Analytical performance of polymer-based microfluidic devices fabricated by computer numerical controlled machining

A study comparing the electrophoretic separation performance attainable from microchips molded by masters fabricated using conventional CNC machining techniques with commercial microchips, wire imprinted microchips, and microchips from LIGA molding devices is presented. An electrophoresis-based detection system using fluorescence microscopy was used to determine the analytical utility of these microchips. The separation performance of CNC microchips was comparable to commercially available microchips as well as those fabricated from LIGA masters. The important feature of the CNC machined masters is that they have rapid design-to-device times using routinely available machining tools. This low-cost prototyping approach provides a new entry point for researchers interested in thermoplastic microchips and can accelerate the development of polymer-based lab-on-a-chip devices.     

Mitochondria directly influence fertilisation outcome in the pig

The mitochondrion is explicitly involved in cytoplasmic regulation and is the cell's major generator of ATP. Our aim was to determine whether mitochondria alone could influence fertilisation outcome. In vitro, oocyte competence can be assessed through the presence of glucose-6-phosphate dehydrogenase (G6PD) as indicated by the dye, brilliant cresyl blue (BCB). Using porcine in vitro fertilisation (IVF), we have assessed oocyte maturation, cytoplasmic volume, fertilisation outcome, mitochondrial number as determined by mtDNA copy number, and whether mitochondria are uniformly distributed between blastomeres of each embryo. After staining with BCB, we observed a significant difference in cytoplasmic volume between BCB positive (BCB+) and BCB negative (BCB-) oocytes. There was also a significant difference in mtDNA copy number between fertilised and unfertilised oocytes and unequal mitochondrial segregation between blastomeres during early cleavage stages. Furthermore, we have supplemented BCB- oocytes with mitochondria from maternal relatives and observed a significant difference in fertilisation outcomes following both IVF and intracytoplasmic sperm injection (ICSI) between supplemented, sham-injected and non-treated BCB- oocytes. We have therefore demonstrated a relationship between oocyte maturity, cytoplasmic volume, and fertilisation outcome and mitochondrial content. These data suggest that mitochondrial number is important for fertilisation outcome and embryonic development. Furthermore, a mitochondrial pre-fertilisation threshold may ensure that, as mitochondria are diluted out during post-fertilisation cleavage, there are sufficient copies of mtDNA per blastomere to allow transmission of mtDNA to each cell of the post-implantation embryo after the initiation of mtDNA replication during the early postimplantation stages.     

Chromium dinicocysteinate supplementation can lower blood glucose,CRP, MCP‐1, ICAM‐1, creatinine,apparently mediated by elevated blood vitamin C and adiponectin and inhibition of NFκB,Akt, and Glut‐2 in livers of zucker diabetic fatty rats

Chromium and cysteine supplementation can improve glucose metabolism in animal studies. This study examined the hypothesis that a cysteinate complex of chromium is significantly beneficial than either of them in lowering blood glucose and vascular inflammation markers in Zucker diabetic fatty (ZDF) rats. Starting at the age of 6 wk, ZDF rats were supplemented orally (daily gavages for 8 more weeks) with saline‐placebo (D) or chromium (400 μg Cr/Kg body weight) as chromium dinicocysteinate (CDNC), chromium dinicotinate (CDN) or chromium picolinate (CP) or equimolar L ‐cysteine (LC, img/Kg body weight), and fed Purina 5008 diet for 8 wk. ZDF rats of 6 wk age before any supplementations and onset of diabetes were considered as baseline. D rats showed elevated levels of fasting blood glucose, HbA₁, CRP, MCP‐1, ICAM‐1 and oxidative stress (lipid peroxidation) and lower adiponectin and vitamin C, when compared with baseline rats. In comparison to D group, CDNC group had significantly lower blood glucose, HbA₁, CRP, MCP‐1, ICAM‐1 and lipid peroxidation and increased vitamin C and adiponectin levels. CDN, CP or LC showed significantly less or no effect on these biomarkers. Only CDNC lowered blood creatinine levels in comparison to D. While CDN and CP had no effect, activation of NFκB, Akt and glucose transporter‐2 levels were decreased, insulin receptor substrate 1 (IRS‐1) activation increased in livers of CDNC‐rats. CDNC effect on glycemia, NFκB, Akt and IRS‐1 in liver was significantly greater compared with LC. Blood chromium levels did not differ between Cr‐groups. Exogenous vitamin C supplementation significantly inhibited MCP‐1 secretion in U937 monocytes cultured in high‐glucose‐medium. CDNC is a potent hypoglycemic compound with anti‐inflammatory activity apparently mediated by elevated blood vitamin C and adiponectin and inhibition of NFκB, Akt, and Glut‐2 and increased IRS‐1 activation in livers of type 2 diabetic rats.     

Preparation of ultrafine chalcopyrite nanoparticles via the photochemical decomposition of molecular single-source precursors

The synthesis and characterization of ultrafine CuInS2 nanoparticles are described. Ultraviolet irradiation was used to decompose a molecular single source precursor, yielding organic soluble approximately 2 nm sized nanoparticles with a narrow size distribution. UV-vis absorption, 1H and 31P{1H} NMR, and fluorescence spectroscopies and mass spectrometry were used to characterize decomposition of the precursors and nanoparticle formation. The nanoparticles were characterized by high-resolution transmission electron microscopy (HRTEM), scanning electron microscopy energy dispersive X-ray spectroscopy, powder X-ray diffraction (XRD), electron diffraction, inductively coupled plasma analysis, UV-vis absorption spectroscopy, and fluorescence spectroscopy. They have a wurzite-type crystal structure with a copper-rich composition. The hypsochromic shift in their emission band due to quantum confinement effects is consistent with the size of the nanocrystals indicated in the HRTEM and XRD analyses.     

