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101.
1. n-Butyrate, a short chain fatty acid produced by colonic fermentation, induces differentiation in human neoplastic cell lines, and reduces expression in vitro of a sialyltransferase that glycosylates N-linked glycoproteins in hepatoblastoma cells. Gangliosides are amphipathic, sialylated glycosphingolipids that undergo profound changes in many transformed cells and may protect neoplastic cells from host immune surveillance. Colonic mucosal cells are exposed to luminal short-chain fatty acid concentrations of up to 80 mmol/l, and there is some evidence that short-chain fatty acids may alter ganglioside expression in colon cancer cells. 2. Because of the importance of gangliosides in cancer pathogenesis, we investigated the effects of n-butyrate on ganglioside expression of colonic (human and murine) and non-colonic cancer cells. 3. Three separate colon cancer cell lines (LS174T, T84 and MCA-38), when butyrate treated, demonstrated striking amplification of specific individual gangliosides. However, the total lipid-bound sialic acid content of gangliosides of butyrate-treated LS174T cells diminished. In contrast to earlier reports, n-butyrate did not mediate expression of all gangliosides and specifically did not mediate expression of GM3. This effect persisted even after removal of butyrate. 4. In contrast, exposure of extracolonic cells to butyrate, including cervical cancer (HeLa) and laryngeal cancer (HEp-2) cell lines in this study and hepatoblastoma cells (Hep G2) in our previous work, caused no detectable changes in ganglioside expression. 5. In conclusion, our results indicate a relative tissue specificity of butyrate-mediated alterations in ganglioside expression that is not universal but is limited to specific gangliosides. 相似文献
102.
J. D. Warthen C.-J. Lee E. B. Jang D. R. Lance D. O. McInnis 《Journal of chemical ecology》1997,23(7):1891-1900
Twenty-eight volatile compounds from freshly crushed, ripe, dark red coffee fruit, Coffea arabica, were identified by dynamic headspace analysis techniques. Identifications were made on the basis of a comparison of Kovats indices and GC-MS spectra for unknowns and authentic samples. Of the compounds identified, 10 were alcohols, nine were aldehydes, five were ketones, and four were monoterpenes. The five most abundant volatiles in decreasing order were hexanal (21%), 2-(E)-hexenal (11%), 3-methyl-1-butanol (9.0%), 3-methyl-1-butanal (8.5%), and 1-hexanol (8.4%). The five least abundant volatiles of the 28 identified, in increasing order, were decanal (0.19%), methyl hexanoate (0.33%), pulegone (0.44%), α-isomenthone (0.45%), and 2-nonanone (0.55%). In preliminary tests, many of the identified volatiles attracted more female Mediterranean fruit flies than the control. 相似文献
103.
Thomas P. Sullivan Lance O. Nordstrom Druscilla S. Sullivan 《Journal of chemical ecology》1985,11(7):903-919
The effectiveness of predator odors (fecal, urine, and anal scent gland) in suppressing feeding damage by snowshoe hares was investigated in pen bioassays at the University of British Columbia Research Forest, Maple Ridge, British Columbia, Canada. A total of 28 bioassay trials tested the effects of these odors on hare consumption of willow browse and coniferous seedlings. Lynx and bobcat feces, weasel anal gland secretion, and lynx, bobcat, wolf, coyote, fox, and wolverine urines resulted in the most effective suppression of hare feeding damage. Novel odors of domestic dog urine and 2-methylbutyric acid did not reduce feeding. A field bioassay with lodgepole pine seedlings and weasel scent provided significant results comparable to the pen bioassays. The short-term (up to seven days) effectiveness of these treatments was more likely due to evaporative loss of the active repellent components of a given odor than habituation of hares to the stimulus. Predator odors as repellents have a biological basis compared with the anthropomorphic origins of commercial repellents. When encapsulated in weather-proof controlled-release devices, these odors could provide long-term protection for forestry plantations and agricultural crops which experience hare/rabbit feeding damage. 相似文献
104.
Ulrike G. Lehmann Lance E. De Long Hau H. Wang K. Douglas Carlson Jack M. Williams 《Journal of Superconductivity and Novel Magnetism》1994,7(5):793-797
Vibrating reed measurements were performed on single-crystal samples ofκ-(ET)2- Cu[N(CN)2]Br in an applied magnetic fieldH oriented either parallel (“longitudinal”) or perpendicular (“transverse”) to the highly conducting ac-plane. Field-cooling data taken for the longitudinal orientation andH<0.7 T revealed a peak with a low-temperature shoulder in the reed dissipation 1/Q located at temperatureT Q below the superconducting transition temperatureT c (≈11.6 forH=0). The shoulder disappeared nearH≈0.7 T, accompanied by an abrupt change in the slope ofT Q (H), corresponding to a similar change in the slope of the upper critical magnetic fieldH c2 measured by Kwoket al. The existence of the shoulder in the dissipation peak bears on a number of current explanations for the exotic superconducting properties ofκ-(ET)2Cu[N(CN)2]Br.T Q data taken for the transverse orientation fell far below estimates ofH c2., indicating the existence of a substantial region of flux line (FL) mobility belowH c2(T). The location of a peak in NMR relaxation observed by De Sotoet al. lies close toT Q for the transverse orientation. 相似文献
105.
