Principles in the Evolutionary Design of Digital Circuits—Part I

An evolutionary algorithm is used as an engine for discovering new designs of digital circuits, particularly arithmetic functions. These designs are often radically different from those produced by top-down, human, rule-based approaches. It is argued that by studying evolved designs of gradually increasing scale, one might be able to discern new, efficient, and generalizable principles of design. The ripple-carry adder principle is one such principle that can be inferred from evolved designs for one and two-bit adders. Novel evolved designs for three-bit binary multipliers are given that are 20% more efficient (in terms of number of two-input gates used) than the most efficient known conventional design.     

Organization and mobility of water in amorphous and crystalline trehalose

The disaccharide trehalose is accumulated by microorganisms, such as yeasts, and multicellular organisms, such as tardigrades, when conditions of extreme drought occur. In this way these organisms can withstand dehydration through the formation of an intracellular carbohydrate glass, which, with its high viscosity and hydrogen-bonding interactions, stabilizes and protects the integrity of complex biological structures and molecules. This property of trehalose can also be harnessed in the stabilization of liposomes, proteins and in the preservation of red blood cells, but the underlying mechanism of bioprotection is not yet fully understood. Here we use positron annihilation lifetime spectroscopy to probe the free volume of trehalose matrices; specifically, we develop a molecular picture of the organization and mobility of water in both amorphous and crystalline states. Whereas in amorphous matrices, water increases the average intermolecular hole size, in the crystalline dihydrate it is organized as a confined one-dimensional fluid in channels of fixed diameter that allow activated diffusion of water in and out of the crystallites. We present direct real-time evidence of water molecules unloading reversibly from these channels, thereby acting as both a sink and a source of water in low-moisture systems. We postulate that this behaviour may provide the overall stability required to keep organisms viable through dehydration conditions.     

Robustness and static-positional accuracy of the SteamVR 1.0 virtual reality tracking system

Virtual Reality - The use of low-cost immersive virtual reality systems is rapidly expanding. Several studies started to analyse the accuracy of virtual reality tracking systems, but they did not...     

Purification and properties of the chloroplastic form of biotin holocarboxylase synthetase from Arabidopsis thaliana overexpressed in Escherichia coli

Holocarboxylase synthetases (HCSs) are key enzymes in biotin utilisation in both prokaryotes and eukaryotes. In a previous study, we demonstrated that, in plants, HCS activity is localised in cytosol, chloroplasts and mitochondria. We also described the cloning and sequencing of a full-length cDNA encoding an Arabidopsis thaliana HCS isoform with a putative organelle-transit peptide. In the study reported here, this cDNA was used to construct an overproducing Escherichia coli strain. The recombinant enzyme was isolated using an efficient three-step purification procedure. Polyclonal antibodies raised against pure HCS were produced to elucidate the subcellular localisation of this protein. Immunodetection carried out by Western blotting of isolated pea leaf subcellular compartments specifically revealed a single polypeptide that was ascribed to the chloroplast compartment. Immunocytochemistry of thin-cut sections from tobacco leaves, transformed by the complete coding sequence of A. thaliana HCS cDNA via Agrobacterium tumefaciens, confirmed that the enzyme encoded by this cDNA is the chloroplastic isoform. Moreover, physicochemical, biochemical and kinetic properties of the pure recombinant HCS were determined. The native recombinant enzyme is a 37-kDa monomer. In contrast to the major part of HCS activity measured in leaf extracts, the recombinant chloroplastic enzyme did not require addition of Mg2+ to be fully active, but was substantially inhibited by EDTA. This suggested that the chloroplastic HCS may contain a tightly-bound divalent cation required for enzyme activity. The recombinant enzyme was able to biotinylate efficiently apo-biotin carboxyl carrier protein (BCCP) from E. coli and apo-methylcrotonoyl-CoA carboxylase (MCCase) from A. thaliana. Apparent Km values for the enzyme substrates D-biotin, ATP and apo-MCCase were found to be 130 nM, 4.4 microM and 32 microM, respectively. Steady-state kinetic analyses of the HCS-catalysed reaction were investigated with respect to reaction mechanism and inhibition by AMP, one of the end-products of the enzyme-catalysed reaction. Substrate interaction and product inhibition patterns indicated that ATP and D-biotin bind sequentially, in an ordered manner, to the enzyme and that ATP or D-biotin and apo-BCCP bind in ping-pong fashion.     

