全文获取类型
收费全文 | 1671篇 |
免费 | 49篇 |
国内免费 | 6篇 |
专业分类
电工技术 | 144篇 |
综合类 | 6篇 |
化学工业 | 558篇 |
金属工艺 | 39篇 |
机械仪表 | 67篇 |
建筑科学 | 35篇 |
能源动力 | 48篇 |
轻工业 | 148篇 |
水利工程 | 5篇 |
石油天然气 | 2篇 |
无线电 | 110篇 |
一般工业技术 | 299篇 |
冶金工业 | 58篇 |
原子能技术 | 44篇 |
自动化技术 | 163篇 |
出版年
2022年 | 11篇 |
2021年 | 17篇 |
2019年 | 13篇 |
2018年 | 14篇 |
2017年 | 11篇 |
2016年 | 31篇 |
2015年 | 29篇 |
2014年 | 47篇 |
2013年 | 78篇 |
2012年 | 55篇 |
2011年 | 85篇 |
2010年 | 62篇 |
2009年 | 73篇 |
2008年 | 85篇 |
2007年 | 74篇 |
2006年 | 68篇 |
2005年 | 62篇 |
2004年 | 64篇 |
2003年 | 48篇 |
2002年 | 56篇 |
2001年 | 37篇 |
2000年 | 36篇 |
1999年 | 44篇 |
1998年 | 34篇 |
1997年 | 41篇 |
1996年 | 24篇 |
1995年 | 31篇 |
1994年 | 18篇 |
1993年 | 18篇 |
1992年 | 19篇 |
1991年 | 19篇 |
1990年 | 19篇 |
1989年 | 27篇 |
1988年 | 29篇 |
1987年 | 27篇 |
1986年 | 26篇 |
1985年 | 26篇 |
1984年 | 19篇 |
1983年 | 17篇 |
1982年 | 30篇 |
1981年 | 22篇 |
1980年 | 22篇 |
1979年 | 20篇 |
1978年 | 15篇 |
1977年 | 21篇 |
1976年 | 13篇 |
1974年 | 10篇 |
1967年 | 13篇 |
1966年 | 11篇 |
1965年 | 13篇 |
排序方式: 共有1726条查询结果,搜索用时 46 毫秒
81.
Chien DN Tanaka M Tanaka K 《Journal of the Optical Society of America. A, Optics, image science, and vision》2002,19(8):1649-1657
The scattering phenomenon from an arbitrary-shaped end of a asymmetrical slab waveguide for the cases of TE and TM guided modes is simulated by means of boundary integral equations that are called guided-mode extracted integral equations. The integral equations that we derive can be solved by the conventional boundary-element method. Numerical results are presented for problems of three-layer asymmetrical waveguides with tilted ends. The reflection coefficient, reflected and scattered powers, and radiation patterns are calculated numerically for the cases of incident TE and TM guided modes. 相似文献
82.
Honda Y Kitaoka M Sakka K Ohmiya K Hayashi K 《Journal of Bioscience and Bioengineering》2002,93(3):313-317
The kinetic parameters of Cex, a family 10 xylanase from Cellulomonas fimi, were determined at various pH levels using soluble birchwood xylan (BWX) as a natural polymeric substrate along with three other synthetic aryl-beta-D-xylobioside substrates. Using BWX, a high level of substrate inhibition was observed which increased with decreasing pH. In contrast, typical Michaelis-Menten-type profiles were obtained using the three aryl-beta-D-xylobiosides as substrates. The k(cat) values determined using o-nitrophenyl-beta-D-xylobioside did not change as the pH increased, whereas the k(cat) values obtained with BWX, phenyl-beta-D-xylobioside and p-nitrophenyl-beta-D-xylobioside decreased, suggesting that the presence of an ortho nitro group affects the activity displayed by Cex. These differences were not observed with XynB from Clostridium stercorarium F9, a member of the same family of xylanases as Cex. These results indicate that a careful evaluation is required when employing substituted aryl-beta-D-xylobiosides in the characterization of xylanases. 相似文献
83.
Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp. 总被引:1,自引:0,他引:1
The eicosapentaenoic acid (EPA) synthesis gene cluster from an EPA-producing bacterium, Shewanella sp. SCRC-2738, was cloned into a broad-host range vector, pJRD215, and then introduced into a marine cyanobacterium, Synechococcus sp. NKBG15041c, by conjugation. The transconjugant cyanobacteria produced 3.7±0.2% (2.24±0.13 mg/L) EPA (n-3) and 2.5 ±0.2%
(1.49±0.06 mg/L) eicosatetraenoic acid (n-3) of the total fatty acids when the cells were cultured at 23°C at a light intensity
of 1,000–1,500 Lux. The EPA and eico-satetraenoic acid contents of the cells were increased to 4.6±0.6% (3.86±1.11 mg/L) and
4.7±0.3% (3.86±0.82 mg/L), and 7.5±0.3% (1.76±0.10 mg/L) and 5.1±0.2% (1.19±0.06 mg/L) when they were cultured at low temperature
(18°C) and at lower light intensity (40 Lux), respectively. 相似文献
84.
