Oxidation of microcystins by permanganate: reaction kinetics and implications for water treatment

A few genera of cyanobacteria produce toxins which contaminate drinking water resources. Microcystins (MC), widely reported cyanotoxins, cause acute and chronic toxicity effects in living beings including humans and warrant removal from drinking water. In the present study, unknown second-order rate constants for the reactions of microcystin-LR (MC-LR), -RR and -YR with potassium permanganate were determined at pH 6.2-8.2 and temperature 10-25 degrees C. The reaction of permanganate with MCs is second-order overall and first-order with respect to both permanganate and toxin. The second-order rate constant for the reaction of MC-LR with permanganate at pH 7 and 20 degrees C was 357.2+/-17.5M(-1)s(-1). The influence of pH on the oxidation process was not appreciable and the activation energy was 28.8 kJ mol(-1). Slightly higher reactivity with permanganate was found for MC-RR (418.0M(-1)s(-1)) and MC-YR (405.9M(-1)s(-1)). According to the results obtained, permanganate likely attacks the Adda moiety of the MC molecule. The oxidation of MCs in a natural surface water was also investigated. A permanganate dose of 1-1.25mgL(-1) was enough to reduce MCs concentration below the guideline value of 1microgL(-1). Permanganate oxidation is therefore a feasible option for microcystin removal during preoxidation processes. However, the oxidant dose must be carefully optimized in order to remove extracellular MCs without causing cell lysis (due to chemical stress) and further release of MCs.     

Parent’s self-reported indoor environment-related symptoms and health worry increase symptom reports among their children at school—Study in two independent populations

Little is known whether parent's indoor environment quality (IEQ)-related symptoms or health perceptions influence the risk of self- or parent-reported symptoms in their children. We assessed (i) the association of parents’ IEQ-related symptoms with IEQ-related symptoms in their children at school and (ii) whether parental IEQ-related health worry increases the risk for children's symptoms. We used two Finnish studies: a national, population-based survey of indoor air and related health problems (n = 611 parents) and a subset of survey for all primary school pupils (grade 3–6) and their parents in Helsinki, which also included school IEQ-related symptoms reported by children (n = 1617 parent-child dyads). In the school survey, parent's own symptoms increased strongly their reporting of their children's symptoms at school (aOR 4.0, 95% CI 2.7–6.0 for parents experiencing a lot of symptoms) and also symptoms reported by the child itself (aOR 2.2, 95% CI 1.5–3.1). Similar, but slightly weaker associations were seen with parental IEQ-related health worries. Results remained unchanged when adjusted for the IEQ of school buildings or parental and children's allergic diseases. Similar associations were seen in the national survey between parent's symptoms at work and child's symptoms at school. The results suggest that parents’ health perceptions may increase the reporting of children's IEQ-related symptoms even more than is typically seen for many indoor air contaminants.     

Friction Paths for Cubic Boron Nitride: An Ab Initio Study

Friction coefficients for cubic boron nitride were determined with the use of hybrid density functional B3LYP calculations. Two cluster models and an infinite periodic model were applied. Various contacts between duplicated surfaces and different sliding paths of the surfaces with respect to each other were taken into consideration, and friction coefficients were derived. The calculations suggest that three different sliding paths contribute to the friction, the friction coefficient for the periodic model being 0.21 at its lowest. This value is in agreement with previous experimental studies, where a friction coefficient of 0.1–0.2 has been measured for cubic boron nitride.     

Cutting thin sheet metal with a water jet guided laser using various cutting distances, feed speeds and angles of incidence

This research deals with the cutting of thin sheet metals at various distances, feed speeds and angles of incidence using a water jet guided laser. In the water jet guided laser process a laser beam is focused into a jet of water, which transmits the beam to the workpiece. This eliminates the need for any focus control. Nevertheless, most of its applications are in planar cutting where this advantage is not utilized. For the laser parameters, jet pressure and diameter in question, the value of 50 mm was found to be a fairly reliable upper limit to the cutting distance for both normal and inclined surfaces. In addition to the laser beam being absorbed partially by the water jet, the jet was found to be susceptible to disturbances. Specimen vibration caused by the water jet also impeded cutting a continuous kerf.     

Growth of immobilized DNA by polymerase: bridging nanoelectrodes with individual dsDNA molecules

We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.     

Spatial and temporal variations in water temperature in a high-altitude deep dimictic mountain lake (Nam Co), central Tibetan Plateau

Water temperature and the related thermal structure and stratification of a lake are very important to lake ecosystems because of their significant effects on the vertical exchanges of dissolved and particulate matter. In this study, we present high resolution, seasonal variations in water temperature at different depths of a large deep lake on the central Tibetan Plateau. The results show that Nam Co is a typical dimictic lake whose thermal stratification begins and ends in early June and early November, respectively. Increases in the water temperature during spring and the establishment of thermal stratification in the eastern small sub-basin occur approximately one month prior to the main basin which is likely caused by the different morphometry, different water transparency during spring, and the possible presence of a spring thermal bar. The Schmidt stability of the water column is directly controlled by surface water temperature. During the ice-covered period, the homogeneous water temperature exhibits a continuous increasing trend from approximately 0.5 °C to 3.5 °C. The daily mean surface water temperature of the main open lake area is highly correlated to the air temperature but shows a hysteresis effect of approximately 38 days, which shows the significant heat storage in such a large deep lake. Nam Co is a typical lake in this area in terms of its altitude, water depth and climatic conditions, so our results have broader significance for limnological and paleolimnological studies of similar lakes on the Tibetan Plateau.     

