Cytoplasmic mRNA for human triosephosphate isomerase is immune to nonsense-mediated decay despite forming polysomes

Nonsense codons between position 14 within the first exon and position 193 within the penultimate exon of the human gene for triosephosphate isomerase reduce mRNA abundance to 25% of normal. The reduction in abundance is due to the decay of newly synthesized mRNA that copurifies with nuclei. TPI mRNA that copurifies with cytoplasm is immune to decay. We show here that immunity is not due to the failure of nonsense-containing mRNA to form polysomes. This finding indicates that cytoplasmic mRNA, in contrast to nucleus-associated mRNA, may have lost one or more factors that are required for nonsense-mediated decay or gained one or more factors that confer immunity to nonsense-mediated decay.     

基于海量搜索历史数据的用户兴趣模型

针对互联网搜索引擎环境中,基于海量搜索历史数据分析用户兴趣的问题,提出一种改进的用户兴趣模型。该模型根据用户搜索的历史数据,结合向量空间模型（ VSM ）和TF-IDF算法,递归地回溯出用户兴趣权重列表。为解决用户兴趣变化和时间性能的问题,该模型引入时间遗忘机制进行动态更新,并在Hadoop分布式系统架构下利用MapReduce分布式编程模型进行实现。实验结果表明,改进的用户兴趣模型的查准率和召回率都能达到50',具有较好的可行性和可用性。     

基于USB的WSN测试与分析系统

实时监控无线传感器网络（WSN）从单个节点到整个网络的运行状态,是进行无线传感器各类研究及应用开发的关键技术之一,为了克服基于UART的数据传输所存在的速率瓶颈问题,设计了一种基于USB的WSN监测系统,主要包括侦听节点和监测分析系统两部分。侦听节点采用CC2531 USB Dongle,以被动侦听的方式获取无线传感器网络的数据包（符合IEEE802.15.4标准）,封装后通过USB接口上传至上位机监测分析系统。上位机监测系统,通过USB接口读取数据帧、完成帧信息存储、解析。实验结果表明该设计能够实现数据传输并以图形方式动态显示网络运行状态。本监测系统的设计为开展WSN的各类理论及实验研究提供了有力的分析工具。     

基于2D Mesh拓扑结构的NoC模拟器设计

片上网络模拟器的设计涉及到片上网络的拓扑结构、路由器结构、路由算法、性能分析等诸多方面.从NoC模拟器设计的角度,研究并讨论模拟器所采用的拓扑结构,路由器结构及数据包格式,介绍拓扑结构模拟、IP核模拟、路由模拟,并且用面向对象语言C++实现一个NoC模拟器系统.     

CD57+/CD28- T cells in untreated hemato-oncological patients are expanded and display a Th1-type cytokine secretion profile, ex vivo cytolytic activity and enhanced tendency to apoptosis

Protopolystoma (Monogenea, Polystomatidae) is strictly specific to the anuran amphibian genus Xenopus. The host group is characterised by a polyploid series in which chromosome numbers reflect diploid, tetraploid, octoploid and dodecaploid constitutions; the series is considered to have evolved through interspecies hybridisation and genome duplication. This study correlates information on host evolutionary relationships with patterns of parasite speciation and host specificity. Protopolystoma is restricted to one subgenus (Xenopus) with multiples of 36 chromosomes, and is absent from the subgenus Silurana (with multiples of 20 chromosomes). Molecular, biochemical and karyotype evidence distinguishes three subgroups within Xenopus. Representative species from each subgroup, Xenopus muelleri, Xenopus fraseri and Xenopus laevis, have been examined for polystomatid infection. Two species of Protopolystoma occur in each of these host species. In X. muelleri, the two Protopolystoma species reflect parasite co-speciation corresponding with the divergence of two sibling host species. Xenopus fraseri and X. laevis (both with 2n = 36 chromosomes) are implicated in the hybrid origin of two octoploid species, Xenopus wittei and Xenopus vestitus (both 2n = 72). The relationships of the Protopolystoma species in these Xenopus taxa reflect this presumed ancestry. Xenopus wittei carries two species of Protopolystoma, one shared with X. fraseri and the other shared with X. laevis. Xenopus vestitus carries a single species of Protopolystoma which is shared with X. laevis but there is no "heirloom" which reflects its hybrid origin involving X. fraseri. In addition to these shared parasite species which may reflect shared host genes, X. fraseri and X. laevis each carry separate species-specific Protopolystoma which do not occur in other Xenopus species even where there is evidence of common genetic information (as in the allopolyploid wittei and vestitus). This case study may be interpreted as indicating a powerful influence of host genetic factors on susceptibility to infection, host-specificity, and parasite speciation.     

氡及其子体运移仿真系统开发

基于.net开发平台,借助C讨论了仿真系统的软件实现过程,简述了系统的功能模块及关键技术实现.实现了氡及其子体运移机制研究的计算机化.     

Increased messenger RNA from protein synthesis inhibited human fibroblasts

Numerous reports have demonstrated that specific protein synthesis in response to specific inducers is markedly stimulated by a simultaneous brief exposure to protein synthesis inhibitors such as cycloheximide. This phenomenon is known as "superinduction" and is most often attributed to the accumulation of cytoplasmic messenger RNA during the inhibition period. Messenger RNA, as defined by rapid labeling, oligo (dt)-cellulose binding, and cell free protein synthesis stimulation was measured in cycloheximide treated human fibroblasts. In spite of a consistent 40% decrease in total polysomal 3H-uridine labeled RNA, a 1.5- to 2-fold increase in extractable mRNA was observed. These data provide direct evidence that protein synthesis inhibition stimulates the appearance of cytoplasmic mRNA and/or completely blocks its degradation and, are consistent with the hypothesis that mRNA accumulation partly underlies the superinduction phenomena.     

Subjective time experience in an intergenerational sample

The roles chronological age and gender play in subjective time experience were explored in a sample of 294 adult men and women. Subjective time experience (STE: the difference between subjective age and chronological age) was found to vary widely among individuals, with some being "accurate" (SA = CA), and others either "retarded" (SA less than CA) or "advanced" (SA greater than CA). Males were more retarded in STE than females at every point in the lifespan, and patterns of age differences in adulthood differed for the two sexes as well. The results suggest that chronological age may play a key role in transitions in STE, and that chronological age is more significant in the STE of women than in the STE of men.