The aim of this study was exploration of the genetic background of conjunctival melanoma (CM) and correlation with recurrent and metastatic disease. Twenty-eight CM from the Rotterdam Ocular Melanoma Study group were collected and DNA was isolated from the formalin-fixed paraffin embedded tissue. Targeted next-generation sequencing was performed using a panel covering GNAQ, GNA11, EIF1AX, BAP1, BRAF, NRAS, c-KIT, PTEN, SF3B1, and TERT genes. Recurrences and metastasis were present in eight (29%) and nine (32%) CM cases, respectively. TERT promoter mutations were most common (54%), but BRAF (46%), NRAS (21%), BAP1 (18%), PTEN (14%), c-KIT (7%), and SF3B1 (4%) mutations were also observed. No mutations in GNAQ, GNA11, and EIF1AX were found. None of the mutations was significantly associated with recurrent disease. Presence of a TERT promoter mutation was associated with metastatic disease (p-value = 0.008). Based on our molecular findings, CM comprises a separate entity within melanoma, although there are overlapping molecular features with uveal melanoma, such as the presence of BAP1 and SF3B1 mutations. This warrants careful interpretation of molecular data, in the light of clinical findings. About three quarter of CM contain drug-targetable mutations, and TERT promoter mutations are correlated to metastatic disease in CM. 相似文献
Activated carbon is a suitable adsorbent for removal of hydrogen sulphide from natural, synthesis or other product gases. The process depends predominantly on physical adsorption, though catalytic oxidation is also involved. During catalytic oxidation the H2S is converted in the presence of oxygen to elemental sulphur, which is adsorbed onto the internal surface of the activated carbon, thus leading to a sulphur load of up to 120% by weight. The oxidation rate depends on the partial pressure of both reactants, H2S and O2 and is largely controlled by the characteristics of the activated carbon. The activity of the catalyst can be improved by impregnating the activated carbon with promoters such as iron and iodine. The regeneration of spent carbon is currently carried out using hot gas desorption methods at temperatures around 450 °C. 相似文献
Sulfonated polystyrene latex particles were used as acid catalysts in the continuous inversion of sucrose at 50–70°C. The particles (surface charge 168 μeq/g) were confined in a stirred reactor with a semipermeable membrane; sucrose solution was pumped in, and product solution was pumped out. The catalytic activity of the particles was unchanged after 20 days continuous use. Variation of particle size (0.13 and 0.42 μm) and stirring rate showed that internal and external mass transfer was not a controlling factor. The kinetics were pseudo-first-order; the rate constant at 70°C was 2.30/N min as compared with 0.07/N min for macroporous sulfonated ion-exchange resin; the apparent activation energy was 111 kJ/mol as compared with 121 kJ/mol for the homogeneous acid-catalyzed reaction. The faster rate was attributed to the very great surface area, high charge density, and lack of internal diffusional resistance of the latex particles. The proposed mechanism comprised adsorption of sucrose on the particle surface, followed by inversion and desorption of product. 相似文献
The phase separation behavior of dilute polydisperse polymer solutions primarily depends on the partial concentration of each polymer component. So the equilibrium data obtained by turbidimetric titration with “calibration” fractions and their mixtures can be used for the construction of a solubility diagram (Claesson-diagram) which generally allows to describe fractionation processes. It can be shown that the characteristic features of precipitation fractionations can well be derived from the solubility diagram. This diagram then offers the possibility for a complete discussion of parameter variation (concentration, step of γ-variation, process-scheme, temperature, solvent/nonsolvent-system) on the efficiency of a fractionation step. On one hand these results correspond – as for the concentration variation – to well known experimentell experiences. On the other hand it is possible for the first time to give definite predictions for the influence of temperature and the solvent/nonsolvent system. The temperature selection can be shown to be inconsiderable for the separation effect. The usual characterization of solvents and nonsolvents as “good” or “poor” is absolutely insufficient, if not misleading, for there is no connection between these properties and the shape of the solubility diagram. Based on the analysis of the single parameters the optimum conditions for the preparation of narrow preparation of narrow precipitation fractions can be derived. 相似文献
The effect of screw wear on the performance of a 2.5 in. diameter extruder is studied with the aid of computer simulations. The effect of progressively increasing flight clearance on the extrusion of low density polyethylene, polypropylene and nylon 6/6 is presented. The remedial effect of increased screw speed and its side effects on melting behavior, solids content, extrudate temperature and power consumption are also described. 相似文献
In this report a survey is given on structure and properties of polyacrylamide homopolymers (PAAm) in solution. However the review is restricted to all those papers, where a molecular characterization of the polymers has been achieved as a basis to correlate this fundamental information with applicational properties.
Different polymerization methods are summarised in brief, the preparation and solution structure of long chain branched polyacrylamides as well as chemical modification reactions of linear PAAm are also mentioned. A number of experimental characterization methods (GPC, ultracentrifugation, intrinsic viscosity, and light scattering measurements) are described with special emphasis on the difficulties of the different procedures including some proposals for properly designed experimental techniques. The state of solution is discussed in view of experimental data obtained with different solvents. Moreover viscosity constant ø is calculated for aqueous solution and the unperturbed dimensions are estimated. All available data on cross correlations (e.g. [η] - M, S0 - M, 2>1/2 - M) are collected with the intention to give a survey of established relations and, comparing the given relationships, to suggest the reliable ones of them.
The phenomenon of long-term viscosity decrease of aqueous PAAm solutions has been investigated and discussed with regard to its molecular origin.
The viscoelastic properties are discussed in dependence on molecular weight, concentration, solvent quality, and shear rate (106 · s−1). Based on these data a simple equation was developed oped for the η0-c-M relationship, which can be applied to other polymer systems as well. It is further described that the elastic nature (first normal stress difference) may overwhelm the viscous nature (shear stress) at relatively low shear rates. This high elasticity can cause deviation from laminar flow conditions. Moreover, it can be demonstrated — based on instationary measurements as well as the comparison of steady shear flow with dynamic rheology — that energetic interactions (H-bonds) strongly influence the rheological behaviour. 相似文献
Yield as well as Contents and Composition of Fats and Proteins in Several Varieties and Types of Mustard at Distinctly Different Ecological Locations In a cultivation experiment extending over two years the yield of different varieties of Brassica juncea, Brassica nigra and Sinapis alba was examined. Fat content and protein content were determined and the correlations with different characteristics were calculated. Fatty acid composition of the oil showed that erucic acid content was distinctly influenced by the location and the year of cultivation. In all samples, whose erucic acid content showed large difference from average values for the variety, electrophoretic patterns of the proteins were found to be distinctly different, both with respect to number and distribution of the bands. 相似文献