscopy for three-dimensional tissue-based drug screening

Tissue dynamics spectroscopy combines dynamic light scattering with short-coherence digital holography to capture intracellular motion inside multicellular tumor spheroid tissue models. The cellular mechanical activity becomes an endogenous imaging contrast agent for motility contrast imaging. Fluctuation spectroscopy is performed on dynamic speckle from the proliferating shell and hypoxic core to generate drug-response spectrograms that are frequency versus time representations of the changes in spectral content induced by an applied compound or an environmental perturbation. A combination of 28 reference compounds and conditions applied to rat osteogenic UMR-106 spheroids generated spectrograms that were crosscorrelated in a similarity matrix used for unsupervised hierarchical clustering of similar compound responses. This work establishes the feasibility of tissue dynamics spectroscopy for three-dimensional tissue-based phenotypic profiling of drug response as a fully endogenous probe of the response of tissue to reference compounds.     

Piecewise Pseudo-Maximum Likelihood Estimation for Risk Aversion Case in First-Price Sealed-Bid Auction

In the literature, more and more clues show that the bidders indeed tend to risk averse. But as a good structure estimation procedure in first-price sealed-bid auctions, the traditional PPMLE (Piecewise Pseudo-Maximum Likelihood Estimation) approach is only applicable to the risk neutrality case. This study generalizes this approach to the risk aversion case, and obtains a simple estimation procedure for the Pareto family of private costs. In the procedure, the estimation order of distribution parameters of private costs is not important, which is different from the traditional PPMLE method. Though there is no unique estimator for θ ₁ and γ, some guidance is given for real-world applications on the basis of our Monte Carlo simulation experiments. What’s more, this procedure can be reduced further, if one is only interested in estimating the private costs. Extensive simulation experimental results indicate that our approach outperforms or matches at least the traditional one in the case of risk neutrality, and is applicable to the risk aversion case.     

Shrinkage estimation in general linear models

We propose a James-Stein-type shrinkage estimator for the parameter vector in a general linear model when it is suspected that some of the parameters may be restricted to a subspace. The James-Stein estimator is shown to demonstrate asymptotically superior risk performance relative to the conventional least squares estimator under quadratic loss. An extensive simulation study based on a multiple linear regression model and a logistic regression model further demonstrates the improved performance of this James-Stein estimator in finite samples. The application of this new estimator is illustrated using Ontario newborn infants data spanning four fiscal years.     

Prediction of rank deficiency in partition of unity-based methods with plane triangular or quadrilateral meshes

In the partition of unity (PU)-based methods, the global approximation is built by multiplying a partition of unity by local approximations. Within this framework, high-order approximations are achieved by directly adopting high-order polynomials as local approximations, and therefore nodes along sides or inside elements, which are usually adopted in the conventional finite element methods, are no more required. However, the PU-based approximation constructed in this way may suffer from rank deficiency due to the linear dependence of the global degrees of freedom. In this paper, the origin of the rank deficiency in the PU-based approximation space is first dissected at an element level, and then an approach to predict the rank deficiency for a mesh is proposed together with the principle of the increase of rank deficiency. Finally, examples are investigated to validate the present approach. The current work indicates such a fact that the rank deficiency is an unrelated issue to the nullity of the global matrix. It can be resolved in its own manner.     

Synthesis of Single-Crystalline Silicon Nitride Nanobelts Via Catalyst-Assisted Pyrolysis of a Polysilazane

Recently, one-dimensional nanostructures have attracted extensive attention since they are potentially important for both applications and fundamental research. In this paper, we report the synthesis of ultra-long single crystal Si₃N₄ nanobelts via catalyst-assisted pyrolysis of polymeric precursors. The obtained products contain both α- and β-Si₃N₄ nanobelts, which are 50–100 nm in thickness, 400–1000 nm in width, and a few hundreds of micrometers to several millimeters in length. Different from previous techniques for synthesizing one-dimensional structures, the current nanobelts are synthesized through confined crystallization of an amorphous phase. A solid–liquid–gas–solid reaction/crystallization growth mechanism is proposed. The formation of nanobelts instead of nanowires is attributed to the anisotropy growth at an earlier stage.     

库什塔依水电站工程建设质量管理

库什塔依水电站工程面临着工期紧、任务重、沥青混凝土冬季施工等问题,给现场的施工管理带来了挑战。在建设过程中严格执行中电投新疆公司管理制度,通过建立健全质量保证体系,完善质量保证措施,强化参建各方的质量责任意识,加强过程监控,实现了工程建设的质量目标。     

Inadequacy of Land Use and Impervious Area Fraction for Determining Urban Stormwater Quality

Urban stormwater quality is multifaceted and the use of a limited number of factors to represent catchment characteristics may not be adequate to explain the complexity of water quality response to a rainfall event or site-to-site differences in stormwater quality modelling. This paper presents the outcomes of a research study which investigated the adequacy of using land use and impervious area fraction only, to represent catchment characteristics in urban stormwater quality modelling. The research outcomes confirmed the inadequacy of the use of these two parameters alone to represent urban catchment characteristics in stormwater quality prediction. Urban form also needs to be taken into consideration as it was found have an important impact on stormwater quality by influencing pollutant generation, build-up and wash-off. Urban form refers to characteristics related to an urban development such as road layout, spatial distribution of urban areas and urban design features.