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101.
Sourabh Ghosh Sara T. Parker Xianyan Wang David L. Kaplan Jennifer A. Lewis 《Advanced functional materials》2008,18(13):1883-1889
Three–dimensional, microperiodic scaffolds of regenerated silk fibroin have been fabricated for tissue engineering by direct ink writing. The ink, which consisted of silk fibroin solution from the Bombyx mori silkworm, was deposited in a layer‐by‐layer fashion through a fine nozzle to produce a 3D array of silk fibers of diameter 5 µm. The extruded fibers crystallized when deposited into a methanol‐rich reservoir, retaining a pore structure necessary for media transport. The rheological properties of the silk fibroin solutions were investigated and the crystallized silk fibers were characterized for structure and mechanical properties by infrared spectroscopy and nanoindentation, respectively. The scaffolds supported human bone marrow‐derived mesenchymal stem cell (hMSC) adhesion, and growth. Cells cultured under chondrogenic conditions on these scaffolds supported enhanced chondrogenic differentiation based on increased glucosaminoglycan production compared to standard pellet culture. Our results suggest that 3D silk fibroin scaffolds may find potential application as tissue engineering constructs due to the precise control of their scaffold architecture and their biocompatibility. 相似文献
102.
F. Infante P. Perdu H.B. Kor C.L. Gan D. Lewis 《Microelectronics Reliability》2011,51(9-11):1684-1688
Magnetic microscopy has proven its usefulness throughout the years. It allows current localization with a certain degree of precision by using an inversion algorithm to invert the Biot–Savart law. The goal is to obtain the current distribution once the magnetic field is given. However, in order to obtain a stable solution, the magnetic data is severely low-pass filtered in the spatial Fourier domain, and some important information is lost. In this paper, the contribution given by the different spatial frequencies was studied: it was demonstrated how this information can be used to obtain additional information regarding the position of the currents. A comparative study between the theoretical approach and the application to the measurements is also shown. 相似文献
103.
Alex Chortos Jie Mao Jochen Mueller Ehsan Hajiesmaili Jennifer A. Lewis David R. Clarke 《Advanced functional materials》2021,31(22):2010643
Active soft materials that change shape on demand are of interest for a myriad of applications, including soft robotics, biomedical devices, and adaptive systems. Despite recent advances, the ability to rapidly design and fabricate active matter in complex, reconfigurable layouts remains challenging. Here, the 3D printing of core-sheath-shell dielectric elastomer fibers (DEF) and fiber bundles with programmable actuation is reported. Complex shape morphing responses are achieved by printing individually addressable fibers within 3D architectures, including vertical coils and fiber bundles. These DEF devices exhibit resonance frequencies up to 700 Hz and lifetimes exceeding 2.6 million cycles. The multimaterial, multicore-shell 3D printing method opens new avenues for creating active soft matter with fast programable actuation. 相似文献
104.
A parameter tolerance, signal to noise ratio comparison is made between the MASH third order sigma-delta modulator structure and a new fourth order cascaded structure. The fourth order structure meets the required performance specification with wider parameter tolerances, allowing easier integration using hybrid CMOS technology.<> 相似文献
105.
A low-abundance form of water, H(2)(17)O, was enriched from 0.04% to ~90% by slow evaporation and fractional distillation of tap water. The density and refractive index for H(2)(17)O are reported. Gas chromatography-mass spectrometry (GC-MS) of (16)O- and (17)O-1-hexanols and their trimethyl silyl ethers and of (16)O- and (17)O-hexamethyl disiloxanes was used to determine the percentage of (17)O enrichment in the H(2)(17)O. Furthermore, the chemical shifts of labeled and nonlabeled water dissolved in CDCl(3) differed sufficiently that we could verify the enrichment of H(2)(17)O. (17)O hexanol was synthesized by the reaction of iodohexane with Na(17)OH. (17)O-Labeled trimethylsilanol and (17)O-labeled hexamethyldisiloxane were prepared by the reaction of H(2)(17)O with bis(trimethylsilyl)trifluoroacetamide (BSTFA). To generate standards for (17)O NMR, H(2)(17)O(2), and (17)O camphor were prepared. H(2)(17)O was electrolyzed to form (17)O-labeled hydrogen peroxide which was quantified using two colorimetric assays. (17)O-Labeled camphor was prepared by exchanging the ketone oxygen of camphor using H(2)(17)O. The (17)O-labeled compounds were characterized using (17)O, (1)H, and (13)C NMR and GC-MS. While we were characterizing the labeled camphor, we also detected an unexpected oxygen exchange reaction of primary alcohols, catalyzed by electrophilic ketones such as camphor. The reaction is a displacement of the alcohol OH group by water. This is an example of the usefulness of (17)O NMR in the study of a reaction mechanism that has not been noticed previously. 相似文献
106.
