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101.
Two-dimensional device simulations that confirm that the side-gating effect in GaAs MESFETs occurs on semi-insulating substrates containing hole traps are discussed. A negative voltage applied on a side gate, a separate n-type doped region, causes an increase in the thickness of the negatively charged layer at the FET channel interface in the substrate, through hole emission from hole traps. The FET channel current is modulated by the electron depletion of the n-type channel, which results from the compensation for the extension of the negatively charged layer at the n-i interface into the i-substrate containing hole traps. The magnitude of the drain current reduction is determined by the total acceptor concentration in the substrate and the donor concentration of the channel. However, the magnitude is independent of the side-gate distances 相似文献
102.
Kuwae S Ohda T Tamashima H Miki H Kobayashi K 《Journal of Bioscience and Bioengineering》2005,100(5):502-510
Antithrombin is a serine protease inhibitor that inactivates several coagulation proteases, primarily thrombin and factor Xa. The Chinese hamster ovary (CHO) cell line transfected with a vector expressing recombinant human antithrombin (rAT) and a selectable marker, glutamine synthetase (GS), was cultivated in a 2-l fed-batch culture process using serum-free, glutamine-free medium. To maximize the rAT yield, effects of culture pH, balanced amino acid feeding, and an increased glutamate concentration on cell metabolism and rAT production were investigated. When cells were grown at pH values of 6.6, 6.8, 7.0, and 7.2, the maximum cell density and maximum lactate concentration decreased with decreasing pH. The highest production level of rAT was obtained at culture pH 6.8 due to the extended culture lifetime. Compared to the imbalanced amino acid feeding at culture pH 6.8, the balanced amino acid feeding increased the amount of rAT activity by 30% as a result of an increased viable cell number. A decrease in the specific glucose consumption rate (q(Glc)) with increasing culture time was observed in all the above-mentioned experiments, while the glucose concentration was maintained above 0.7 g l(-1). In addition, a decrease in the specific rAT production rate (q(rAT)) was observed after the depletion of lactate in the late cultivation stage. Taken together, these results suggest that the reduced availability of cellular energy caused by the decrease in q(Glc) and depletion of lactate led to the decrease in q(rAT). This decrease in q(rAT) was partially prevented by increasing the residual glutamate concentration from 1 mM to 7 mM, thus resulting in an additional 30% increase in the amount of rAT activity. The optimized fed-batch culture process yielded 1.0 g l(-1) rAT at 287 h of cultivation. 相似文献
103.
Kubota K Kasuga F Iwasaki E Inagaki S Sakurai Y Komatsu M Toyofuku H Angulo FJ Scallan E Morikawa K 《Journal of food protection》2011,74(10):1592-1598
Most cases of acute gastroenteritis and foodborne disease are not ascertained by public health surveillance because the ill person does not always seek medical care and submit a stool sample for testing, and the laboratory does not always test for or identify the causative organism. We estimated the total burden of acute gastroenteritis in Miyagi Prefecture, Japan, using data from two 2-week cross-sectional, population-based telephone surveys conducted in 2006 and 2007. To estimate the number of acute gastroenteritis illnesses caused by Campylobacter, Salmonella, and Vibrio parahaemolyticus in Miyagi Prefecture, we determined the number of cases for each pathogen from active laboratory-based surveillance during 2005 to 2006 and adjusted for seeking of medical care and submission of stool specimens by using data from the population-based telephone surveys. Monte Carlo simulation was used to incorporate uncertainty. The prevalence of acute gastroenteritis in the preceding 4 weeks was 3.3% (70 of 2,126) and 3.5% (74 of 2,121) in the winter and summer months, yielding an estimated 44,200 episodes of acute gastroenteritis each year in this region. Among people with acute gastroenteritis, the physician consultation rate was 32.0%, and 10.9% of persons who sought care submitted a stool sample. The estimated numbers of Campylobacter-, Salmonella-, and V. parahaemolyticus -associated episodes of acute gastroenteritis were 1,512, 209, and 100 per 100,000 population per year, respectively, in this region. These estimates are significantly higher than the number of reported cases in surveillance in this region. Cases ascertained from active surveillance were also underrepresented in the present passive surveillance, suggesting that complementary surveillance systems, such as laboratory-based active surveillance in sentinel sites, are needed to monitor food safety in Japan. 相似文献
104.
105.
Rong Zhang Kaoru Shimada Shingo Mabu Kotaro Hirasawa 《IEEJ Transactions on Electrical and Electronic Engineering》2014,9(4):398-406
Attribute selection is a technique to prune less relevant information and discover high‐quality knowledge. It is especially useful for the classification of a large database, because the preprocessing of data increases the possibility that predictor attributes given to the mining algorithm become more relevant to the class attribute. In this paper, a method to acquire the optimal attribute subset for the genetic network programming (GNP) based class association rule mining has been proposed, and this attribute selection process using genetic algorithm (GA) leads to a higher accuracy for classification. Class association rule mining through GNP is conducted with a small subset of data rather than the original large number of attributes; thus simple but important rules are obtained for classification while the local optimal problem is avoided. Simulation results with educational data show that the classification accuracy is largely improved from 52.73 to 74.54%, when classification is made using the optimal attribute subset. © 2014 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc. 相似文献
106.