T Nishida TP Huang A Seiyama E Hamada W Kamiike S Ueshima H Kazuo H Matsuda 《Canadian Metallurgical Quarterly》1998,115(2):412-420
BACKGROUND & AIMS: Endothelin 1 is considered to be an important regulator of sinusoidal blood flow and increases during endotoxemia. The purpose of this study was to investigate the role of endothelin 1 in hepatic microcirculation, oxygen transport, and liver injury during endotoxemia. METHODS: Male Sprague-Dawley rats were continuously infused with 2.5 mL/h of saline, 0.8 mg . kg-1 . h-1 of lipopolysaccharide (LPS), 3 mg . kg-1 . h-1 of BQ-485, an endothelin A-receptor antagonist, or LPS plus BQ-485 for 7 hours. RESULTS: BQ-485 infusion had no significant effect on hepatic microcirculation and liver injury. LPS increased the plasma levels of aspartate aminotransferase (AST) and total bilirubin and decreased the hepatic adenosine triphosphate (ATP) level and bile flow rate. LPS + BQ-485 infusion further increased the plasma levels of AST and total bilirubin and decreased the bile flow rate and the hepatic ATP level. Dual-spot microspectroscopy revealed mild decreases in sinusoidal erythrocyte velocity and oxygen transport in the LPS group and profound decreases in these parameters in the LPS + BQ-485 group. Histological examinations revealed massive necrotic changes in the pericentral regions of the LPS + BQ-485 group. CONCLUSIONS: These results suggest that blockade of endothelin A receptors disturbs hepatic microcirculation and oxygen transport and aggravates the necrotic injury induced by endotoxin. 相似文献
85.
86.
87.
Sugio T Inoue T Kitano Y Takeuchi F Kamimura K 《Journal of Bioscience and Bioengineering》2004,98(2):85-91
A mesophilic, mixotrophic iron-oxidizing bacterium strain OKM-9 uses ferrous iron as a sole source of energy and L-glutamate as a sole source of cellular carbon. Uptake of L-glutamate into OKM-9 cells is absolutely dependent on ferrous iron oxidation. Thus, the Fe(2+)-dependent L-glutamate uptake system of strain OKM-9 is crucial for the bacterium to grow mixotrophically in iron medium with L-glutamate. The relationship between iron oxidation and L-glutamate transport activities was studied. Iron oxidase containing cytochrome a was purified 9-fold from the plasma membrane of OKM-9. A purified iron oxidase showed one rust-colored band following disc gel electrophoresis after incubation with Fe(2+). The Fe(2+)-dependent L-glutamate transport system was also purified 14.5-fold from the plasma membrane using the same purification steps as for iron oxidase. Fe(2+)-dependent L-glutamate and L-cysteine uptake activities of OKM-9 were 0.36 and 0.24 nmol/mg/min, respectively, when a concentration of 18 mM of these amino acids was used as a substrate. Both uptake activities were completely inhibited by potassium cyanide (KCN), suggesting that cytochrome a in the iron oxidase is involved in the transport process. The iron-oxidizing activity of strain OKM-9 was activated 1.7-fold by 80 mM L-glutamate. In contrast, the activity was noncompetitively inhibited by L-cysteine. The Michaelis constant of iron oxidase for Fe(2+) was 12.6 mM and the inhibition constant for L-cysteine was 41.6 mM. A marked inhibition of iron oxidase by 50 mM L-cysteine was completely reversed by the addition of 60 mM L-glutamate. The results suggest the possibility that iron oxidase has a binding site for L-cysteine and the cysteine first bound to the iron oxidase was replaced by the added L-glutamate. 相似文献
88.
Tanaka K Tanaka M 《Journal of the Optical Society of America. A, Optics, image science, and vision》2004,21(12):2344-2352
A new type of long narrow aperture in a pyramidal structure on a thick metallic screen is proposed, and optical wave scattering by this structure is simulated. This aperture structure provides high emission intensity and small spot size simultaneously through excitation of the surface plasmon polaritons on the sidewalls of the pyramidal structure. Scattering of optical waves by this structure in the thick metallic screen is solved numerically with a volume integral equation by generalized conjugate residual iteration and fast Fourier transformation. The basic characteristics of the near-field intensities of the aperture are investigated in detail. 相似文献
89.
Diffraction of an optical field by an aperture in a thick metallic screen is analyzed numerically by use of a three-dimensional volume integral equation together with a generalized conjugate residual method and fast Fourier transformation. Numerical results were validated by reciprocity and the independence of the results of the truncated discretized volume size used in numerical calculations. Near and far fields of square, circular, and triangular apertures in a thick screen are obtained numerically. Some of the numerical results obtained in the present study agree with previously reported experimental results. The surface plasmon polaritons excited on the sidewalls of the aperture can explain the basic characteristics of near-field distribution of apertures. The Bethe-Bouwkamp theory was found to be insufficient to explain the basic characteristics of the near field around the subwavelength aperture in a practical metallic screen. 相似文献
90.