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91.
92.
Strategies to encode or label small particles or beads for use in high-throughput screening and bioassay applications focus on either spatially differentiated, on-chip arrays or random distributions of encoded beads. Attempts to encode large numbers of polymeric, metallic or glass beads in random arrays or in fluid suspension have used a variety of entities to provide coded elements (bits)--fluorescent molecules, molecules with specific vibrational signatures, quantum dots, or discrete metallic layers. Here we report a method for optically encoding micrometre-sized nanostructured particles of porous silicon. We generate multilayered porous films in crystalline silicon using a periodic electrochemical etch. This results in photonic crystals with well-resolved and narrow optical reflectivity features, whose wavelengths are determined by the etching parameters. Millions of possible codes can be prepared this way. Micrometre-sized particles are then produced by ultrasonic fracture, mechanical grinding or by lithographic means. A simple antibody-based bioassay using fluorescently tagged proteins demonstrates the encoding strategy in biologically relevant media. 相似文献
93.
Hohkawa K Suda T Aoki Y Hong C Kaneshiro C Koh K 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2002,49(4):466-474
This paper presents results of a design study on a semiconductor coupled surface acoustic wave (SAW) convolver in which bi-directionally propagating SAWs, on a piezoelectric substrate with a high coupling coefficient, couple with bonded semiconductor diodes through multistrips. To obtain convolution signals with a high efficiency, we adopted a diode-balanced bridge structure for the nonlinear operation. We also found that the tapping pitches of the multi-strip electrodes have robustness against operation frequency variation and temperature-dependent variation on the delay of the SAW. We verified the effectiveness of the device in. a circuit simulation and an experiment on a test circuit, which was fabricated by using an epitaxial lift-off film-bonding process. 相似文献
94.
This research investigates the development of electronic commerce in Malaysia. It reviews the nation's e-commerce readiness and surveys local firms, a majority of them small- and medium-sized enterprises (SMEs), on their Web presence and e-commerce initiatives, management, capabilities and performance. Overall, while e-commerce in Malaysia is in its formative phases, a majority of firms in the survey sample have progressed beyond brochureware into the interactions phase; few have reached the e-commerce phase but many are planning to. They are driven primarily by marketing considerations and have seen the greatest impact of e-commerce on their marketing and overall business operations. Taking the lead are SMEs in IT/Internet services and solutions that see great opportunities in the nation's need to build the foundation layers for its emerging Internet economy. Most reluctant and least satisfied to date are SMEs in manufacturing that find the business-to-business sector largely undeveloped. The government can take some credits for its leadership in addressing the barriers to e-commerce development but the most significant barriers falls squarely within the private sector's domain. They will have to be overcome by entrepreneurial talents and management foresight. 相似文献
95.
A. Rastelli M. Stoffel G. Katsaros J. Tersoff U. Denker T. Merdzhanova G.S. Kar G. Costantini K. Kern H. von Knel O.G. Schmidt 《Microelectronics Journal》2006,37(12):1471-1476
We report on recent advances in the understanding of surface processes occurring during growth and post-growth annealing of strained islands which may find application as self-assembled quantum dots. We investigate the model system SiGe/Si(0 0 1) by a new approach based on “reading the footprints” which islands leave on the substrate during their growth and evolution. Such footprints consist of trenches carved in the Si substrate. We distinguish between surface footprints and footprints buried below the islands. The former allow us to discriminate islands which are in the process of growing from those which are shrinking. Islands with steep morphologies grow at the expense of smaller and shallower islands, consistent with the kinetics of anomalous coarsening. While shrinking, islands change their shape according to thermodynamic predictions. Buried footprints are investigated by removing the SiGe epilayer by means of selective wet chemical etching. Their reading shows that: (i) during post-growth annealing islands move laterally because of surface-mediated Si–Ge intermixing; (ii) a tree-ring structure of trenches is created by dislocated islands during their “cyclic” growth. This allows us to distinguish coherent from dislocated islands and to establish whether the latter are the result of island coalescence. 相似文献
96.
Internet-enabled cell phones typically accommodate only three to 12 text lines, and their design emphasizes portability. Web interaction has, so far, been a secondary concern. For the most part, automated techniques to address the feature gap between the desktop and these phonetops rely on the notion of transducing-translating HTML and images into formats compatible with small devices, which typically cannot handle HTML content. The authors have developed a Web browsing model that supports navigation and action in separate interfaces, To demonstrate the model, they created m-Links, a middleware proxy system that retrieves Web documents using HTTP, lets users navigate and apply services to Web content, and delivers a suitable user interface to a variety of small Web-capable wireless devices. 相似文献
97.