Fujita K Miyamura T Sano M Kato I Takegawa K 《Journal of Bioscience and Bioengineering》2002,93(6):614-617
Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) has transglycosylation activity, and high-mannose-type oligosaccharides are transferred to suitable glycosides as acceptor substrates. The acceptor specificity of Endo-A-catalyzed transglycosylation toward various disaccharides was investigated. To identify an effective acceptor for the transglycosylation by Endo-A, the reaction was carried out using various disaccharides. Endo-A transferred high-mannose-type oligosaccharides more efficiently to beta-linked disaccharides (cellobiose, gentiobiose, sophorose, and laminaribiose) than to alpha-linked disaccharides (isomaltose, maltose, nigerose, kojibiose, and trehalose) as acceptor substrates. The transglycosylation products, (Man)6GlcNAc-Glc-beta-Glc, were more rapidly hydrolyzed than (Man)6GlcNAc-Glc-alpha-Glc. These results indicate that Endo-A recognizes the anomeric configuration of the acceptor substrates, and beta-linked glycosides are suitable for the synthesis of transglycosylation products. 相似文献
107.
Shinji Kubo Masatoshi Futakawa Ikuo Ioka Kaoru Onuki Akihisa Yamaguchi 《International Journal of Hydrogen Energy》2013
Very harsh environments exist in the iodine–sulfur process for hydrogen production. Structural materials for sulfuric acid vaporizers and concentrators are exposed to high-temperature corrosive environments. Immersion tests were carried out to evaluate the corrosion resistance of ceramics and to evaluate corrosion-resistant metals exposed to environments of aqueous sulfuric acids at temperatures of 320, 380, and 460 °C, and pressure of 2 MPa. The aqueous sulfuric acid concentrations for the temperatures were 75, 85, and 95 wt%, respectively. Ceramic specimens of silicon carbides (SiC), silicon-impregnated silicon carbides (Si–SiC), and silicon nitrides (Si3N4) showed excellent corrosion resistance from weight loss measurements after exposure to 75, 85, and 95 wt% sulfuric acid. High-silicon irons with silicon content of 20 wt% showed a fair measure of corrosion resistance. However, evidence of crack formation was detected via microscopy. Silicon enriched steels severely suffered from uniform corrosion with a corrosion rate in 95 wt% sulfuric acid of approximately 1 g m−2 h−1. Among the tested materials, the ceramics SiC, Si–SiC, and Si3N4 were found to be suitable candidates for structural materials in direct contact with the considered environments. 相似文献
108.
M Utsumi K Moriguchi H Takahashi C Kinoshita A Togari M Mizutani N Ohno 《Journal of electron microscopy》1999,48(2):139-145
The ultrastructure of the parathyroid gland (PTG) of the UM-X7.1 cardiomyopathic hamster (the UM-X7.1) was qualitatively and quantitatively compared with that of the normal hamster. In the UM-X7.1, the Golgi apparatus and rER were well developed compared with those of the control hamster. Morphometric analysis revealed that the mean values of the Golgi apparatus, rER and large vacuolar bodies were significantly greater in the UM-X7.1 than in the normal hamster and the mean value of lipid droplets was significantly less in the UM-X7.1 than in the normal hamster. Ultrastructurally, we consider that in the UM-X7.1, the synthesis and release of the parathyroid hormone may be activated by an excessive amount of circulating catecholamine, and the functional activity of the PTG may be higher than that in the normal hamster. 相似文献
109.
110.
When the motor speed is reduced by using a regenerative brake, the mechanical energy of rotation is converted to electrical energy. When the regenerative torque is large, the corresponding current increases so that the copper loss also becomes large. On the other hand, the damping effect of rotation increases with time when the regenerative torque is small. In order to use this limited energy effectively, the optimal regenerative torque should be ascertained in order to maximize the regeneration of electrical energy. This paper proposes a methodology, based on the variational method, for the design of regenerative torque for an induction motor in order to maximize the regenerative electric energy. Similarly, the optimal torque for acceleration is derived in order to minimize the driving energy. Finally, an efficient motor drive system with the proposed optimal torque and a power storage system stabilizing the DC link voltage will be proposed. The effectiveness of the proposed methods is illustrated by simulations and experiments. © 2010 Wiley Periodicals, Inc. Electr Eng Jpn, 173(1): 41–50, 2010; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.21010 相似文献