Novel Packet Switching for Green IP Networks

A green technology for reducing energy consumption has become a critical factor in ICT industries. However, for the telecommunications sector in particular, most network elements are not usually optimized for power efficiency. Here, we propose a novel energy‐efficient packet switching method for use in an IP network for reducing unnecessary energy consumption. As a green networking approach, we first classify the network nodes into either header or member nodes. The member nodes then put the routing‐related module at layer 3 to sleep under the assumption that the layer in the OSI model can operate independently. The entire set of network nodes is then partitioned into clusters consisting of one header node and multiple member nodes. Then, only the header node in a cluster conducts IP routing and its member nodes conduct packet switching using a specially designed identifier, a tag. To investigate the impact of the proposed scheme, we conducted a number of simulations using well‐known real network topologies and achieved a more energy‐ efficient performance than that achieved in previous studies.     

Predicting the spatial pattern of trees by airborne laser scanning

The spatial pattern of trees can be defined as a property of their location in relation to each other. In this study, the spatial pattern was summarized into three categories, regular, random, and clustered, using Ripley's L-function. The study was carried out at 79 sample plots located in a managed forest in Finland. The goal was to study how well the spatial pattern of trees can be predicted by airborne laser scanning (ALS) data. ALS-derived predictions were based upon individual tree detection (ITD), semi-individual tree detection (semi-ITD), and plot-level metrics calculated from the canopy height model, AREA. The kappa value for ITD was almost zero, which indicates no agreement. The semi-ITD and AREA methods performed better, although kappa values were only 0.34 and 0.24, respectively. It appears difficult to detect a particularly clustered spatial pattern.     

Altered developmental timing in early life stages of Antarctic krill (Euphausia superba) exposed to p,p'-DDE

Persistent organic pollutants (POPs) are persistent, toxic and bioaccumulative anthropogenic organic chemicals, capable of undergoing long range environmental transport to remote areas including the Antarctic. p,p′-dichlorodiphenyl dichloroethylene (p,p′-DDE) has been identified as a dominant POP accumulating in Antarctic krill (Euphausia superba), which is a key Southern Ocean species. This study examined the developmental toxicity of p,p′-DDE via aqueous exposure to Antarctic krill larvae. p,p′-DDE exposure was found to stimulate developmental timing in the first three larval stages of Antarctic krill, while extended monitoring of larvae after a five day exposure period had ended, revealed delayed inhibitory responses during development to the fourth larval stage. Stimulatory responses were observed from the lowest p,p′-DDE body residue tested of 10.1 ± 3.0 μmol/kg (3.2 ± 0.95 mg/kg) preserved wet weight, which is comparable to findings for temperate species and an order of magnitude lower than the exposure level found to cause sublethal behavioural effects in Antarctic krill. The delayed responses included increased mortality, which had doubled in the highest p,p′-DDE treatment (95 ± 8.9% mortality at 20 μg/L p,p′-DDE) compared to the solvent control (44 ± 11% mortality) 2 weeks after end of exposure. Development of surviving metanauplius larvae to calyptopis 1 larvae was delayed by 2 days in p,p′-DDE exposed larvae compared with untreated larvae. Finally, the developmental success of surviving p,p′-DDE exposed larvae was reduced by 50 to 75% compared to the solvent control (100% developmental success). The lowest observed effect concentration for all delayed effects was 1 μg/L, the lowest exposure concentration tested. These findings demonstrate the importance of delayed and indirect effects of toxicant exposure. Further, the findings of this study are important for environmental risk assessment of POPs in the Southern Ocean ecosystem and strongly highlight the significance of developmental endpoints for ecotoxicological testing.     

The role of utility models in patent filing strategies: evidence from European countries

We examine the role of utility models (UM) in patent filing strategies. With an extensive patent family data from European countries, we explore the structures and characteristics of patent families, which include UMs. A simple typology of patent families with UM members is introduced. We document that the geographical scope of most patent families with UM members is purely national, which is in line with the conventional view that the UM mechanism covers technologically and economically marginal inventions. However, the image of a UM as a signal of a minor invention is an oversimplification. Applicants exhibit a mixture of uses for the UM and there exists a subset of UM filings linked to inventions the inventive step of which meets or exceeds the threshold required for patent protection. Some UMs are members of international patent families, indicating that applicants may have some strategic motives to use UMs in international filing. The findings highlight that both types of IPR documents (UMs and patents) should be taken into account when working with data on patent families, analyzing patent filing strategies, and constructing patent-based indicators such as patenting propensities.