Haguenauer P Berger JP Rousselet-Perraut K Kern P Malbet F Schanen-Duport I Benech P 《Applied optics》2000,39(13):2130-2139
The optical characterization of a fiber-connected planar optics beam combiner dedicated to astronomical interferometry for two telescopes is presented. The beam combiner, fully integrated on a single 5 mm x 40 mm glass chip, is tested as the central part of an astronomical instrument. The single-mode waveguides are made by silver-ion-exchange technology upon glass substrates and provide spatial filtering, which improves the visibility measurement accuracy by selecting only the fundamental mode of the beams at the telescope focal plane. A global optical throughput of 43% is measured, and the sources of losses are identified and examined in detail. Solutions for improving this throughput are proposed. High and stable contrasts are obtained with a 1.55-mum laser diode (?96%) and with a white-light source (~92%) in the astronomical H filter (1.43 mum; 1.77 mum). The need for accurate control of differential instrumental polarization is demonstrated. In this context the intrinsic polarization-maintaining property of the planar optics component is characterized. This validation of the important potential uses of integrated planar optics should be valuable for future design of optical telescope arrays. 相似文献
98.
TEM images of thin foils with quasi-globular particles are examined by means of two methods of spatial statistics. The spatial arrangement of particle reference points is described by means of quadrat count statistics and by polygonal method (the analysis of the Voronoi mosaic generated by patterns of particle reference points). A good agreement between the both approaches is found, the polygonal method is more sensitive and its results are more conclusive. 相似文献
99.
Fifty methicillin-resistant Staphylococcus aureus (MRSA) initial isolates obtained from patients hospitalized in the orthopedic clinic of the Frankfurt University Hospital and 150 methicillin-sensitive Staphylococcus aureus (MSSA) isolates were investigated in this study to determine whether the Slidex Staph-Kit is capable of differentiating between MRSA and MSSA owing to its unique performance characteristics. The Slidex Staph-Kit is a combined latex hemagglutination test designed to detect clumping factor, protein A, and a specific surface immunogen for S. aureus. Clumping factor-positive strains cause erythrocytes sensitized with fibrinogen to hemagglutinate, thereby resulting in visible red clumps. S. aureus strains deficient in clumping factor agglutinate latex particles sensitized with specific antibodies against surface proteins of S. aureus, thereby resulting in visible white clumps. Our results demonstrate that white clumping has a 99% specificity as well as a 98% positive predictive value for MRSA. Clumping factor-negative MRSA, which have been reported to occur in several countries, are epidemic in the Frankfurt area and account for 80% of all MRSA initial isolates in the orthopedic clinic of the Frankfurt University Hospital. Genotyping of all MRSA isolates by macrorestriction analysis of chromosomal DNA revealed that 83% of clumping factor-negative MRSA are closely related to the "southern-German" epidemic strain. This is the first study demonstrating the Slidex Staph-Kit's capability for identifying epidemic clumping factor-negative S. aureus strains as methicillin resistant even prior to antimicrobial susceptibility testing. 相似文献
100.
S. K. Koh R. I. Stephens 《Fatigue & Fracture of Engineering Materials & Structures》1991,14(4):413-428
Abstract— ASTM A723 Q & T steel with a yield strength and ultimate strength of 1170 and 1262 MPa respectively was evaluated for mean stress-strain effects under smooth specimen axial strain controlled low cycle fatigue conditions with strain ratios R of −2, −1, 0, 0.5 and 0.75. Cycles to failure ranged from 15 to 105 . Cyclic stress-strain response based upon half-life hysteresis loop peaks were similar for all R ratios. Mean stress relaxation occurred for R ≠−1 only when plastic strain amplitudes were present and this occurred above total strain amplitudes of 0.005. Thus, mean stress relaxation was completely dependent upon cyclic plasticity. Mean strains did not affect low cycle fatigue life unless accompanied by half-life mean stress. Tensile mean stress was detrimental and compressive mean stress was beneficial and these effects only occurred at strain ampltidues below 0.005. Three different mean stress models were used to evaluate the low cycle fatigue data and the SWT log-log linear model best represented the data. These results can be used with the local notch strain fatigue life prediction methodology. 相似文献