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51.
Human peripheral blood monocytes were examined for migration across an endothelial cell monolayer in an in vitro vessel wall construct. Few monocytes invaded in the absence of a chemotactic gradient, despite significant adhesion to the endothelial monolayer. However, the addition of zymosan-activated human plasma to the lower compartment, to create a chemotactic gradient across the vessel wall, resulted in significantly enhanced monocyte migration. Pretreatment of the monocytes with monoclonal antibodies to thrombospondin (TSP) dramatically inhibited monocyte diapedesis into the vessel wall. The same treatment inhibited monocyte adhesion to endothelial cells in two-dimensional monolayer cultures as well as in vessel wall constructs (no chemotactic gradient). Of interest, however, the monoclonal antibodies had no inhibitory effect on monocyte migration into collagen gels devoid of endothelial cells in response to the same chemotactic gradient, suggesting the importance of TSP in monocyte-endothelial cell interactions. Monoclonal antibodies to fibronectin and normal mouse immunoglobulin G did not inhibit migration in this model of a vessel wall. Furthermore, monoclonal antibodies to TSP showed no inhibition of human peripheral blood neutrophil migration. Previous studies have shown that monocytes synthesize TSP and express this moiety on their surface. The present data suggest that monocytes may utilize TSP to interact with endothelial cells lining the vessel wall during diapedesis. 相似文献
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Cara L Booker Jennifer B Unger Stanley P Azen Lourdes Baezconde-Garbanati Brian Lickel C Anderson Johnson 《Nicotine & tobacco research》2007,9(11):1085-1094
The associations between stressful life events and smoking have been established among adolescents in the United States. However, whether these relationships are similar in adolescents from other non-Western cultures is unknown. Understanding these relationships in adolescents may help to provide opportunities to reduce the smoking rates in those cultures by providing positive coping methods that do not include smoking. In this longitudinal study, the associations between nine stressful life events scales and smoking behaviors were examined in a sample of Chinese adolescents. Six of these scales, positive school-related, negative school-related, positive family-related, positive peer-related, negative peer-related, and negative health-related had significantly different means among females and males. Among males, positive school-related stress was a protective factor for smoking susceptibility. Among females, positive school-related stress was a protective factor and negative school-related stress was a risk factor for lifetime smoking, and negative family-related stress was a risk factor for smoking susceptibility. Findings indicate that smoking among male adolescents in China may not be the result of stress; however, in females stress may contribute to the decision to smoke. Future directions are discussed. 相似文献
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POINT: AOP Will See Widespread Adoption Adrian Colyer, Rob Harrop, Rod Johnson, and Alexandre Vasseur AOP offers both the short--and long-term return on investment needed to ensure its widespread adoption. COUNTERPOINT: AOP Has Yet to Prove Its Value Danio Beuche and Cédric Beust Where is the proof that nonhierarchical modularization into aspects makes systems development cheaper or better? 相似文献
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During summer 1982, responses of lactating Holstein and Guernsey cows were measured by milk temperature recorded by a Digital Dataloger with thermocouples attached to Boumatic flow meters. Maximum air temperature and temperature-humidity index averaged 30.8 degrees C and 75.6 for July. Breed did not affect milk temperature, but within-breed milk temperature increased with production. In a second study, benefits of spray cooling were evaluated with 24 Holsteins in midlactation assigned randomly to two groups of 12 and maintained under loose-housing conditions. Spray nozzles were installed in the walkways and under the manger shade for the spray treatment group. Maximum temperature and temperature-humidity index during the spray study were 27 degrees C and 73.9. Rectal temperature taken following milking averaged less for treatment than control (38.8 versus 39.1 degrees C). Milk temperature was similar (37.8 versus 38.1 degrees C). Daily milk yield was .70 kg higher than controls. Milk temperature may provide reliable indication of climate stress similar to rectal temperature, and spray cooling improves cow comfort and lessens summer decline of milk production. 相似文献
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Constructions of particular random processes 总被引:1,自引:0,他引:1
Johnson G.E. 《Proceedings of the IEEE. Institute of Electrical and Electronics Engineers》1994,82(2):270-285
This paper reviews how to construct sets of random numbers with particular amplitude distributions and correlations among values. These constructions support both high-fidelity Monte Carlo simulation and analytic design studies. A variety of constructions is presented to free engineering models from “white or normal” limitations embodied in many current simulations. The methods support constructions of conventional stationary and normally distributed processes, nonstationary, nonnormal signal and interference waveforms, nonhomogeneous random scenes, nonhomogeneous volumetric clutter realizations, and snapshots of randomly evolving, nonhomogeneous scenes. Each case will have specified amplitude statistics, e.g., normal, log-normal, uniform, Weibull, or discrete amplitude statistics; and selected correlation, e.g., white, pink, or patchy statistics, clouds. or speckles. Sets of random numbers with correlation, nonstationarities, various tails for the amplitude distributions, and multimodal distributions can be constructed. The paper emphasizes aspects of probability theory necessary to engineering modeling 相似文献
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