V. Quintard B. Parmentier T. Phan D. Lewis S. Dilhaire W. Claeys 《Quality and Reliability Engineering International》1996,12(6):447-452
We present the results of a non-destructive measuring method allowing us to characterize the evolution of solder joints during thermal cycling ageing tests. The method uses a high resolution optical probe to detect selectively pure Joule and Peltier thermal responses of the solder joint subject to a given current pulse. The results show the Peltier and Joule responses to be good indicators for the evaluation of the age and the degradation of solder joints. 相似文献
107.
Frank Greenway Brian Loveridge Richard M. Grimes Tori R. Tucker Michael Alexander Scott A. Hepford Justin Fontenot Candi Nobles-James Carol Wilson Adam M. Starr Mohammed Abdelsaid Stanley T. Lewis Jonathan R. T. Lakey 《International journal of molecular sciences》2022,23(3)
Prevalence of type 2 diabetes increased from 2.5% of the US population in 1990 to 10.5% in 2018. This creates a major public health problem, due to increases in long-term complications of diabetes, including neuropathy, retinopathy, nephropathy, skin ulcers, amputations, and atherosclerotic cardiovascular disease. In this review, we evaluated the scientific basis that supports the use of physiologic insulin resensitization. Insulin resistance is the primary cause of type 2 diabetes. Insulin resistance leads to increasing insulin secretion, leading to beta-cell exhaustion or burnout. This triggers a cascade leading to islet cell destruction and the long-term complications of type 2 diabetes. Concurrent with insulin resistance, the regular bursts of insulin from the pancreas become irregular. This has been treated by the precise administration of insulin more physiologically. There is consistent evidence that this treatment modality can reverse the diabetes-associated complications of neuropathy, diabetic ulcers, nephropathy, and retinopathy, and that it lowers HbA1c. In conclusion, physiologic insulin resensitization has a persuasive scientific basis, significant treatment potential, and likely cost benefits. 相似文献
108.
Numerical models of various donor/attenuator systems utilized in gap tests were evaluated with the DYNA2D hydrocode. Three donor (pressed pentolite)/attenuator (PMMA) configurations were tested. Case I and Case 2 had a pentolite donor which was 2 cm × 2 cm right cylinder; the cell size was smaller in Case 1. Case 3 utilized a composite pentolite donor 2.07 cm long but having stepped diameters of one and two cm; the cell size was quite similar to that of Case 2. Peak (axial) pressure versus distance results from Case 1 were scaled and Compared to experimental data for both the NOL large scale gap test (LSGT) and the NSWC expanded large scale gap test (ELSGT). The comparison was found to be very good for the LSGT but less satisfactory for the ELSGT. Peak (axial) pressure versus distance results from Case 1 were the also compared to Case 2, a coarser zoned model similar to Case 1, and Case 3. This comparison showed that the composite donor produced peak (axial) pressures similar to those from the LSGT model. Normalized peak pressure versus radial distance and shock profiles were compared for the three cases and found to be quite similar. Additional normalized peak pressure versus radial distance and normalized pressure versus time information from Case 1 is plotted to show how the pentolite/PMMA rarefaction affects both the axial and radial pressure-time histories. 相似文献
109.
Alexandre Joushomme Andr Garenne Mlody Dufosse Rmy Renom Hermanus Johannes Ruigrok Yann Loick Chappe Anne Canovi Lorenza Patrignoni Annabelle Hurtier Florence Poulletier de Gannes Isabelle Lagroye Philippe Lvêque Noëlle Lewis Muriel Priault Delia Arnaud-Cormos Yann Percherancier 《International journal of molecular sciences》2022,23(2)
It remains controversial whether exposure to environmental radiofrequency signals (RF) impacts cell status or response to cellular stress such as apoptosis or autophagy. We used two label-free techniques, cellular impedancemetry and Digital Holographic Microscopy (DHM), to assess the overall cellular response during RF exposure alone, or during co-exposure to RF and chemical treatments known to induce either apoptosis or autophagy. Two human cell lines (SH-SY5Y and HCT116) and two cultures of primary rat cortex cells (astrocytes and co-culture of neurons and glial cells) were exposed to RF using an 1800 MHz carrier wave modulated with various environmental signals (GSM: Global System for Mobile Communications, 2G signal), UMTS (Universal Mobile Telecommunications System, 3G signal), LTE (Long-Term Evolution, 4G signal, and Wi-Fi) or unmodulated RF (continuous wave, CW). The specific absorption rates (S.A.R.) used were 1.5 and 6 W/kg during DHM experiments and ranged from 5 to 24 W/kg during the recording of cellular impedance. Cells were continuously exposed for three to five consecutive days while the temporal phenotypic signature of cells behavior was recorded at constant temperature. Statistical analysis of the results does not indicate that RF-EMF exposure impacted the global behavior of healthy, apoptotic, or autophagic cells, even at S.A.R. levels higher than the guidelines, provided that the temperature was kept constant. 相似文献
110.