In situ X-ray diffraction of prototype sodium metal halide cells: Time and space electrochemical profiling

The feasibility of using energy dispersive X-ray diffraction to characterize full size battery cells is demonstrated by unprecedented in situ measurements of the electrochemical processes taking place inside high temperature sodium metal halide (Na/MCl₂, M = Ni and/or Fe) cells during charge/discharge cycling. Diffraction data provide phase information either via line scans across the 5 cm wide cells or via fixed location scans as a function of time. The data confirm the propagation of a well-defined chemical reaction front, as a function of charge/discharge time, beginning at the ceramic separator and proceeding inward. Measurement of the temporal evolution of the phase abundances yields mechanistic understanding and reaction rates as a function of charge/discharge state. In the case where M includes Fe, the data also clearly show the appearance of an intermediate phase, Na₆FeCl₈, during charging, thereby underscoring the power of this technique to reveal subtle mechanistic information. A number of additional detailed electrochemical kinetic effects are also discussed. This study shows that in situ high energy X-ray diffraction characterization of advanced battery cells in space and time is eminently feasible on a routine basis, and has great potential to advance the understanding of “buried” chemical processes.     

Bio-hydrogen production based on catalytic reforming of volatiles generated by cellulose pyrolysis: An integrated process for ZnO reduction and zinc nanostructures fabrication

The paper presents a process of cellulose thermal degradation with bio-hydrogen generation and zinc nanostructures synthesis. Production of zinc nanowires and zinc nanoflowers was performed by a novel processes based on cellulose pyrolysis, volatiles reforming and direct reduction of ZnO. The bio-hydrogen generated in situ promoted the ZnO reduction with Zn nanostructures formation by vapor-solid (VS) route. The cellulose and cellulose/ZnO samples were characterized by thermal analyses (TG/DTG/DTA) and the gases evolved were analyzed by FTIR spectroscopy (TG/FTIR). The hydrogen was detected by TPR (Temperature Programmed Reaction) tests. The results showed that in the presence of ZnO the cellulose thermal degradation produced larger amounts of H₂ when compared to pure cellulose. The process was also carried out in a tubular furnace with N₂ atmosphere, at temperatures up to 900 °C, and different heating rates. The nanostructures growth was catalyst-free, without pressure reduction, at temperatures lower than those required in the carbothermal reduction of ZnO with fossil carbon. The nanostructures were investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). The optical properties were investigated by photoluminescence (PL). One mechanism was presented in an attempt to explain the synthesis of zinc nanostructures that are crystalline, were obtained without significant re-oxidation and whose morphologies are dependent on the heating rates of the process. This route presents a potential use as an industrial process taking into account the simple operational conditions, the low costs of cellulose and the importance of bio-hydrogen and nanostructured zinc.     

NMR and quenched molecular dynamics studies of superpotent linear and cyclic alpha-melanotropins

Conformational searching, computer simulations, synthesis and NMR are used on a variety of alpha melanocyte-stimulating hormone (alpha-MSH) analogues to understand the physical characteristics required for biological potency. Peptides I (Ac-[Nle4,Asp5,D-Phe7,Lys10]alpha-MSH(4-10)-NH2), II (Ac-c[Nle4,Asp5,D-Phe7,Lys10]alpha-MSH(4-10)-NH2) and III (Ac-[Nle4,Asp5,D-Phe7,Dap10]alpha-MSH(4-10)-NH2 all show very similar conformational properties (backbone and side-chain torsional angles), and all display high biological potencies. The modeling results for these compounds are supported by the NMR data. Peptide IV (Ac-c[Nle4,Asp5,D-Phe7,Dap10]alpha-MSH(4-10)-NH2) appears to have a markedly different conformation and has decreased biological potency.     

