考虑电动汽车用户满意度的微电网双层多目标优化调度

为了解决电动汽车(electric vehicle, EV)大规模接入微电网后,由于无序充电而导致的日负荷波动增大以及运行成本增高的问题,提出了一种考虑用户满意度的微电网双层多目标优化调度方案。方案将微电网日前优化调度过程分为配网层和负荷层,配网层以最小化日负荷方差和最低的系统运行成本为目标,制定了电动汽车聚合商(electric vehicle aggregator, EVA)调度策略。负荷层制定每辆电动汽车的充电策略,在EVA的调度计划下,按照配电网系统层的要求,最大限度地提高用户的综合满意度。通过多场景对比分析,结果表明所提出的优化模型在减少日负荷方差、提高系统经济性的同时,提升了用户综合满意度,增大了用户参与调度的积极性。     

一致持久的带外虚拟化系统

存储虚拟化能够全面提升存储区域网络的服务质量,而带外虚拟化与带内虚拟化相比具有性能高和扩展性好等优点．提出了运用按序操作、REDO日志以及日志完整性鉴别,共同保证带外虚拟化跨越系统崩溃或断电事故后仍旧可用;设计了一种基于关系模型的磁盘上虚拟化元数据组织方式,它具有可读性好和容易修改的优点;给出了通过分析磁盘分区表兼容典型遗留存储系统的有效方法,它具有庞大遗留系统零切割时间的特点．基于上述关键技术实现的原型系统,在典型实验中表现了很好的性能．     

GIS硬件平台建设实践

介绍了吉林省电力有限公司输变电生产管理地理信息系统（GIS）初期部署方案和所采用的负载均衡平台架构,并对其进行了分析,指出这种架构具有让所有用户直接访问统一的IP地址、不用人为干预协调也可以保证服务器的互相备份、能保证系统稳定等技术优势。     

基于LTCC带传输零点的带通滤波器

采用低温共烧陶瓷（LTCC）技术设计了带传输零点的带通滤波器,利用三维叠层结构实现了滤波器的小型化设计,借助HFSS仿真软件对滤波器参数进行优化仿真,改善了滤波器的传输特性,有效压缩了本振信号,满足了该带通滤波器设计指标和小型化的目的。     

ATM无源光网络中直流耦合突发模式光接收机分析

本文根据异步传输模式(ATM)无源光网络(APON)的实际要求,对直流耦合突发模式光接收机进行了理论分析,结果表明基于动态峰值检测的突发模式接收机其灵敏度代价和传输容量代价过高,而基于局部峰值检测的突发模式接收机则可较好满足APON的要求.     

基于双CPU技术的泵和马达控制系统的研究与设计

为获取泵和马达的静态与动态特性,设计了基于ADμC812和TMS320F206的双CPU结构的多传感器融合与控制系统。介绍了该系统的体系结构、硬件配置、接口电路、工作原理和工作流程。实现了传感器信息融合的高速高精度采集、复杂算法的大数据量实时计算以及模拟与开关量并有的多通道控制等功能,系统硬件结构简单。该系统作为泵、马达综合试验台的子系统,用于泵、马达性能试验。应用表明该系统能够满足试验要求,具有很好的可靠性和实时性。     

Study on destructions and reinforcement measures of mined constructions

Ground movement and deformation caused by underground mining would destroy the constructions located within subsidence area. Constructions would suffer different destructions with different location. The effect of ground deformation caused by underground mining to constructions was analyzed. Analyzed the destructive types of constructions in different ground movement and deformation. Taking a two-storey building for example, the walls were reinforced by computation before mining. To protect the construction the reinforcement measures to the construction before mining were studied.     

Generation and Identification of the Number of Copies of Exogenous Genes and the T-DNA Insertion Site in SCN-Resistance Transformation Event ZHs1-2

Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) causes an estimated economic loss of about USD 3 billion each year in soybean (Glycine max L.) production worldwide. Overexpression of resistance genes against SCN provides a powerful approach to develop SCN resistance cultivars in soybean. The clarification of molecular characterization in transformation events is a prerequisite for ecological risk assessment, food safety, and commercial release of genetically modified crops. Here, we generated transgenic events harboring the BCN (beet cyst nematode) resistance Hs1^pro−1 gene using the Agrobacterium-mediated method in soybean, evaluated their resistance to SCN infection, and clarified the molecular characterization of one of the transformation events. Five independent and stable inheritable transformation events were generated by an Agrobacterium-mediated transformation method. SCN resistance tests showed the average number of developed females per plant and female index (FI) in T4 ZHs1-1, ZHs1-2, ZHs1-3, ZHs1-4, and ZHs1-5 transformation events were significantly lower than that in the nontransgenic control. Among these, the ZHs1-2 transformation event had the lowest number of developed females per plant and FI. Southern hybridization showed the exogenous target Hs1^pro−1 gene was inserted in one copy and the Bar gene was inserted two copies in the ZHs1-2 transformation event. The exogenous T-DNA fragment was integrated in the reverse position of Chr02: 5351566–5231578 (mainly the Bar gene expression cassette) and in the forward position of Chr03: 17083358–17083400 (intact T-DNA, including Hs1^pro−1 and Bar gene expression cassette) using a whole genome sequencing method (WGS). The results of WGS method and Southern hybridization were consistent. All the functional elements of exogenous T-DNA fragments were verified by PCR using specific primer pairs in the T5 and T6 ZHs1-2 transformation events. These results demonstrated that the overexpression of Hs1^pro−1 gene enhanced SCN resistance, and provide an important reference for the biosafety assessment and the labeling detection in transformation event ZHs1-2.     

Development of a Multi-Epitope Vaccine for Mycoplasma hyopneumoniae and Evaluation of Its Immune Responses in Mice and Piglets

Mycoplasma hyopneumoniae (Mhp), the primary pathogen causing Mycoplasma pneumonia of swine (MPS), brings massive economic losses worldwide. Genomic variability and post-translational protein modification can enhance the immune evasion of Mhp, which makes MPS prone to recurrent outbreaks on farms, even with vaccination or other treatments. The reverse vaccinology pipeline has been developed as an attractive potential method for vaccine development due to its high efficiency and applicability. In this study, a multi-epitope vaccine for Mhp was developed, and its immune responses were evaluated in mice and piglets. Genomic core proteins of Mhp were retrieved through pan-genome analysis, and four immunodominant antigens were screened by host homologous protein removal, membrane protein screening, and virulence factor identification. One immunodominant antigen, {"type":"entrez-protein","attrs":{"text":"AAV27984.1","term_id":"53987783","term_text":"AAV27984.1"}}AAV27984.1 (membrane nuclease), was expressed by E. coli and named rMhp597. For epitope prioritization, 35 B-cell-derived epitopes were identified from the four immunodominant antigens, and 10 MHC-I and 6 MHC-II binding epitopes were further identified. The MHC-I/II binding epitopes were merged and combined to produce recombinant proteins MhpMEV and MhpMEVC6His, which were used for animal immunization and structural analysis, respectively. Immunization of mice and piglets demonstrated that MhpMEV could induce humoral and cellular immune responses. The mouse serum antibodies could detect all 11 synthetic epitopes, and the piglet antiserum suppressed the nuclease activity of rMhp597. Moreover, piglet serum antibodies could also detect cultured Mhp strain 168. In summary, this study provides immunoassay results for a multi-epitope vaccine derived from the reverse vaccinology pipeline, and offers an alternative vaccine for MPS.