Austempered Materials for Powertrain Applications

Austempered irons and steels offer the design engineer alternatives to conventional material/process combinations. Depending on the material and the application, Austempering may provide the following benefits to producers of powertrain components such as gears and shafts: ease of manufacturing, increased bending and/or contact fatigue strength, better wear resistance and enhanced dampening characteristics resulting in lower noise. Austempered materials have been used to improve the performance of powertrain components in numerous applications for a wide range of industries, from gears and shafts to clutch plates and crankshafts. This paper focuses on Austempered solutions for powertrain applications with an emphasis on gear and shaft solutions.     

Assessment of four different cathode materials at different initial pHs using unbuffered catholytes in microbial electrolysis cells

Nickel foam (NF), stainless steel wool (SSW), platinum coated stainless steel mesh (Pt), and molybdenum disulfide coated stainless steel mesh (MoS₂) electrodes have been proposed as catalysts for hydrogen gas production, but previous tests have primarily examined their performance in well buffered solutions. These materials were compared using two-chamber microbial electrolysis cells (MECs), and linear sweep voltammetry (LSV) in unbuffered saline solutions at two different initial pHs (7 and 12). There was generally no appreciable effect of initial pH on production rates or total gas production. NF produced hydrogen gas at a rate of 1.1 m³ H₂/m³·d, which was only slightly less than that using Pt (1.4 m³ H₂/m³·d), but larger than that obtained with SSW (0.52 m³ H₂/m³·d) or MoS₂ (0.67 m³ H₂/m³·d). Overall hydrogen gas recoveries with SSW (29.7 ± 0.5 mL), MoS₂ (28.6 ± 1.3 mL) and NF (32.4 ± 2 mL) were only slightly less than that of Pt (37.9 ± 0.5 mL). Total energy recoveries, based on the gas produced versus electrical energy input, ranged from 0.75 ± 0.02 for Pt, to 0.55 ± 0.02 for SSW. An LSV analysis showed no effect of pH for NF and Pt, but overpotentials were reduced for MoS₂ and SSW by using an initial lower pH. At cathode potentials more negative than −0.85 V (vs Ag/AgCl), NF had lower overpotentials than the MoS₂. These results provide the first assessment of these materials under practical conditions of high pH in unbuffered saline catholytes, and position NF as the most promising inexpensive alternative to Pt.     

Reservoir Inflow Monitoring for Improved Management of Treated Water Quality—A South Australian Experience

Large tributary inflows into water supply reservoirs caused by heavy catchment rain may be of concern due to problems associated with high levels of natural organic matter (NOM) present in the inflows. The movement of these inflows within a reservoir is dependent on its relative density to the receiving waters. For example, if the inflow is denser (colder) than the recipient water it will travel along the base of the reservoir as an underflow and can penetrate as far as the dam wall water off-take to a water treatment plant (WTP). Field studies were conducted to track the passage of underflows through two South Australian reservoirs, Little Para and Myponga. Samples were collected before and during storm event inflows and analyses undertaken to determine NOM concentration, alum demand, disinfection by-product formation potential, and quality of the water. We demonstrate that by monitoring the movement of inflows into reservoirs, combined with changes in reservoir off-take levels, that the risk of NOM entering a water treatment plant can be reduced which in turn will lower water treatment costs by reducing alum dosing levels and lessen the risk to human health by reducing disinfection by-product formation.     

Application of nanosilver surface modification to RO membrane and spacer for mitigating biofouling in seawater desalination

Biofouling is one the most critical problems in seawater desalination plants and science has not yet found effective ways to control it. Silver compounds and ions are historically recognized for their effective antimicrobial activity. Nanosilver particles have been applied as a biocide in many aspects of disinfection, including healthcare products and water treatment. This study proposes an innovative biofouling control approach by surface modification of the RO membrane and spacer with nanosilver coating. A chemical reduction method was used for directly coating nanosilver particles on the membrane sheet and spacer. The surface-modified membrane and spacer were tested for their antifouling performance in a cross-flow flat-sheet membrane cell, which is a part of a pilot plant in Wukan desalination plant. The silver-coating membranes and spacers, along with an unmodified membrane sheet, were tested in the membrane cell and compared on the basis of their antifouling performance. Permeate flux decline and salt rejection was continuously monitored through the testing period. Meanwhile regrowth of microbial populations on the membrane cell was quantified by a unique microbial counting every three to four days. The results showed that both silver-coated membrane (Ag-cM) with uncoated spacer and silver-coated spacer (Ag-cS) with uncoated membrane perforemed better than the unmodified membrane and spacer (Un-MS), in terms of much slower decrease in permeate flux and TDS rejection. However, the effect of silver-coated spacer on antimicrobial activity was more lasting. In the silver-coated spacer test, there was almost no multiplication of cells detected on the membrane during the whole testing period. Besides, the cells adhering to the membrane seemed to lose their activity quickly. According to the RO performance and microbial growth morphology, the nanosilver coating technology is valuable for use in biofouling control in seawater desalination.