Selective oxidation of key functional groups in cyanotoxins during drinking water ozonation

Chemical kinetics were determined for the reactions of ozone and hydroxyl radicals with the three cyanotoxins microcystin-LR (MC-LR), cylindrospermopsin (CYN) and anatoxin-a (ANTX). The second-order rate constants (k(O3)) at pH 8 were 4.1 +/- 0.1 x 10(5) M(-1) s(-1) for MC-LR, approximately 3.4 x 10(5) M(-1) s(-1) for CYN, and approximately 6.4 x 10(4) M(-1) s(-1) for ANTX. The reaction of ozone with MC-LR exhibits a k(O3) similar to that of the conjugated diene in sorbic acid (9.6 +/- 0.3 x 10(5) M(-1) s(-1)) at pH 8. The pH dependence and value of k(O3) for CYN at pH > 8 (approximately 2.5 +/- 0.1 x 10(6) M(-1) s(-1)) are similar to deprotonated amines of 6-methyluracil. The k(O3) of ANTX at pH > 9 (approximately 8.7 +/- 2.2 x 10(5) M(-1) s(-1)) agrees with that of neutral diethylamine, and the value at pH < 8 (2.8 +/- 0.2 x 10(4) M(-1) s(-1)) corresponds to an olefin. Second-order rate constants for reaction with OH radicals (*OH), k(OH) for cyanotoxins were measured at pH 7 to be 1.1 +/- 0.01 x 10(10) M(-1) s(-1) for MC-LR, 5.5 +/- 0.01 x 10(9) M(-1) s(-1) for CYN, and 3.0 +/- 0.02 x 10(9) M(-1) s(-1) for ANTX. Natural waters from Switzerland and Finland were examined for the influence of variations of dissolved organic matter, SUVA254, and alkalinity on cyanotoxin oxidation. For a Swiss water (1.6 mg/L DOC), 0.2, 0.4, and 0.8 mg/L ozone doses were required for 95% oxidation of MC-LR, CYN, and ANTX, respectively. For the Finnish water (13.1 mg/L DOC), >2 mg/L ozone dose was required for each toxin. The contribution of hydroxyl radicals to toxin oxidation during ozonation of natural water was greatest for ANTX > CYN > MC-LR. Overall, the order of reactivity of cyanotoxins during ozonation of natural waters corresponds to the relative magnitudes of the second-order rate constants for their reaction with ozone and *OH. Ozone primarily attacks the structural moieties responsible for the toxic effects of MC-LR, CYN, and ANTX, suggesting that ozone selectively detoxifies these cyanotoxins.     

Wilson-Cowan neural-network model in image processing

The neural-network model based on the theory proposed by Wilson and Cowan has been simulated by using digitized real images. Mathematically, the model is based on coupled nonlinear differential equations that describe the functional dynamics of cortical nervous tissue, and the model can operate in different dynamical modes, depending on coupling strengths. The model is shown to store images in reduced form and to recognize edges of an object. Examples of how the network processes input images are shown.     

A 119 GHz planar Schottky diode mixer for a space application

A planar single-ended GaAs Schottky diode mixer has been designed, built, and tested at 119 GHz. The mixer front end includes also a waveguide filter for image rejection, and a temperature compensated ring filter. Measurements at room temperature showed a conversion loss of 7 dB and a noise temperature of 900 K (SSB). At 100 K the measured noise temperature of the mixer was 500 K (SSB).     

When It Is Better to Give Than to Receive: Long-Term Health Effects of Perceived Reciprocity in Support Exchange.

The authors examined esteem enhancement theory and equity theory in explaining the long-term health status of men (n = 178) and women (n = 575). Baseline characteristics were measured in 1990-1993, intimate reciprocity in 1993, and recorded sickness absences for a 9-year period during 1993-2001. Among women, giving more support than receiving in intimate relationships positively affected subsequent health status, as indicated by fewer sickness absences. Among men, receiving more support than giving predicted fewer absences. These effects remained even after significant covariates were included in the model. Thus, women seem to benefit from giving support in intimate relationships, and men seem to benefit from receiving support in intimate relationships. The findings on women are in line with the esteem enhancement theory. No support was obtained for the equity theory. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A method for analysing the reliability of a transmission grid

The paper describes a probabilistic method for transmission grid security evaluation. Power system security is the ability of the power system to withstand sudden disturbances such as short circuits. The method presented here uses event and fault trees and combines them with power system dynamic simulations. Event trees model the substation protection and trip operations after line faults. Different event tree end states (fault duration, circuit breaker trips) are simulated with power system dynamic analysis program. The dynamic analysis results (power system post-fault states) are then classified into secure, alert, emergency and system breakdown. The probabilities, minimal cut sets and grid level importance measures (Fussell-Vesely, risk increase and decrease factors) are calculated for the total and partial system breakdown. In this way, the relative importance of the substation devices regarding to the system breakdown can be reached. Also the more and less likely contributing factors to system breakdown are received. With this method, an existing 400 kV transmission grid with its line fault and device failure statistics is analysed.     

Kernel-based spectral color image segmentation

In this work, we propose a new algorithm for spectral color image segmentation based on the use of a kernel matrix. A cost function for spectral kernel clustering is introduced to measure the correlation between clusters. An efficient multiscale method is presented for accelerating spectral color image segmentation. The multiscale strategy uses the lattice geometry of images to construct an image pyramid whose hierarchy provides a framework for rapidly estimating eigenvectors of normalized kernel matrices. To prevent the boundaries from deteriorating, the image size on the top level of the pyramid is generally required to be around 75 x 75, where the eigenvectors of normalized kernel matrices would be approximately solved by the Nystr?m method. Within this hierarchical structure, the coarse solution is increasingly propagated to finer levels and is refined using subspace iteration. In addition, to make full use of the abundant color information contained in spectral color images, we propose using spectrum extension to incorporate the geometric features of spectra into similarity measures. Experimental results have shown that the proposed method can perform significantly well in spectral color image segmentation as well as speed up the approximation of the eigenvectors of normalized kernel matrices.     

Regularized learning framework in the estimation of reflectance spectra from camera responses

For digital cameras, device-dependent pixel values describe the camera's response to the incoming spectrum of light. We convert device-dependent RGB values to device- and illuminant-independent reflectance spectra. Simple regularization methods with widely used polynomial modeling provide an efficient approach for this conversion. We also introduce a more general framework for spectral estimation: regularized least-squares regression in reproducing kernel Hilbert spaces (RKHS). Obtained results show that the regularization framework provides an efficient approach for enhancing the generalization properties of the models.     

Growth of immobilized DNA by polymerase: bridging nanoelectrodes with individual dsDNA molecules

We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.