We offer cooperative learning workshops to all students enrolled in our largest lower division math and science courses where failure rates are high. Participation in the workshops is related to retention and higher academic achievement among engineering students of various abilities. In the tradition of workshops developed for disadvantaged students in mathematics, science, and engineering, our students meet outside of class in structured group meetings. Our contributions to this tradition include application to a broader range of courses, the inclusion of all types of students, and enhanced training of workshop facilitators in small group communication methods and modern leadership techniques. Our approach is readily adaptable to most institutions. 相似文献
106.
D. R. Lance 《Journal of chemical ecology》1992,18(7):1227-1237
FemaleDiabrotica virgifera virgifera LeConte were allowed to choose between oviposition substrates that were and those that were not associated with potential sources of semiochemicals. Females deposited over five times more eggs on moist towelettes that were treated with homogenates of female abdomens than on towelettes treated with distilled water. Similar results were obtained when screening separated the homogenates from the towelettes, indicating that odors alone could elicit the response. In contrast, females did not choose towelettes that had previously been used for oviposition or towelettes containing eggs over unused towelettes. Further tests with homogenates of abdomens and a bacteriostatic agent (sorbate) indicated that the females were probably responding to bacterial odors rather than an oviposition-enhancing pheromone. Four strains of bacteria were isolated from a homogenate of female abdomens; females deposited 4 to 16 times more eggs on substrates with odors of the bacteria than on substrates with odors of uninoculated nutrient agar. In no-choice tests, bacterial odors did not increase the number of eggs deposited per female beetle; however, in choice tests with dishes that tended to retain any beetles that entered, there were more eggs per female (but not more beetles) after 24 hr in dishes with bacterial odors than in those without the odors. Females also chose dishes with odors of excised maize (Zea mays L.) roots or elevated levels of carbon dioxide over control dishes.Mention of a commercial or proprietary product does not constitute an endorsement for its use by USDA. 相似文献
107.
The stresses and deformations in a thin ring-disk shrink fit assembly have been analyzed. The interface pressure for the presumed plane stress state has been found as a function of the interference of the fit. Interferences large enough to induce plastic deformations in the ring are accounted for. The ring material was assumed to be a linear work-hardening material that obeys Tresca's yield condition. For design purposes a graph of interface pressure and hence the hydrostatic stress state in the disk, as a function of interference, is provided. 相似文献
108.
Barreiras Nuno Correia Francisco Nunes Matos Rafaela Saldanha 《Water Resources Management》2019,33(14):4939-4953
Water Resources Management - Portugal recently faced a severe financial crisis that led to the suspension of funding for water monitoring programmes of the Portuguese National Water Resources... 相似文献
109.
Ryuichiro Shinohara Lance Ouellette Peter Nowell Chris T. Parsons Shin-ichiro S. Matsuzaki R. Paul Voroney 《Journal of Great Lakes research》2018,44(3):527-534
We examined the fractions of particulate phosphorus (PP) in the lower reaches of the Grand River, Canada, to test the hypothesis that the river is a source of both particulate-bound orthophosphate and labile species of PP. At the mouth of the Grand River, the proportion of particulate organic P (POP) was, on average, 57.7% of total PP, which was significantly higher than the proportion of particulate inorganic P (PIP) in PP. Analysis with 31P nuclear magnetic resonance (NMR) spectroscopy showed that the proportion of P species other than orthophosphate in the NaOH- EDTA extract was 1.75 times greater than that of orthophosphate. Labile P species (e.g. nucleotides and pyrophosphate) were present in the NMR spectrum; whereas, refractory organic P (myo-inositol hexakisphosphate) was absent. These results suggest that during winter and spring, the Grand River supplies primarily bioavailable phosphorus species in organic forms to Lake Erie, rather than inorganic orthophosphate. These results suggest that labile organic P is contained in PP rather than alkaline extractable inorganic P. Future studies should examine POP species in other rivers of the Lake Erie watershed. 相似文献
110.
Ricardo?J.?M.?de?Carvalho Maria?C.?W.?Saldanha Mario?C.?R.?Vidal Paulo?V.?R.?CarvalhoEmail author 《Cognition, Technology & Work》2016,18(2):403-422
This paper describes an exploratory situated approach for the design, development, standardization, and implementation of line-oriented flight training (LOFT) in a major airline. LOFT was conceived in aviation industry to be a practical application of crew resource management (CRM) concepts. The situated approach combines a set of methods and techniques from ergonomics and human factors disciplines. These methods were supported by social construction enabling the involvement and participation of different actors of the operational, tactical, and strategic level of the company. Under this framework, situated design is progressively established by socially constructed patterns up to a situated design comprising the construction of scenarios, training tools, procedures, structures, organization, flight documents, operations, and further training management contents. Our findings indicate that LOFT situated design, framed by social construction, can be applied to any aviation system with a specific culture and organization, which may be different from the ones that were implicitly or explicitly taken into account during the development of general LOFT guidelines. 相似文献