Decision support tools for site-specific fertilizer recommendations and agricultural planning in selected countries in sub-Sahara Africa

Recommendations and decisions of crop management in sub-Saharan Africa (SSA) are often based on traditional field experimentation. This usually ignores the variability of production factors in space and time, and hence invalidates such decisions and recommendations outside of the experimental sites. Yet, the use of alternative or complementary decision support approaches such as crop modelling is limited. In this paper, we reviewed the state of the use of crop modelling in informing site specific fertilizer recommendations in some countries in SSA. Even though nitrogen fertilizer recommendations in most countries across Africa are blanket, the limited employment of models show that optimum nitrogen application should be differentiated according to soil types, management and climate. A number of studies reported on increased fertilizer use efficiency and reduced crop production risks with the use of decision support tools (DST). The review also showed that the gross limitation of the use of models as agricultural decision-making tools in SSA could be attributed to factors such as low capacity due to limited training opportunities, and the general lack of support from national governments for model development and application for policy formulation. Proposals identified to overcome these limitations include (1) introduction of the science of DST in the curricula at the tertiary level, (2) encouragement and support for the adoption of model use by governmental and non-governmental organizations as additional tools for decision making and (3) simplifying DSTs to facilitate their use by non-scientific audience to scale uptake and use for farm management.     

Distinguishing between the partial-mapping preparation hypothesis and the failure-to-engage hypothesis of residual switch costs.

Lien, Ruthruff, Remington, & Johnston (2005) reported residual switch cost differences between stimulus–response (S–R) pairs and proposed the partial-mapping preparation (PMP) hypothesis, which states that advance preparation will typically be limited to a subset of S–R pairs because of structural capacity limitations, to account for these differences. Alternatively, the failure-to-engage (FTE) hypothesis does not allow for differences in probability of advance preparation between S–R pairs within a set; it accounts for residual switch cost differences by assuming that benefits of advance preparation may differ between S–R pairs. Three Experiments were designed to test between these hypotheses. No capacity limitations of the type assumed by the PMP hypothesis were found for many participants in Experiment 1. In Experiments 2 and 3, no evidence was found for the dependency of residual switch cost differences between S–R pairs on response-stimulus interval that is predicted by the PMP hypothesis. Mixture-model analysis of reaction times distributions in Experiment 3 provided strong support for the FTE hypothesis over the PMP hypothesis. Simulation studies with a computational implementation of the FTE hypothesis showed that it is able to account in great detail for the results of the present study. Together, these results provide strong evidence against the PMP hypothesis and support the FTE hypothesis that advance preparation probabilistically fails or succeeds at the level of the task set. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Synthesis of transition metal-doped carbon xerogels by solubilization of metal salts in resorcinol–formaldehyde aqueous solution

The pore texture of carbon materials obtained from evaporative drying and pyrolysis of resorcinol–formaldehyde aqueous gels is controlled by the initial pH of the precursors solution. In order to produce transition metal-containing carbons with tailored texture, various metallic salts were dissolved in the precursors solution. When necessary, a complexing agent (HEDTA or DTPA) was added to render the metal ions soluble. Ni, Fe and Pd loaded carbon xerogels were synthesized and their pore texture was studied after evaporative drying and after pyrolysis. The carbon texture was also studied with regard to the nature of the metal and the amount of complexing agent. The solubilization of transition metal salts in the resorcinol–formaldehyde aqueous solution does not prevent the texture regulation, even though this texture control is influenced: the limits of the pH interval leading to micro–mesoporous carbon materials can slightly differ when a metal salt and/or a complexing agent are added. The pH range shift depends mainly on the amount and nature of the complexing agent, but also slightly on the nature of the metal ion. Nevertheless, the metal particles obtained are rather big (diameter > 15 nm). For catalytic applications, the metal dispersion must be enhanced, especially in the case of expensive metals.