Mismatch discrimination in oligonucleotide hybridization reactions using single strand binding protein. A surface plasmon fluorescence study

We describe the use of surface plasmon- and surface plasmon fieldenhanced fluorescence spectroscopy for the detection of hybridization reactions between surface-attached probe oligonucleotides and complement strands binding from solution. These targets, exhibiting different base mismatches relative to the probe 15-mer sequences, carry a fluorophore at their 5'-end thus allowing for sensitive detection and quantification of association, k_on, and dissociation, k_off, rate constants, as well as affinity constants, K_a. We demonstrate that by the competitive binding / replacement of single strand binding proteins the mismatch discrimination can be further enhanced.     

Feature integration across space, time, and orientation.

The perception of a visual target can be strongly influenced by flanking stimuli. In static displays, performance on the target improves when the distance to the flanking elements increases—presumably because feature pooling and integration vanishes with distance. Here, we studied feature integration with dynamic stimuli. We show that features of single elements presented within a continuous motion stream are integrated largely independent of spatial distance (and orientation). Hence, space-based models of feature integration cannot be extended to dynamic stimuli. We suggest that feature integration is guided by perceptual grouping operations that maintain the identity of perceptual objects over space and time. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Estimation of fixed-point temperatures - A practical approach

Phase transition temperatures can be used for thermometer calibrations in accordance with the ITS-90. For this the thermometer is inserted into a fixed-point cell filled with a highly pure substance. While the substance is melting or freezing the temperature inside the cell remains almost constant and the thermometer measures a plateau in the temperature curve. From this plateau the phase transition temperature needs to be estimated for the calibration of the thermometer. This can be done using different mathematical methods, taking into account various systematic deviations as well as reproducibilities of the results depending on the chosen method, the fixed-point material’s purity and above all the amount of fixed-point material. Hence this article presents results from measurements in miniature fixed-point cells filled with zinc of various purities. The plateau curves were measured at different heating rates and comparatively analysed using five different estimation methods.     

Hydrothermal catalytic production of fuels and chemicals from aquatic biomass

One of the promising avenues for biomass processing is the use of water as a reaction medium for wet or aquatic biomass. This review focuses on the hydrothermal catalytic production of fuels and chemicals from aquatic biomass. Two different regimes for conversion of aquatic biomass in hydrothermal conditions are discussed in detail. The first is hydrothermal liquefaction, and the second is hydrothermal gasification. The goals of these processes are to produce liquid‐fuel‐range hydrocarbons and methane or hydrogen, respectively. The catalytic upgrading of biocrude resulting from noncatalytic liquefaction and the stability and degradation of catalysts in high temperature water are also discussed. The review concludes with a brief discussion of the outlook for and opportunities within the field of hydrothermal catalytic valorization of biomass. Copyright © 2012 Society of Chemical Industry     

Advanced Alumina Composites Reinforced with Titanium-Based Alloys

New (inter)metallic-ceramic composites for high-temperature structural and functional applications are prepared via high-energy ball milling. During compaction by pressureless sintering, dense Al₂O₃/Ti-based alloy composites are formed that consist of inter-connected networks of the ceramic and the (inter)metallic phases. Ti-Al-V/Al₂O₃ and Ti-Al-Nb/Al₂O₃ composites show enhanced damage tolerance over monolithic Al₂O₃, i.e ., fracture toughnesses up to 5.6 MPa·m^0.5 and bending strengths up to 527 MPa. The resistance against abrasive wear is almost doubled with respect to monolithic Al₂O₃ ceramic. Electrical resistivity scales with the ceramic volume fraction and ranges between 0.3 mΩ·cm and 55.1 mΩ·cm, with only a weak temperature dependence ≤700°C.     

Raman investigation of single oxidized carbon nanotubes

The oxidation process of single-walled carbon nanotubes via nitric acid treatment was followed by IR-, UV-Vis-NIR, and single bundle Raman spectroscopy. The introduction of functional, oxygen-containing groups is revealed by an additional absorption band at 1725 cm⁻¹, characteristic of carbonyl stretch vibrations. No significant shift of the optical absorption bands could be detected after oxidation. The combination of atomic force microscopy and confocal scanning resonance-enhanced Raman microscopy was used to investigate thin bundles and, eventually, individual nanotubes in detail. These experiments enabled determination of the dependence of the Raman intensity of the G-line (around 1590 cm⁻¹) on the bundle height for both non-oxidized and oxidized tubes. The Raman cross-section of the oxidized tubes was found to be reduced by a factor of ˜4, compared to the pristine tubes. This observation is ascribed to all tubes within a bundle that are oxidized to the same degree.     

An Improved Method for Solution of the Linear Diffusion Equation

Abstract

The diffusion equation is often solved numerically, using finite-difference techniques. The linear one-dimensional diffusion equation may also be solved by the method of variation of parameters, which yields a solution in the form of an infinite series of products of eigenfunctions and time-varying coefficients. The series form of solution has advantages over finite-difference techniques, but the rate of convergence is often not sufficient to yield useful results. This paper presents an alternative form of the series solution for one-dimensional, Cartesian, homogeneous, and temperature-independent property conditions. The methodology, which exhibits excellent convergence, is applicable to several related problems.     

Thermodynamic Measurements in a Strongly Interacting Fermi Gas

Strongly interacting Fermi gases provide a clean and controllable laboratory system for modeling strong interparticle interactions between fermions in nature, from high temperature superconductors to neutron matter and quark-gluon plasmas. Model-independent thermodynamic measurements, which do not require theoretical models for calibrations, are very important for exploring this important system experimentally, as they enable direct tests of predictions based on the best current non-perturbative many-body theories. At Duke University, we use all-optical methods to produce a strongly interacting Fermi gas of spin-1/2-up and spin-1/2-down ⁶Li atoms that is magnetically tuned near a collisional (Feshbach) resonance. We conduct a series of measurements on the thermodynamic properties of this unique quantum gas, including the energy E, entropy S, and sound velocity  c. Our model-independent measurements of E and S enable a precision study of the finite temperature thermodynamics. The E(S) data are directly compared to several recent predictions. The temperature in both the superfluid and normal fluid regime is obtained from the fundamental thermodynamic relation T=? E/? S by parameterizing the E(S) data using two different power laws that are joined with continuous E and T at a certain entropy S _c, where the fit is optimized. We observe a significant change in the scaling of E with S above and below S _c. Taking the fitted value of S _c as an estimate of the critical entropy for a superfluid-normal fluid phase transition in the strongly interacting Fermi gas, we estimate the critical parameters. Our E(S) data are also used to experimentally calibrate the endpoint temperatures obtained for adiabatic sweeps of the magnetic field between the ideal and strongly interacting regimes. This enables the first experimental calibration of the temperature scale used in experiments on fermionic pair condensation, where the ideal Fermi gas temperature is measured before sweeping the magnetic field to the strongly interacting regime. Our calibration shows that the ideal gas temperature measured for the onset of pair condensation corresponds closely to the critical temperature T _c estimated in the strongly interacting regime from the fits to our E(S) data. We also calibrate the empirical temperature employed in studies of the heat capacity and obtain nearly the same T _c. We determine the ground state energy by three different methods, using sound velocity measurements, by extrapolating E(S) to S=0 and by measuring the ratio of the cloud sizes in the strongly and weakly interacting regimes. The results are in very good agreement with recent predictions. Finally, using universal thermodynamic relations, we estimate the chemical potential and heat capacity of the trapped gas from the E(S) data.