Behaviour of oat globulins in lactic acid fermentation of oat bran

Oat bran fermentation (OBF) is used to produce non-dairy yogurt-type products. Such products may be designed being rich in probiotic bacteria and/or dietary-fibre. Oat bran is, however, also rich in proteins, especially 12 S globulins. Understanding the behaviour of globulins in OBF would thus offer a basis for further exploitation of proteins in the product design. The behaviour of oat globulins was monitored during a model OBF in order to study changes in protein solubility and possible protein hydrolysis. Proteins were extracted from OBF samples with a buffered and a non-buffered extraction procedure. The extracts were analyzed with SDS-PAGE and a Lowry assay. Combined effect of pH and NaCl-concentration on the solubility of oat globulin isolate was studied. The solubility of oat globulins decreased during OBF; this appeared as their shift from the salt-soluble fraction to the residual protein fraction. The shift in oat globulin solubility was due to the acidifying conditions present in OBF, which lead to the unfolding of globulins and also apparently induced protein aggregation. No major protein hydrolysis was observed during OBF.     

Comparing double-step and penalty-based semirecursive formulations for hydraulically actuated multibody systems in a monolithic approach

The simulation of mechanical systems often requires modeling of systems of other physical nature, such as hydraulics. In such systems, the numerical stiffness introduced by the hydraulics can become a significant aspect to consider in the modeling, as it can negatively effect to the computational efficiency. The hydraulic system can be described by using the lumped fluid theory. In this approach, a pressure can be integrated from a differential equation in which effective bulk modulus is divided by a volume size. This representation can lead to numerical stiffness as a consequence of which time integration of a hydraulically driven system becomes cumbersome. In this regard, the used multibody formulation plays an important role, as there are many different procedures for the constraint enforcement and different sets of coordinates to choose from. This paper introduces the double-step semirecursive approach and compares it with a penalty-based semirecursive approach in case of coupled multibody and hydraulic dynamics within the monolithic framework. To this end, hydraulically actuated four-bar and quick-return mechanisms are analyzed as case studies. The two approaches are compared in terms of the work cycle, energy balance, constraint violation, and numerical efficiency of the mechanisms. It is concluded that the penalty-based semirecursive approach has a number of advantages compared with the double-step semirecursive approach, which is in accordance with the literature.

     

From acacia honey monosaccharide content to a new external binary plasticizer applicable in aqueous whey protein films

Plasticizer is an essential adjuvant in food and pharmaceutical film coatings affecting the appearance, mechanical and permeation properties of the final coat. In the present study, film formation and plasticization of native whey proteins (potential future “green” coating agents for pharmaceuticals and food products), were studied with free isolated films. Special attention was paid to the effects of plasticizer, preheating and film forming solution pH on the mechanical stress–strain and moisture permeation properties of whey protein films. Glycerol, binary mixtures of fructose and glucose, and non-crystalline acacia honey were studied as external plasticizers. The type and amount of plasticizer affected the mechanical stress–strain properties of the whey protein films. A short preheating treatment of whey proteins prior to film casting resulted in mechanically strong films with a reduced elongation. The film forming properties of aqueous whey proteins could be modified by adjusting the pH above the isoelectric point of β-lactoglobulin prior to film coating. For effective plasticization, whey protein films required a high amount of monosaccharide containing plasticizer ranging from 80% to 120% (calculated from the protein weight). A new external binary plasticizer having the same ratio of monosaccharides as non-crystalline acacia honey (fructose and glucose 1.67:1) was found to be applicable in aqueous whey protein films.     

Free-field characterization via directional transmission through a nanoaperture

We propose a scheme based on extraordinary transmission of light through a single nanoaperture, surrounded by periodic corrugations, for direct characterization of focal-region optical fields with subwavelength-scale structure. We describe the design of the device on the basis of rigorous diffraction theory and fabricate a prototype using a process that involves electron beam lithography, dry etching, and template stripping. First experimental results performed with a transmission-type confocal optical microscope demonstrate the potential of the method.     

Influence of pulp bleaching and compatibilizer selection on performance of pulp fiber reinforced PLA biocomposites

This article focuses on the effect of pulp bleaching and emerging commercial compatibilizers on physical performance of pulp fiber reinforced poly(lactic acid) (PLA) biocomposites. Industrially bleached and unbleached hardwood kraft pulp fibers are treated with several additive types, and compounded with PLA to fiber content of 30 wt %. After injection molding, the produced biocomposites are evaluated by their mechanical performance and fiber–matrix adhesion. For selected materials, fiber surface and fiber properties are reflected to composite performance by analyzing the compositions, dimensions, and lignin coverage of original fibers, as well as fiber dispersion and dimensions after melt processing. As a conclusion, unbleached kraft pulp fibers provide significant improvement in physical properties of PLA/pulp fiber composites. Of the screened compatibilizers, epoxidated linseed oil has a clear positive effect on performance when bleached kraft pulp fibers are used. The improvements correspond to enhanced fiber–matrix adhesion and differences in remaining fiber length distributions. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136, 47955.     

A prognostic method for the embedded failure monitoring of solder interconnections with 1149.4 test bus architecture

This paper describes the feasibility of accurate low frequency measurements in predicting the breakdown of modern lead free ball grid array (BGA) interconnections. In these measurements, performed partly with 1149.4 analogue boundary scan, ceramic BGA modules measuring 15×15 mm in width, with 9×9 ball matrixes, were attached on an FR-4 printed wiring board (PWB) and thermally cycled over a temperature range of −40 to +125 °C. The condition of corner interconnections was monitored using the developed measurement methods and construction. In-situ measurements were performed with a datalogger during temperature cycling, accompanied with 1149.4 mixed-signal test bus measurements of corner interconnections performed between cycling intervals. In addition, the measurements were complemented by scanning acoustic microscopy and, X-ray. Monitoring corner interconnections by a simple, low-frequency voltage measurement method with embedded test constructions gives an early warning indication well before the electrical interconnection failures. Of two studied interconnection compositions, the ones with plastic core solder balls (PCSB) proved to be more reliable than the ones with 90/10 PbSn balls.     

Optimism and Pessimism as Predictors of Change in Health After Death or Onset of Severe Illness in Family.

The authors prospectively examined changes in health after a major life event (death or onset of severe illness in family) among 5,007 employees (mean age=44.8 years) whose optimism and pessimism levels were assessed in 1997 and major life events in 2000. Health was indicated by sickness absence days during a period covering 36 months prior to the event and 18 months after the event. Increase in sick days after the event was smaller and returned to the preevent level more quickly among highly optimistic individuals than among their counterparts with low optimism. Parallel changes were not observed in relation to pessimism. These findings suggest that optimism may reduce the risk of health problems and may be related to a faster recovery after a major life event. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Mind reading with regularized multinomial logistic regression

In this paper, we consider the problem of multinomial classification of magnetoencephalography (MEG) data. The proposed method participated in the MEG mind reading competition of ICANN’11 conference, where the goal was to train a classifier for predicting the movie the test person was shown. Our approach was the best among ten submissions, reaching accuracy of 68 % of correct classifications in this five category problem. The method is based on a regularized logistic regression model, whose efficient feature selection is critical for cases with more measurements than samples. Moreover, a special attention is paid to the estimation of the generalization error in order to avoid overfitting to the training data. Here, in addition to describing our competition entry in detail, we report selected additional experiments, which question the usefulness of complex feature extraction procedures and the basic frequency decomposition of MEG signal for this application.